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Australian Bat Lyssavirus
Australian bat lyssavirus (ABLV) is a enzootic virus closely related to the rabies virus. It was first identified in a 5-month-old juvenile black flying fox (''Pteropus alecto'') collected near Ballina, New South Wales, Ballina in northern New South Wales, Australia, in January 1995 during a national surveillance program for the recently identified Hendra virus. ABLV is the seventh member of the genus ''Lyssavirus'' (which includes Rabies virus) and the only ''Lyssavirus'' member present in Australia. ABLV has been categorized to the Phylogroup I of the Lyssaviruses. Virology Molecular structure The Australian bat lyssavirus (ABLV) shares many structural characteristics with the other Lyssaviruses, despite being genetically and serologically distinct from the others. Visually, ABLV is a bullet shaped virus. Molecularly, ABLV is an enveloped, negative-sense, single-stranded RNA virus. The (-)ssRNA genome is relatively small, containing 12kilobases of genetic material and encod ...
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Transmission Electron Micrograph
Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The image is then magnified and Focus (optics), focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a Detectors for transmission electron microscopy, detector such as a scintillator attached to a charge-coupled device or a direct electron detector. Transmission electron microscopes are capable of imaging at a significantly higher Optical resolution, resolution than Optical microscope, light microscopes, owing to the smaller de Broglie wavelength of electrons. This enables the instrument to capture fine detail—even as small as a single column of atoms, which is thousands o ...
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Pteropus
''Pteropus'' (suborder Yinpterochiroptera) is a genus of megabats which are among the largest bats in the world. They are commonly known as fruit bats or flying foxes, among other colloquial names. They live in South Asia, Southeast Asia, Australia, East Africa, and some oceanic islands in the Indian and Pacific Oceans. There are at least 60 extant species in the genus. Flying foxes eat fruit and other plant matter, and occasionally consume insects as well. They locate resources with their keen sense of smell. Most, but not all, are nocturnal. They navigate with keen eyesight, as they cannot echolocate. They have long life spans and low reproductive outputs, with females of most species producing only one offspring per year. Their slow life history makes their populations vulnerable to threats such as overhunting, culling, and natural disasters. Six flying fox species have been made extinct in modern times by overhunting. Flying foxes are often persecuted for their real or ...
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Immunohistochemistry
Immunohistochemistry is a form of immunostaining. It involves the process of selectively identifying antigens in cells and tissue, by exploiting the principle of Antibody, antibodies binding specifically to antigens in biological tissues. Albert Coons, Albert Hewett Coons, Ernst Berliner, Ernest Berliner, Norman Jones and Hugh J Creech was the first to develop immunofluorescence in 1941. This led to the later development of immunohistochemistry. Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. In some cancer cells certain tumor antigens are expressed which make it possible to detect. Immunohistochemistry is also widely used in basic research, to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. Sample preparation Immunohistochemistry can be performed on tissue that has been fixed and embedded in Paraffin wax, paraffin, ...
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Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation. Mullis and biochemist Michael Smith (chemist), Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of Ancient DNA, ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications including biomedical research and forensic ...
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Electroencephalography
Electroencephalography (EEG) is a method to record an electrogram of the spontaneous electrical activity of the brain. The biosignal, bio signals detected by EEG have been shown to represent the postsynaptic potentials of pyramidal neurons in the neocortex and allocortex. It is typically non-invasive, with the EEG electrodes placed along the scalp (commonly called "scalp EEG") using the 10–20 system (EEG), International 10–20 system, or variations of it. Electrocorticography, involving surgical placement of electrodes, is sometimes called Electrocorticography, "intracranial EEG". Clinical interpretation of EEG recordings is most often performed by visual inspection of the tracing or quantitative EEG, quantitative EEG analysis. Voltage fluctuations measured by the EEG bioamplifier, bio amplifier and electrodes allow the evaluation of normal Brain activity and meditation, brain activity. As the electrical activity monitored by EEG originates in neurons in the underlying Huma ...
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Dysphagia
Dysphagia is difficulty in swallowing. Although classified under " symptoms and signs" in ICD-10, in some contexts it is classified as a condition in its own right. It may be a sensation that suggests difficulty in the passage of solids or liquids from the mouth to the stomach, a lack of pharyngeal sensation or various other inadequacies of the swallowing mechanism. Dysphagia is distinguished from other symptoms including odynophagia, which is defined as painful swallowing, and globus, which is the sensation of a lump in the throat. A person can have dysphagia without odynophagia (dysfunction without pain), odynophagia without dysphagia (pain without dysfunction) or both together. A psychogenic dysphagia is known as phagophobia. Classification Dysphagia is classified into the following major types: # Oropharyngeal dysphagia # Esophageal and obstructive dysphagia # Neuromuscular symptom complexes # Functional dysphagia is defined in some patients as having no organic c ...
