An experiment is a procedure carried out to support or refute a

hypothesis A hypothesis (plural hypotheses) is a proposed explanation for a phenomenon. For a hypothesis to be a scientific hypothesis, the scientific method requires that one can test it. Scientists generally base scientific hypotheses on previous obse ...

, or determine the efficacy or

likelihood The likelihood function (often simply called the likelihood) represents the probability of random variable realizations conditional on particular values of the statistical parameters. Thus, when evaluated on a given sample, the likelihood functi ...

of something previously untried. Experiments provide insight into

cause-and-effect Causality (also referred to as causation, or cause and effect) is influence by which one event, process, state, or object (''a'' ''cause'') contributes to the production of another event, process, state, or object (an ''effect'') where the ca ...

by demonstrating what outcome occurs when a particular factor is manipulated. Experiments vary greatly in goal and scale but always rely on repeatable procedure and logical analysis of the results. There also exist natural experimental studies. A child may carry out basic experiments to understand how things fall to the ground, while teams of scientists may take years of systematic investigation to advance their understanding of a phenomenon. Experiments and other types of hands-on activities are very important to student learning in the science classroom. Experiments can raise test scores and help a student become more engaged and interested in the material they are learning, especially when used over time. Experiments can vary from personal and informal natural comparisons (e.g. tasting a range of chocolates to find a favorite), to highly controlled (e.g. tests requiring complex apparatus overseen by many scientists that hope to discover information about subatomic particles). Uses of experiments vary considerably between the

natural Nature, in the broadest sense, is the physical world or universe. "Nature" can refer to the phenomena of the physical world, and also to life in general. The study of nature is a large, if not the only, part of science. Although humans are ...

and

human Humans (''Homo sapiens'') are the most abundant and widespread species of primate, characterized by bipedalism and exceptional cognitive skills due to a large and complex brain. This has enabled the development of advanced tools, cultu ...

sciences. Experiments typically include controls, which are designed to minimize the effects of variables other than the single independent variable. This increases the reliability of the results, often through a comparison between control measurements and the other measurements. Scientific controls are a part of the

scientific method The scientific method is an empirical method for acquiring knowledge that has characterized the development of science since at least the 17th century (with notable practitioners in previous centuries; see the article history of scientific ...

. Ideally, all variables in an experiment are controlled (accounted for by the control measurements) and none are uncontrolled. In such an experiment, if all controls work as expected, it is possible to conclude that the experiment works as intended, and that results are due to the effect of the tested variables.

Overview

In the

, an experiment is an empirical procedure that arbitrates competing models or hypotheses. Researchers also use experimentation to test existing

theories A theory is a rational type of abstract thinking about a phenomenon, or the results of such thinking. The process of contemplative and rational thinking is often associated with such processes as observational study or research. Theories may be ...

or new hypotheses to support or disprove them. An experiment usually tests a

, which is an expectation about how a particular process or phenomenon works. However, an experiment may also aim to answer a "what-if" question, without a specific expectation about what the experiment reveals, or to confirm prior results. If an experiment is carefully conducted, the results usually either support or disprove the hypothesis. According to some philosophies of science, an experiment can never "prove" a hypothesis, it can only add support. On the other hand, an experiment that provides a

counterexample A counterexample is any exception to a generalization. In logic a counterexample disproves the generalization, and does so rigorously in the fields of mathematics and philosophy. For example, the fact that "John Smith is not a lazy student" is a ...

can disprove a theory or hypothesis, but a theory can always be salvaged by appropriate

ad hoc Ad hoc is a Latin phrase meaning literally 'to this'. In English, it typically signifies a solution for a specific purpose, problem, or task rather than a generalized solution adaptable to collateral instances. (Compare with '' a priori''.) C ...

modifications at the expense of simplicity. An experiment must also control the possible confounding factors—any factors that would mar the accuracy or repeatability of the experiment or the ability to interpret the results. Confounding is commonly eliminated through scientific controls and/or, in

randomized experiment In science, randomized experiments are the experiments that allow the greatest reliability and validity of statistical estimates of treatment effects. Randomization-based inference is especially important in experimental design and in survey samp ...

s, through random assignment. In

engineering Engineering is the use of scientific principles to design and build machines, structures, and other items, including bridges, tunnels, roads, vehicles, and buildings. The discipline of engineering encompasses a broad range of more speciali ...

and the

physical sciences Physical science is a branch of natural science that studies non-living systems, in contrast to life science. It in turn has many branches, each referred to as a "physical science", together called the "physical sciences". Definition Phy ...

