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Borrelia Lusitaniae
''Borrelia lusitaniae'' is a bacterium of the spirochete class of the genus'' Borrelia'', which has a diderm (double-membrane) envelope. It is a part of the ''Borrelia burgdorferi'' ''sensu lato'' genospecies and is a Gram-negative bacterium. ''B. lusitaniae'' is tick-borne; he type strain is PotiB2. It can be pathogenic, being involved in cases of Lyme borreliosis. A species of tick, ''Ixodes ricinus'', is the host of ''B. lusitaniae''. It is thought to have originated from Portugal and has since spread to parts of Europe and North Africa. Lizards of the family Lacertidae are now believed to be important reservoir hosts of this bacterium. Origin and distribution While ''B. lusitaniae'' is distributed throughout countries in Europe and North Africa, it is the sole species of the Lyme borreliosis group in southern Portugal. Lizards of the family Lacertidae are believed to be important reservoir hosts of ''B. lusitaniae''. They were first isolated in Portugal in 1993. These lizar ...
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Spirochete
A spirochaete () or spirochete is a member of the phylum Spirochaetota (also called Spirochaetes ), which contains distinctive diderm (double-membrane) Gram-negative bacteria, most of which have long, helically coiled (corkscrew-shaped or spiraled, hence the name) cells. Spirochaetes are chemoheterotrophic in nature, with lengths between 3 and 500 μm and diameters around 0.09 to at least 3 μm. Spirochaetes are distinguished from other bacterial phyla by the location of their flagella, called endoflagella, or periplasmic flagella, which are sometimes called ''axial filaments''. Endoflagella are anchored at each end (pole) of the bacterium within the periplasmic space (between the inner and outer membranes) where they project backwards to extend the length of the cell. These cause a twisting motion which allows the spirochaete to move. When reproducing, a spirochaete will undergo asexual transverse binary fission. Most spirochaetes are free-living and anaerob ...
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Microbiology And Immunology
Microbiology () is the scientific study of microorganisms, those being of unicellular (single-celled), multicellular (consisting of complex cells), or acellular (lacking cells). Microbiology encompasses numerous sub-disciplines including virology, bacteriology, protistology, mycology, immunology, and parasitology. The organisms that constitute the microbial world are characterized as either prokaryotes or eukaryotes; eukaryote, Eukaryotic microorganisms possess membrane-bound organelles and include fungi and protists, whereas prokaryote, prokaryotic organisms are conventionally classified as lacking membrane-bound organelles and include Bacteria and Archaea. Microbiologists traditionally relied on culture, staining, and microscopy for the isolation and identification of microorganisms. However, less than 1% of the microorganisms present in common environments can be cultured in isolation using current means. With the emergence of biotechnology, Microbiologists currently rely on ...
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Plasmid
A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria and archaea; however plasmids are sometimes present in and eukaryotic organisms as well. Plasmids often carry useful genes, such as those involved in antibiotic resistance, virulence, secondary metabolism and bioremediation. While chromosomes are large and contain all the essential genetic information for living under normal conditions, plasmids are usually very small and contain additional genes for special circumstances. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms. In the laboratory, plasmids may be introduced into a cell via transformation. Synthetic plasmids are available for procurement over the internet by various vendors ...
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Serological
Serology is the scientific study of serum and other body fluids. In practice, the term usually refers to the diagnostic identification of antibodies in the serum. Such antibodies are typically formed in response to an infection (against a given microorganism), against other foreign proteins (in response, for example, to a mismatched blood transfusion), or to one's own proteins (in instances of autoimmune disease). In either case, the procedure is simple. Serological tests Serological tests are diagnostic methods that are used to identify antibodies and antigens in a patient's sample. Serological tests may be performed to diagnose infections and autoimmune illnesses, to check if a person has immunity to certain diseases, and in many other situations, such as determining an individual's blood type. Serological tests may also be used in forensic serology to investigate crime scene evidence. Several methods can be used to detect antibodies and antigens, including ELISA, agglutinatio ...
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Lisbon
Lisbon ( ; ) is the capital and largest city of Portugal, with an estimated population of 567,131, as of 2023, within its administrative limits and 3,028,000 within the Lisbon Metropolitan Area, metropolis, as of 2025. Lisbon is mainland Europe's westernmost capital city (second overall after Reykjavík, Reykjavik), and the only one along the Atlantic coast, the others (Reykjavik and Dublin) being on islands. The city lies in the western portion of the Iberian Peninsula, on the northern shore of the River Tagus. The western portion of its metro area, the Portuguese Riviera, hosts the westernmost point of Continental Europe, culminating at Cabo da Roca. Lisbon is one of the List of oldest continuously inhabited cities, oldest cities in the world and the second-oldest European capital city (after Athens), predating other modern European capitals by centuries. Settled by pre-Celtic tribes and later founded and civilized by the Phoenicians, Julius Caesar made it a municipium ...