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Diplopia
Diplopia is the simultaneous perception of two images of a single object that may be displaced in relation to each other. Also called double vision, it is a loss of visual focus under regular conditions, and is often voluntary. However, when occurring involuntarily, it results from impaired function of the extraocular muscles, where both eyes are still functional, but they cannot turn to target the desired object. Problems with these muscles may be due to mechanical problems, disorders of the neuromuscular junction, disorders of the cranial nerves ( III, IV, and VI) that innervate the muscles, and occasionally disorders involving the supranuclear oculomotor pathways or ingestion of toxins. Diplopia can be one of the first signs of a systemic disease, particularly to a muscular or neurological process, and it may disrupt a person's balance, movement, or reading abilities. Causes Diplopia has a diverse range of ophthalmologic, infectious, autoimmune, neurological, and neoplasti ...
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Yellow-bellied Sheath-tailed Bat
The yellow-bellied sheath-tailed bat (''Saccolaimus flaviventris''), also known as the yellow-bellied sheathtail or yellow-bellied pouched bat, is a microbat Microbats constitute the suborder Microchiroptera within the order Chiroptera (bats). Bats have long been differentiated into Megachiroptera (megabats) and Microchiroptera, based on their size, the use of echolocation by the Microchiroptera an ... species of the family Emballonuridae found extensively in Australia and less commonly in parts of Papua New Guinea.Flannery, T. F. 1995. ''The Mammals of New Guinea'', 2nd edition. Reed Books, Sydney, Australia.Churchill, Sue. 2008. ''Australian bats'', 2nd ed. Allen & Unwin, Crows Nest, NSW. Although found throughout most parts of Australia, very little is known about its ecology due to the small size, nocturnal activity, and general elusiveness of most microbat species, making them difficult to study.Rhodes, M. P., and Hall, L. S. 1997. Observations on Yellow-bellied sheath-t ...
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Rockhampton
Rockhampton is a city in the Rockhampton Region of Central Queensland, Australia. In the , the population of Rockhampton was 79,293. A common nickname for Rockhampton is "Rocky", and the demonym of Rockhampton is Rockhamptonite. The Scottish-Norwegians, Norwegian explorers Archer brothers, Charles and William Archer came across the Toonooba River in 1853 and named it in-honour of Sir Charles Augustus FitzRoy, Charles FitzRoy; they also named many local landmarks after figures in Norse mythology, including the Berserker Range named after the Norse warrior "Berserker, Baresark". The Archer brothers took-up a run near Gracemere, Queensland, Gracemere in 1855, and more settlers arrived soon thereafter, enticed by the fertile valleys. The town of Rockhampton was proclaimed in 1858, and surveyed to a design that closely resembled the Hoddle Grid of Melbourne, and consisted of a grid of wide boulevards and laneways, which was uncommon for Queensland cities. Within a year, gold was fou ...
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Little Red Flying Fox
The little red flying-fox (''Pteropus scapulatus'') is a megachiropteran bat native to northern and eastern Australia. The species weighs about half a kilogram, one US pound, and is the smallest species of ''Pteropus'' in mainland Australia. ''P. scapulatus'' occurs at the coast and further inland, camping and flying to the tropical to temperate regions that provide them with an annual source of nectar. They exhibit an unusual method of obtaining drinking water during dry periods, skimming a stream's surface to gather it onto their fur while they are in flight. Taxonomy The first description was published by Wilhelm Peters in 1862, as a 'new species of ''flederhund'' from New Holland'. The type specimen was collected at Cape York Peninsula. The population gives its name to the ''scapulatus'' species group', as recognised by authors in the late twentieth century. ''Pteropus scapulatus'' is well known and referred to by many names, these include the 'collared' flying-fox or fr ...
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Spectacled Flying Fox
The spectacled flying fox (''Pteropus conspicillatus''), also known as the spectacled fruit bat, is a megabat that lives in Australia's north-eastern regions of Queensland. It is also found in New Guinea and on the offshore islands including Woodlark Island, Alcester Island, Kiriwina, and Halmahera. The spectacled flying fox was listed as a threatened species under the Environment Protection and Biodiversity Conservation Act 1999. They were considered vulnerable due to a significant decline in numbers as a result of loss of their prime feeding habitat and secluded camp sites. It has also been reported that spectacled flying foxes skim over the surface of water to drink and are sometimes eaten by crocodiles. The species was classified as endangered by the IUCN in 2020. In February 2019, the Australian government upgraded the threatened status from vulnerable to endangered, after almost a third of the bat population died in a severe heatwave in Queensland in late 2018. Descripti ...
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