, experiments are a primary component of the scientific method. They are used to test theories and hypotheses about how physical processes work under particular conditions (e.g., whether a particular engineering process can produce a desired chemical compound). Typically, experiments in these fields focus on replication of identical procedures in hopes of producing identical results in each replication. Random assignment is uncommon. In

medicine Medicine is the science and practice of caring for a patient, managing the diagnosis, prognosis, prevention, treatment, palliation of their injury or disease, and promoting their health. Medicine encompasses a variety of health care pr ...

and the

social sciences Social science is one of the branches of science, devoted to the study of societies and the relationships among individuals within those societies. The term was formerly used to refer to the field of sociology, the original "science of so ...

, the prevalence of experimental research varies widely across disciplines. When used, however, experiments typically follow the form of the

clinical trial Clinical trials are prospective biomedical or behavioral research studies on human participants designed to answer specific questions about biomedical or behavioral interventions, including new treatments (such as novel vaccines, drugs, diet ...

, where experimental units (usually individual human beings) are randomly assigned to a treatment or control condition where one or more outcomes are assessed. In contrast to norms in the physical sciences, the focus is typically on the

average treatment effect The average treatment effect (ATE) is a measure used to compare treatments (or interventions) in randomized experiments, evaluation of policy interventions, and medical trials. The ATE measures the difference in mean (average) outcomes between units ...

(the difference in outcomes between the treatment and control groups) or another

test statistic A test statistic is a statistic (a quantity derived from the sample) used in statistical hypothesis testing.Berger, R. L.; Casella, G. (2001). ''Statistical Inference'', Duxbury Press, Second Edition (p.374) A hypothesis test is typically specifi ...

produced by the experiment. A single study typically does not involve replications of the experiment, but separate studies may be aggregated through systematic review and

meta-analysis A meta-analysis is a statistical analysis that combines the results of multiple scientific studies. Meta-analyses can be performed when there are multiple scientific studies addressing the same question, with each individual study reporting me ...

. There are various differences in experimental practice in each of the branches of science. For example, agricultural research frequently uses randomized experiments (e.g., to test the comparative effectiveness of different fertilizers), while

experimental economics Experimental economics is the application of experimental methods to study economic questions. Data collected in experiments are used to estimate effect size, test the validity of economic theories, and illuminate market mechanisms. Economic expe ...

often involves experimental tests of theorized human behaviors without relying on random assignment of individuals to treatment and control conditions.

History

One of the first methodical approaches to experiments in the modern sense is visible in the works of the Arab mathematician and scholar

Ibn al-Haytham Ḥasan Ibn al-Haytham, Latinized as Alhazen (; full name ; ), was a medieval mathematician, astronomer, and physicist of the Islamic Golden Age from present-day Iraq.For the description of his main fields, see e.g. ("He is one of the pri ...

. He conducted his experiments in the field of optics—going back to optical and mathematical problems in the works of

Ptolemy Claudius Ptolemy (; grc-gre, Πτολεμαῖος, ; la, Claudius Ptolemaeus; AD) was a mathematician, astronomer, astrologer, geographer, and music theorist, who wrote about a dozen scientific treatises, three of which were of importance ...

—by controlling his experiments due to factors such as self-criticality, reliance on visible results of the experiments as well as a criticality in terms of earlier results. He was one of the first scholars to use an inductive-experimental method for achieving results. In his '' Book of Optics'' he describes the fundamentally new approach to knowledge and research in an experimental sense: ''"We should, that is, recommence the inquiry into its principles and premisses, beginning our investigation with an inspection of the things that exist and a survey of the conditions of visible objects. We should distinguish the properties of particulars, and gather by induction what pertains to the eye when vision takes place and what is found in the manner of sensation to be uniform, unchanging, manifest and not subject to doubt. After which we should ascend in our inquiry and reasonings, gradually and orderly, criticizing premisses and exercising caution in regard to conclusions—our aim in all that we make subject to inspection and review being to employ justice, not to follow prejudice, and to take care in all that we judge and criticize that we seek the truth and not to be swayed by opinion. We may in this way eventually come to the truth that gratifies the heart and gradually and carefully reach the end at which certainty appears; while through criticism and caution we may seize the truth that dispels disagreement and resolves doubtful matters. For all that, we are not free from that human turbidity which is in the nature of man; but we must do our best with what we possess of human power. From God we derive support in all things."'' According to his explanation, a strictly controlled test execution with a sensibility for the subjectivity and susceptibility of outcomes due to the nature of man is necessary. Furthermore, a critical view on the results and outcomes of earlier scholars is necessary: Thus, a comparison of earlier results with the experimental results is necessary for an objective experiment—the visible results being more important. In the end, this may mean that an experimental researcher must find enough courage to discard traditional opinions or results, especially if these results are not experimental but results from a logical/ mental derivation. In this process of critical consideration, the man himself should not forget that he tends to subjective opinions—through "prejudices" and "leniency"—and thus has to be critical about his own way of building hypotheses.

