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Microscopic
The microscopic scale () is the scale of objects and events smaller than those that can easily be seen by the naked eye, requiring a lens or microscope to see them clearly. In physics, the microscopic scale is sometimes regarded as the scale between the macroscopic scale and the quantum scale. Microscopic units and measurements are used to classify and describe very small objects. One common microscopic length scale unit is the micrometre (also called a ''micron'') (symbol: μm), which is one millionth of a metre. History Whilst compound microscopes were first developed in the 1590s, the significance of the microscopic scale was only truly established in the 1600s when Marcello Malphigi and Antonie van Leeuwenhoek microscopically observed frog lungs and microorganisms. As microbiology was established, the significance of making scientific observations at a microscopic level increased. Published in 1665, Robert Hooke’s book Micrographia details his microscopic observat ...
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Antonie Van Leeuwenhoek
Antonie Philips van Leeuwenhoek ( ; ; 24 October 1632 – 26 August 1723) was a Dutch microbiologist and microscopist in the Golden Age of Dutch science and technology. A largely self-taught man in science, he is commonly known as " the Father of Microbiology", and one of the first microscopists and microbiologists. Van Leeuwenhoek is best known for his pioneering work in microscopy and for his contributions toward the establishment of microbiology as a scientific discipline. Raised in Delft, Dutch Republic, van Leeuwenhoek worked as a draper in his youth and founded his own shop in 1654. He became well recognized in municipal politics and developed an interest in lensmaking. In the 1670s, he started to explore microbial life with his microscope. This was one of the notable achievements of the Golden Age of Dutch exploration and discovery (). Using single-lensed microscopes of his own design and make, van Leeuwenhoek was the first to observe and to experiment with ...
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Optical Microscope
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope. In high-power microscopes, both eyepieces typically show the same image, but with a stereo microscope, slightly different images are used to create a 3-D effect. A camera is typically used to capture the image ( micrograph). The sample can be lit in a variety of ways. Transparent objects can be lit from below and solid objects can be lit with light coming through ( bright field) or around ( dark field) the objective lens. Po ...
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Algae
Algae (; singular alga ) is an informal term for a large and diverse group of photosynthetic eukaryotic organisms. It is a polyphyletic grouping that includes species from multiple distinct clades. Included organisms range from unicellular microalgae, such as ''Chlorella,'' ''Prototheca'' and the diatoms, to multicellular forms, such as the giant kelp, a large brown alga which may grow up to in length. Most are aquatic and autotrophic (they generate food internally) and lack many of the distinct cell and tissue types, such as stomata, xylem and phloem that are found in land plants. The largest and most complex marine algae are called seaweeds, while the most complex freshwater forms are the ''Charophyta'', a division of green algae which includes, for example, ''Spirogyra'' and stoneworts. No definition of algae is generally accepted. One definition is that algae "have chlorophyll ''a'' as their primary photosynthetic pigment and lack a sterile covering of cells around their re ...
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Statistical Mechanics
In physics, statistical mechanics is a mathematical framework that applies statistical methods and probability theory to large assemblies of microscopic entities. It does not assume or postulate any natural laws, but explains the macroscopic behavior of nature from the behavior of such ensembles. Statistical mechanics arose out of the development of classical thermodynamics, a field for which it was successful in explaining macroscopic physical properties—such as temperature, pressure, and heat capacity—in terms of microscopic parameters that fluctuate about average values and are characterized by probability distributions. This established the fields of statistical thermodynamics and statistical physics. The founding of the field of statistical mechanics is generally credited to three physicists: * Ludwig Boltzmann, who developed the fundamental interpretation of entropy in terms of a collection of microstates *James Clerk Maxwell, who developed models of probability di ...
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Microscope
A microscope () is a laboratory instrument used to examine objects that are too small to be seen by the naked eye. Microscopy is the science of investigating small objects and structures using a microscope. Microscopic means being invisible to the eye unless aided by a microscope. There are many types of microscopes, and they may be grouped in different ways. One way is to describe the method an instrument uses to interact with a sample and produce images, either by sending a beam of light or electrons through a sample in its optical path, by detecting photon emissions from a sample, or by scanning across and a short distance from the surface of a sample using a probe. The most common microscope (and the first to be invented) is the optical microscope, which uses lenses to refract visible light that passed through a thinly sectioned sample to produce an observable image. Other major types of microscopes are the fluorescence microscope, electron microscope (both the ...
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Objective (optics)
In optical engineering, the objective is the optical element that gathers light from the object being observed and focuses the light rays to produce a real image. Objectives can be a single lens or mirror, or combinations of several optical elements. They are used in microscopes, binoculars, telescopes, cameras, slide projectors, CD players and many other optical instruments. Objectives are also called object lenses, object glasses, or objective glasses. Microscope objectives The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes to a focus inside the microscope tube. The objective itself is usually a cylinder containing one or more lenses that are typically made of glass; its function is to collect light from the sample. Magnification One of the most important prope ...