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Mafra, Portugal
Mafra () is a city and a municipality in the Lisbon District, district of Lisbon, on the west coast of Portugal, and part of the urban agglomeration of the Grande Lisboa, Greater Lisbon subregion. The population in 2011 was 76,685, in an area of 291.66 km2. It is mostly known for the sumptuous Mafra National Palace inscribed as a UNESCO World Heritage Site. Built in the baroque style, the Mafra National Palace also inspired Portuguese Nobel Prize laureate José Saramago to write his novel ''Baltasar and Blimunda'' (Memorial do Convento). Other points of interest around the municipality include the Tapada Nacional de Mafra (also part of the UNESCO site), an enclosed wildlife and game reserve, and Ericeira's World Surf Reserve, the second in the world. History The earliest archaeological remnants discovered in Mafra date to an early settlement of this region in the Neolithic period. In Seixosa, civil parish of Encarnação (Mafra), Encarnação, in an area that was once a beach ...
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Belarus
Belarus, officially the Republic of Belarus, is a landlocked country in Eastern Europe. It is bordered by Russia to the east and northeast, Ukraine to the south, Poland to the west, and Lithuania and Latvia to the northwest. Belarus spans an area of with a population of . The country has a hemiboreal climate and is administratively divided into Regions of Belarus, six regions. Minsk is the capital and List of cities and largest towns in Belarus, largest city; it is administered separately as a city with special status. For most of the medieval period, the lands of modern-day Belarus was ruled by independent city-states such as the Principality of Polotsk. Around 1300 these lands came fully under the Grand Duchy of Lithuania and subsequently by the Polish–Lithuanian Commonwealth; this period lasted for 500 years until the Partitions of Poland, 1792-1795 partitions of Poland-Lithuania placed Belarus within the Belarusian history in the Russian Empire, Russian Empire for the fi ...
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Ukraine
Ukraine is a country in Eastern Europe. It is the List of European countries by area, second-largest country in Europe after Russia, which Russia–Ukraine border, borders it to the east and northeast. Ukraine also borders Belarus to the north; Poland and Slovakia to the west; Hungary, Romania and Moldova to the southwest; and the Black Sea and the Sea of Azov to the south and southeast. Kyiv is the nation's capital and List of cities in Ukraine, largest city, followed by Kharkiv, Odesa, and Dnipro. Ukraine's official language is Ukrainian language, Ukrainian. Humans have inhabited Ukraine since 32,000 BC. During the Middle Ages, it was the site of early Slavs, early Slavic expansion and later became a key centre of East Slavs, East Slavic culture under the state of Kievan Rus', which emerged in the 9th century. Kievan Rus' became the largest and most powerful realm in Europe in the 10th and 11th centuries, but gradually disintegrated into rival regional powers before being d ...
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Moldavia
Moldavia (, or ; in Romanian Cyrillic alphabet, Romanian Cyrillic: or ) is a historical region and former principality in Eastern Europe, corresponding to the territory between the Eastern Carpathians and the Dniester River. An initially independent and later autonomous state, it existed from the 14th century to 1859, when it united with Wallachia () as the basis of the modern Romanian state; at various times, Moldavia included the regions of Bessarabia (with the Budjak), all of Bukovina and Hertsa region , Hertsa. The region of Pokuttya was also part of it for a period of time. The Moldavia (region of Romania) , western half of Moldavia is now part of Romania, the eastern side belongs to the Moldova , Republic of Moldova, and the Chernivtsi Oblast , northern and Budjak , southeastern parts are territories of Ukraine. Name and etymology The original and short-lived reference to the region was ''Bogdania'', after Bogdan I, the founding figure of the principality. The name ...
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Czech Republic
The Czech Republic, also known as Czechia, and historically known as Bohemia, is a landlocked country in Central Europe. The country is bordered by Austria to the south, Germany to the west, Poland to the northeast, and Slovakia to the southeast. The Czech Republic has a hilly landscape that covers an area of with a mostly temperate Humid continental climate, continental and oceanic climate. The capital and largest city is Prague; other major cities and urban areas include Brno, Ostrava, Plzeň and Liberec. The Duchy of Bohemia was founded in the late 9th century under Great Moravia. It was formally recognized as an Imperial Estate of the Holy Roman Empire in 1002 and became Kingdom of Bohemia, a kingdom in 1198. Following the Battle of Mohács in 1526, all of the Lands of the Bohemian Crown were gradually integrated into the Habsburg monarchy. Nearly a hundred years later, the Protestantism, Protestant Bohemian Revolt led to the Thirty Years' War. After the Battle of White ...
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Housekeeping Genes
In molecular biology, housekeeping genes are typically constitutive genes that are required for the maintenance of basic cellular function, and are gene expression, expressed in all cells of an organism under normal and patho-physiological conditions. Although some housekeeping genes are expressed at relatively constant rates in most non-pathological situations, the expression of other housekeeping genes may vary depending on experimental conditions. The origin of the term "housekeeping gene" remains obscure. Literature from 1976 used the term to describe specifically tRNA and rRNA. For experimental purposes, the expression of one or multiple housekeeping genes is used as a reference point for the analysis of expression levels of other genes. The key criterion for the use of a housekeeping gene in this manner is that the chosen housekeeping gene is uniformly expressed with low variance under both control and experimental conditions. Validation of housekeeping genes should be per ...
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