Francis Bacon Francis Bacon, 1st Viscount St Alban (; 22 January 1561 – 9 April 1626), also known as Lord Verulam, was an English philosopher and statesman who served as Attorney General and Lord Chancellor of England. Bacon led the advancement of both ...

(1561–1626), an English philosopher and

scientist A scientist is a person who conducts scientific research to advance knowledge in an area of the natural sciences. In classical antiquity, there was no real ancient analog of a modern scientist. Instead, philosophers engaged in the philosoph ...

active in the 17th century, became an influential supporter of experimental science in the

English renaissance The English Renaissance was a cultural and artistic movement in England from the early 16th century to the early 17th century. It is associated with the pan-European Renaissance that is usually regarded as beginning in Italy in the late 14th cent ...

. He disagreed with the method of answering scientific questions by deduction—similar to

—and described it as follows: "Having first determined the question according to his will, man then resorts to experience, and bending her to conformity with his placets, leads her about like a captive in a procession." Bacon wanted a method that relied on repeatable observations, or experiments. Notably, he first ordered the scientific method as we understand it today. In the centuries that followed, people who applied the scientific method in different areas made important advances and discoveries. For example,

Galileo Galilei Galileo di Vincenzo Bonaiuti de' Galilei (15 February 1564 – 8 January 1642) was an Italian astronomer, physicist and engineer, sometimes described as a polymath. Commonly referred to as Galileo, his name was pronounced (, ). He wa ...

(1564–1642) accurately measured time and experimented to make accurate measurements and conclusions about the speed of a falling body.

Antoine Lavoisier Antoine-Laurent de Lavoisier ( , ; ; 26 August 17438 May 1794),
CNRS (

combustion Combustion, or burning, is a high-temperature exothermic redox chemical reaction between a fuel (the reductant) and an oxidant, usually atmospheric oxygen, that produces oxidized, often gaseous products, in a mixture termed as smoke. Combus ...

and

biochemistry Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and ...

and to develop the theory of

conservation of mass In physics and chemistry, the law of conservation of mass or principle of mass conservation states that for any system closed to all transfers of matter and energy, the mass of the system must remain constant over time, as the system's mass can ...

(matter). Louis Pasteur (1822–1895) used the scientific method to disprove the prevailing theory of

spontaneous generation Spontaneous generation is a superseded scientific theory that held that living creatures could arise from nonliving matter and that such processes were commonplace and regular. It was hypothesized that certain forms, such as fleas, could arise f ...

and to develop the

germ theory of disease The germ theory of disease is the currently accepted scientific theory for many diseases. It states that microorganisms known as pathogens or "germs" can lead to disease. These small organisms, too small to be seen without magnification, invade ...

. Because of the importance of controlling potentially confounding variables, the use of well-designed

laboratory A laboratory (; ; colloquially lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratory services are provided in a variety of settings: physic ...

experiments is preferred when possible. A considerable amount of progress on the design and analysis of experiments occurred in the early 20th century, with contributions from statisticians such as

Ronald Fisher Sir Ronald Aylmer Fisher (17 February 1890 – 29 July 1962) was a British polymath who was active as a mathematician, statistician, biologist, geneticist, and academic. For his work in statistics, he has been described as "a genius who ...

(1890–1962),

Jerzy Neyman Jerzy Neyman (April 16, 1894 – August 5, 1981; born Jerzy Spława-Neyman; ) was a Polish mathematician and statistician who spent the first part of his professional career at various institutions in Warsaw, Poland and then at University Colleg ...

(1894–1981),

Oscar Kempthorne Oscar Kempthorne (January 31, 1919 – November 15, 2000) was a British statistician and geneticist known for his research on randomization-analysis and the design of experiments, which had wide influence on research in agriculture, genetics, ...