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Macroscopic Scale
The macroscopic scale is the length scale on which objects or phenomena are large enough to be visible with the naked eye, without magnifying optical instruments. It is the opposite of microscopic. Overview When applied to physical phenomena and bodies, the macroscopic scale describes things as a person can directly perceive them, without the aid of magnifying devices. This is in contrast to observations (microscopy) or theories ( microphysics, statistical physics) of objects of geometric lengths smaller than perhaps some hundreds of micrometers. A macroscopic view of a ball is just that: a ball. A microscopic view could reveal a thick round skin seemingly composed entirely of puckered cracks and fissures (as viewed through a microscope) or, further down in scale, a collection of molecules in a roughly spherical shape (as viewed through an electron microscope). An example of a physical theory that takes a deliberately macroscopic viewpoint is thermodynamics. An example of a top ...
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Transmission Electron Microscopy
Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The image is then magnified and focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a sensor such as a scintillator attached to a charge-coupled device. Transmission electron microscopes are capable of imaging at a significantly higher resolution than light microscopes, owing to the smaller de Broglie wavelength of electrons. This enables the instrument to capture fine detail—even as small as a single column of atoms, which is thousands of times smaller than a resolvable object seen in a light microscope. Transmission electron microscopy is a major analytical me ...
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Volvox
''Volvox'' is a polyphyletic genus of chlorophyte green algae in the family Volvocaceae. It forms spherical colonies of up to 50,000 cells. They live in a variety of freshwater habitats, and were first reported by Antonie van Leeuwenhoek in 1700. ''Volvox'' diverged from unicellular ancestors approximately . Description ''Volvox'' is a polyphyletic genus in the volvocine green algae clade. Each mature ''Volvox'' colony is composed of up to thousands of cells from two differentiated cell types: numerous flagellate somatic cells and a smaller number of germ cells lacking in soma that are embedded in the surface of a hollow sphere or coenobium containing an extracellular matrix made of glycoproteins. Adult somatic cells comprise a single layer with the flagella facing outward. The cells swim in a coordinated fashion, with distinct anterior and posterior poles. The cells have anterior eyespots that enable the colony to swim toward light. The cells of colonies in the more ba ...
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Cladocera
The Diplostraca or Cladocera, commonly known as water fleas, are a superorder of small crustaceans that feed on microscopic chunks of organic matter (excluding some predatory forms). Over 1000 species have been recognised so far, with many more undescribed. The oldest fossils of diplostracans date to the Jurassic, though their modern morphology suggests that they originated substantially earlier, during the Paleozoic. Some have also adapted to a life in the ocean, the only members of Branchiopoda to do so, even if several anostracans live in hypersaline lakes. Most are long, with a down-turned head with a single median compound eye, and a carapace covering the apparently unsegmented thorax and abdomen. Most species show cyclical parthenogenesis, where asexual reproduction is occasionally supplemented by sexual reproduction, which produces resting eggs that allow the species to survive harsh conditions and disperse to distant habitats. Description They are mostly long, with t ...
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Stentor (protozoa)
''Stentor'', sometimes called trumpet animalcules, are a genus of filter-feeding, heterotrophic ciliates, representative of the heterotrichs. They are usually horn-shaped, and reach lengths of two millimeters; as such, they are among the largest known extant unicellular organisms. They reproduce asexually through binary fission. Description The body, or cortex, is generally horn-shaped, hence the association with the Greek herald, and the former name "trumpet animalcule". A ring of prominent cilia around the anterior "bell" sweep in food and aid in swimming. Some reach several millimeters in length, making them among the largest single-celled organisms. ''Stentor'' can come in different colors: for example, ''S. coeruleus'' can appear blue due to the presence of stentorin, a natural pigment. As in many freshwater protozoans, ''Stentor'' has a contractile vacuole. Because the concentration of salt inside the cell and in the surrounding freshwater is different, ''Stentor'' mu ...
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Naked Eye
Naked eye, also called bare eye or unaided eye, is the practice of engaging in visual perception unaided by a magnifying, light-collecting optical instrument, such as a telescope or microscope, or eye protection. Vision corrected to normal acuity using corrective lenses is still considered "naked". In astronomy, the naked eye may be used to observe celestial events and objects visible without equipment, such as conjunctions, passing comets, meteor showers, and the brightest asteroids, including 4 Vesta. Sky lore and various tests demonstrate an impressive variety of phenomena visible to the unaided eye. Basic properties Some basic properties of the human eye are: *Quick autofocus from distances of 25 cm (young people) to 50 cm (most people 50 years and older) to infinity. * Angular resolution: about 1  arcminute, approximately 0.017° or 0.0003 radians, which corresponds to 0.3 m at a 1 km distance. * Field of view (FOV): simultaneous visua ...
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