HOME

TheInfoList



Click Here for Items Related To - resolution (chromatography)
OR:
resolution (chromatography) on:   [Wikipedia]   [Google]   [Amazon]

In
chromatography In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system ...
, resolution is a measure of the separation of two peaks of different retention time ''t'' in a
chromatogram In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system ...
.


Expression

Chromatographic peak resolution is given by :R_s = 2\cfrac where tR is the retention time and wb is the peak width at baseline. Here compound 1 elutes before compound 2. If the peaks have the same width :R_s = \cfrac .


Plate number

The
theoretical plate A theoretical plate in many separation processes is a hypothetical zone or stage in which two phases, such as the liquid and vapor phases of a substance, establish an equilibrium with each other. Such equilibrium stages may also be referred to as a ...
height is given by :H = \frac where L is the column length and N the number of theoretical plates. The relation between plate number and peak width at the base is given by :N = 16 \cdot \left(\frac\right)^2 \,.


See also

* Van Deemter equation *
Resolution (mass spectrometry) In mass spectrometry, resolution is a measure of the ability to distinguish two peaks of slightly different mass-to-charge ratios ''ΔM'', in a mass spectrum. Resolution and resolving power There are two different definitions of resolution and ...
*
Image resolution Image resolution is the detail an image holds. The term applies to digital images, film images, and other types of images. "Higher resolution" means more image detail. Image resolution can be measured in various ways. Resolution quantifies how cl ...


References


External links


IUPAC Nomenclature for Chromatography
{{chromatography,