(1919–2000), Gertrude Mary Cox (1900–1978), and

William Gemmell Cochran William Gemmell Cochran (15 July 1909 – 29 March 1980) was a prominent statistician. He was born in Scotland but spent most of his life in the United States. Cochran studied mathematics at the University of Glasgow and the University of Cam ...

(1909–1980), among others.

Types of experiments

Experiments might be categorized according to a number of dimensions, depending upon professional norms and standards in different fields of study. In some disciplines (e.g.,

psychology Psychology is the scientific study of mind and behavior. Psychology includes the study of conscious and unconscious phenomena, including feelings and thoughts. It is an academic discipline of immense scope, crossing the boundaries between ...

political science Political science is the scientific study of politics. It is a social science dealing with systems of governance and power, and the analysis of political activities, political thought, political behavior, and associated constitutions and la ...

), a 'true experiment' is a method of social research in which there are two kinds of variables. The independent variable is manipulated by the experimenter, and the dependent variable is measured. The signifying characteristic of a true experiment is that it randomly allocates the subjects to neutralize experimenter bias, and ensures, over a large number of iterations of the experiment, that it controls for all confounding factors. Depending on the discipline, experiments can be conducted to accomplish different but not mutually exclusive goals: test theories, search for and document phenomena, develop theories, or advise policymakers. These goals also relate differently to validity concerns.

Controlled experiments

A controlled experiment often compares the results obtained from experimental samples against ''control'' samples, which are practically identical to the experimental sample except for the one aspect whose effect is being tested (the independent variable). A good example would be a drug trial. The sample or group receiving the drug would be the experimental group (

treatment group In the design of experiments, hypotheses are applied to experimental units in a treatment group. In comparative experiments, members of a control group receive a standard treatment, a placebo, or no treatment at all. There may be more than one tr ...

); and the one receiving the

placebo A placebo ( ) is a substance or treatment which is designed to have no therapeutic value. Common placebos include inert tablets (like sugar pills), inert injections (like saline), sham surgery, and other procedures. In general, placebos can af ...

or regular treatment would be the

control Control may refer to: Basic meanings Economics and business * Control (management), an element of management * Control, an element of management accounting * Comptroller (or controller), a senior financial officer in an organization * Controllin ...

one. In many laboratory experiments it is good practice to have several replicate samples for the test being performed and have both a positive control and a

negative control A scientific control is an experiment or observation designed to minimize the effects of variables other than the independent variable (i.e. confounding variables). This increases the reliability of the results, often through a comparison be ...

. The results from replicate samples can often be averaged, or if one of the replicates is obviously inconsistent with the results from the other samples, it can be discarded as being the result of an experimental error (some step of the test procedure may have been mistakenly omitted for that sample). Most often, tests are done in duplicate or triplicate. A positive control is a procedure similar to the actual experimental test but is known from previous experience to give a positive result. A negative control is known to give a negative result. The positive control confirms that the basic conditions of the experiment were able to produce a positive result, even if none of the actual experimental samples produce a positive result. The negative control demonstrates the base-line result obtained when a test does not produce a measurable positive result. Most often the value of the negative control is treated as a "background" value to subtract from the test sample results. Sometimes the positive control takes the quadrant of a standard curve. An example that is often used in teaching laboratories is a controlled

protein Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, res ...

assay. Students might be given a fluid sample containing an unknown (to the student) amount of protein. It is their job to correctly perform a controlled experiment in which they determine the concentration of protein in the fluid sample (usually called the "unknown sample"). The teaching lab would be equipped with a protein standard solution with a known protein concentration. Students could make several positive control samples containing various dilutions of the protein standard. Negative control samples would contain all of the reagents for the protein assay but no protein. In this example, all samples are performed in duplicate. The assay is a colorimetric assay in which a spectrophotometer can measure the amount of protein in samples by detecting a colored complex formed by the interaction of protein molecules and molecules of an added dye. In the illustration, the results for the diluted test samples can be compared to the results of the standard curve (the blue line in the illustration) to estimate the amount of protein in the unknown sample. Controlled experiments can be performed when it is difficult to exactly control all the conditions in an experiment. In this case, the experiment begins by creating two or more sample groups that are ''probabilistically equivalent,'' which means that measurements of traits should be similar among the groups and that the groups should respond in the same manner if given the same treatment. This equivalency is determined by statistical methods that take into account the amount of variation between individuals and the

number A number is a mathematical object used to count, measure, and label. The original examples are the natural numbers 1, 2, 3, 4, and so forth. Numbers can be represented in language with number words. More universally, individual numbers c ...

of individuals in each group. In fields such as microbiology and chemistry, where there is very little variation between individuals and the group size is easily in the millions, these statistical methods are often bypassed and simply splitting a solution into equal parts is assumed to produce identical sample groups. Once equivalent groups have been formed, the experimenter tries to treat them identically except for the one ''variable'' that he or she wishes to isolate. Human experimentation requires special safeguards against outside variables such as the ''

placebo effect A placebo ( ) is a substance or treatment which is designed to have no therapeutic value. Common placebos include inert tablets (like sugar pills), inert injections (like Saline (medicine), saline), sham surgery, and other procedures. In general ...

''. Such experiments are generally ''

double blind In a blind or blinded experiment, information which may influence the participants of the experiment is withheld until after the experiment is complete. Good blinding can reduce or eliminate experimental biases that arise from a participants' expec ...

'', meaning that neither the volunteer nor the researcher knows which individuals are in the control group or the experimental group until after all of the data have been collected. This ensures that any effects on the volunteer are due to the treatment itself and are not a response to the knowledge that he is being treated. In human experiments, researchers may give a subject (person) a

stimulus A stimulus is something that causes a physiological response. It may refer to: *Stimulation **Stimulus (physiology), something external that influences an activity **Stimulus (psychology), a concept in behaviorism and perception *Stimulus (economi ...

that the subject responds to. The goal of the experiment is to measure the response to the stimulus by a

test method A test method is a method for a test in science or engineering, such as a physical test, chemical test, or statistical test. It is a definitive procedure that produces a test result. In order to ensure accurate and relevant test results, a test m ...

. In the

design of experiments The design of experiments (DOE, DOX, or experimental design) is the design of any task that aims to describe and explain the variation of information under conditions that are hypothesized to reflect the variation. The term is generally associ ...

, two or more "treatments" are applied to estimate the

difference Difference, The Difference, Differences or Differently may refer to: Music * ''Difference'' (album), by Dreamtale, 2005 * ''Differently'' (album), by Cassie Davis, 2009 ** "Differently" (song), by Cassie Davis, 2009 * ''The Difference'' (al ...

between the mean responses for the treatments. For example, an experiment on baking bread could estimate the difference in the responses associated with quantitative variables, such as the ratio of water to flour, and with qualitative variables, such as strains of yeast. Experimentation is the step in the

that helps people decide between two or more competing explanations—or hypotheses. These hypotheses suggest reasons to explain a phenomenon or predict the results of an action. An example might be the hypothesis that "if I release this ball, it will fall to the floor": this suggestion can then be tested by carrying out the experiment of letting go of the ball, and observing the results. Formally, a hypothesis is compared against its opposite or

null hypothesis In scientific research, the null hypothesis (often denoted ''H''0) is the claim that no difference or relationship exists between two sets of data or variables being analyzed. The null hypothesis is that any experimentally observed difference is d ...

("if I release this ball, it will not fall to the floor"). The null hypothesis is that there is no explanation or predictive power of the phenomenon through the reasoning that is being investigated. Once hypotheses are defined, an experiment can be carried out and the results analysed to confirm, refute, or define the accuracy of the hypotheses. Experiments can be also designed to estimate spillover effects onto nearby untreated units.

Natural experiments

The term "experiment" usually implies a controlled experiment, but sometimes controlled experiments are prohibitively difficult or impossible. In this case researchers resort to ''natural experiments'' or ''

quasi-experiments A quasi-experiment is an empirical interventional study used to estimate the causal impact of an intervention on target population without random assignment. Quasi-experimental research shares similarities with the traditional experimental design ...

.'' Natural experiments rely solely on observations of the variables of the system under study, rather than manipulation of just one or a few variables as occurs in controlled experiments. To the degree possible, they attempt to collect data for the system in such a way that contribution from all variables can be determined, and where the effects of variation in certain variables remain approximately constant so that the effects of other variables can be discerned. The degree to which this is possible depends on the observed correlation between explanatory variables in the observed data. When these variables are ''not'' well correlated, natural experiments can approach the power of controlled experiments. Usually, however, there is some correlation between these variables, which reduces the reliability of natural experiments relative to what could be concluded if a controlled experiment were performed. Also, because natural experiments usually take place in uncontrolled environments, variables from undetected sources are neither measured nor held constant, and these may produce illusory correlations in variables under study. Much research in several

science Science is a systematic endeavor that Scientific method, builds and organizes knowledge in the form of Testability, testable explanations and predictions about the universe. Science may be as old as the human species, and some of the earli ...

disciplines, including

economics Economics () is the social science that studies the production, distribution, and consumption of goods and services. Economics focuses on the behaviour and interactions of economic agents and how economies work. Microeconomics analyzes ...

human geography Human geography or anthropogeography is the branch of geography that studies spatial relationships between human communities, cultures, economies, and their interactions with the environment. It analyzes spatial interdependencies between social i ...

archaeology Archaeology or archeology is the scientific study of human activity through the recovery and analysis of material culture. The archaeological record consists of artifacts, architecture, biofacts or ecofacts, sites, and cultural landsca ...

sociology Sociology is a social science that focuses on society, human social behavior, patterns of social relationships, social interaction, and aspects of culture associated with everyday life. It uses various methods of empirical investigation an ...

, cultural anthropology,

geology Geology () is a branch of natural science concerned with Earth and other astronomical objects, the features or rocks of which it is composed, and the processes by which they change over time. Modern geology significantly overlaps all other Ea ...

paleontology Paleontology (), also spelled palaeontology or palæontology, is the scientific study of life that existed prior to, and sometimes including, the start of the Holocene epoch (roughly 11,700 years before present). It includes the study of fossi ...

ecology Ecology () is the study of the relationships between living organisms, including humans, and their physical environment. Ecology considers organisms at the individual, population, community, ecosystem, and biosphere level. Ecology overl ...

meteorology Meteorology is a branch of the atmospheric sciences (which include atmospheric chemistry and physics) with a major focus on weather forecasting. The study of meteorology dates back millennia, though significant progress in meteorology did no ...

, and

astronomy Astronomy () is a natural science that studies celestial objects and phenomena. It uses mathematics, physics, and chemistry in order to explain their origin and evolution. Objects of interest include planets, moons, stars, nebulae, g ...

, relies on quasi-experiments. For example, in astronomy it is clearly impossible, when testing the hypothesis "Stars are collapsed clouds of hydrogen", to start out with a giant cloud of hydrogen, and then perform the experiment of waiting a few billion years for it to form a star. However, by observing various clouds of hydrogen in various states of collapse, and other implications of the hypothesis (for example, the presence of various spectral emissions from the light of stars), we can collect data we require to support the hypothesis. An early example of this type of experiment was the first verification in the 17th century that light does not travel from place to place instantaneously, but instead has a measurable speed. Observation of the appearance of the moons of Jupiter were slightly delayed when Jupiter was farther from Earth, as opposed to when Jupiter was closer to Earth; and this phenomenon was used to demonstrate that the difference in the time of appearance of the moons was consistent with a measurable speed.

Field experiments

Field experiments are so named to distinguish them from

experiments, which enforce scientific control by testing a hypothesis in the artificial and highly controlled setting of a laboratory. Often used in the social sciences, and especially in economic analyses of education and health interventions, field experiments have the advantage that outcomes are observed in a natural setting rather than in a contrived laboratory environment. For this reason, field experiments are sometimes seen as having higher

external validity External validity is the validity of applying the conclusions of a scientific study outside the context of that study. In other words, it is the extent to which the results of a study can be generalized to and across other situations, people, stim ...

than laboratory experiments. However, like natural experiments, field experiments suffer from the possibility of contamination: experimental conditions can be controlled with more precision and certainty in the lab. Yet some phenomena (e.g., voter turnout in an election) cannot be easily studied in a laboratory.

Contrast with observational study

observational study In fields such as epidemiology, social sciences, psychology and statistics, an observational study draws inferences from a sample to a population where the independent variable is not under the control of the researcher because of ethical concer ...

is used when it is impractical, unethical, cost-prohibitive (or otherwise inefficient) to fit a physical or social system into a laboratory setting, to completely control confounding factors, or to apply random assignment. It can also be used when confounding factors are either limited or known well enough to analyze the data in light of them (though this may be rare when social phenomena are under examination). For an observational science to be valid, the experimenter must know and account for confounding factors. In these situations, observational studies have value because they often suggest hypotheses that can be tested with randomized experiments or by collecting fresh data. Fundamentally, however, observational studies are not experiments. By definition, observational studies lack the manipulation required for Baconian experiments. In addition, observational studies (e.g., in biological or social systems) often involve variables that are difficult to quantify or control. Observational studies are limited because they lack the statistical properties of randomized experiments. In a randomized experiment, the method of randomization specified in the experimental protocol guides the statistical analysis, which is usually specified also by the experimental protocol. Without a statistical model that reflects an objective randomization, the statistical analysis relies on a subjective model. Inferences from subjective models are unreliable in theory and practice. In fact, there are several cases where carefully conducted observational studies consistently give wrong results, that is, where the results of the observational studies are inconsistent and also differ from the results of experiments. For example, epidemiological studies of colon cancer consistently show beneficial correlations with broccoli consumption, while experiments find no benefit. A particular problem with observational studies involving human subjects is the great difficulty attaining fair comparisons between treatments (or exposures), because such studies are prone to selection bias, and groups receiving different treatments (exposures) may differ greatly according to their covariates (age, height, weight, medications, exercise, nutritional status, ethnicity, family medical history, etc.). In contrast, randomization implies that for each covariate, the mean for each group is expected to be the same. For any randomized trial, some variation from the mean is expected, of course, but the randomization ensures that the experimental groups have mean values that are close, due to the

central limit theorem In probability theory, the central limit theorem (CLT) establishes that, in many situations, when independent random variables are summed up, their properly normalized sum tends toward a normal distribution even if the original variables themsel ...

and

Markov's inequality In probability theory, Markov's inequality gives an upper bound for the probability that a non-negative function of a random variable is greater than or equal to some positive constant. It is named after the Russian mathematician Andrey Markov, ...

. With inadequate randomization or low sample size, the systematic variation in covariates between the treatment groups (or exposure groups) makes it difficult to separate the effect of the treatment (exposure) from the effects of the other covariates, most of which have not been measured. The mathematical models used to analyze such data must consider each differing covariate (if measured), and results are not meaningful if a covariate is neither randomized nor included in the model. To avoid conditions that render an experiment far less useful, physicians conducting medical trials—say for U.S.

Food and Drug Administration The United States Food and Drug Administration (FDA or US FDA) is a List of United States federal agencies, federal agency of the United States Department of Health and Human Services, Department of Health and Human Services. The FDA is respon ...

approval—quantify and randomize the covariates that can be identified. Researchers attempt to reduce the biases of observational studies with matching methods such as

propensity score matching In the statistical analysis of observational data, propensity score matching (PSM) is a statistical matching technique that attempts to estimate the effect of a treatment, policy, or other intervention by accounting for the covariates that predic ...

, which require large populations of subjects and extensive information on covariates. However, propensity score matching is no longer recommended as a technique because it can increase, rather than decrease, bias. Outcomes are also quantified when possible (bone density, the amount of some cell or substance in the blood, physical strength or endurance, etc.) and not based on a subject's or a professional observer's opinion. In this way, the design of an observational study can render the results more objective and therefore, more convincing.

Ethics

By placing the distribution of the independent variable(s) under the control of the researcher, an experiment—particularly when it involves human subjects—introduces potential ethical considerations, such as balancing benefit and harm, fairly distributing interventions (e.g., treatments for a disease), and informed consent. For example, in psychology or health care, it is unethical to provide a substandard treatment to patients. Therefore, ethical review boards are supposed to stop clinical trials and other experiments unless a new treatment is believed to offer benefits as good as current best practice. It is also generally unethical (and often illegal) to conduct randomized experiments on the effects of substandard or harmful treatments, such as the effects of ingesting arsenic on human health. To understand the effects of such exposures, scientists sometimes use observational studies to understand the effects of those factors. Even when experimental research does not directly involve human subjects, it may still present ethical concerns. For example, the nuclear bomb experiments conducted by the

Manhattan Project The Manhattan Project was a research and development undertaking during World War II that produced the first nuclear weapons. It was led by the United States with the support of the United Kingdom and Canada. From 1942 to 1946, the project w ...

implied the use of nuclear reactions to harm human beings even though the experiments did not directly involve any human subjects.

Notes

External links

*

Experiment in Physics
from Stanford Encyclopedia of Philosophy {{Authority control Research Design of experiments Causal inference