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A micrograph or photomicrograph is a photograph or digital image taken through a
microscope A microscope () is a laboratory instrument used to examine objects that are too small to be seen by the naked eye. Microscopy is the science of investigating small objects and structures using a microscope. Microscopic means being invisi ...
or similar device to show a magnified image of an object. This is opposed to a macrograph or photomacrograph, an image which is also taken on a microscope but is only slightly magnified, usually less than 10 times. Micrography is the practice or art of using microscopes to make photographs. A micrograph contains extensive details of microstructure. A wealth of information can be obtained from a simple micrograph like behavior of the material under different conditions, the phases found in the system, failure analysis, grain size estimation, elemental analysis and so on. Micrographs are widely used in all fields of microscopy.


Types


Photomicrograph

A light micrograph or photomicrograph is a micrograph prepared using an optical microscope, a process referred to as ''photomicroscopy''. At a basic level, photomicroscopy may be performed simply by connecting a camera to a microscope, thereby enabling the user to take photographs at reasonably high magnification. Scientific use began in England in 1850 by Prof Richard Hill Norris FRSE for his studies of blood cells. Roman Vishniac was a pioneer in the field of photomicroscopy, specializing in the photography of living creatures in full motion. He also made major developments in light-interruption photography and color photomicroscopy. Photomicrographs may also be obtained using a USB microscope attached directly to a home computer or laptop.


Electron micrograph

An electron micrograph is a micrograph prepared using an electron microscope.


Magnification and micron bars

Micrographs usually have micron bars, or magnification ratios, or both. Magnification is a ratio between the size of an object on a picture and its real size. Unfortunately, magnification can be a misleading parameter as it depends on the final size of a printed picture and therefore varies with picture size. A ''scale bar'', or ''micron bar'', is a line of known length displayed on a picture. The bar can be used for measurements on a picture. When the picture is resized the bar is also resized making it possible to recalculate the magnification. Ideally, all pictures destined for publication/presentation should be supplied with a scale bar; the magnification ratio is optional. All but one (limestone) of the micrographs presented on this page do not have a micron bar; supplied magnification ratios are likely incorrect, as they were not calculated for pictures at the present size.


Micrography as art

The microscope has been mainly used for scientific discovery. It has also been linked to the arts since its invention in the 17th century. Early adopters of the microscope, such as Robert Hooke and Antonie van Leeuwenhoek, were excellent illustrators.
Cornelius Varley Cornelius Varley, FRSA (21 November 1781 – 2 October 1873) was a British water-colour painter and optical instrument-maker. He invented the graphic telescope and the graphic microscope. Biography Varley was born at Hackney, then a village ...
's graphic microscope made sketching from a microscope easier with a camera-lucida-like mechanism. After the invention of
photography Photography is the art, application, and practice of creating durable images by recording light, either electronically by means of an image sensor, or chemically by means of a light-sensitive material such as photographic film. It is employe ...
in the 1820s the microscope was later combined with the camera to take pictures instead of relying on an artistic rendering. Since the early 1970s individuals have been using the microscope as an artistic instrument. Websites and traveling art exhibits such as the Nikon Small World and Olympus Bioscapes have featured a range of images for the sole purpose of artistic enjoyment. Some collaborative groups, such as the Paper Project have also incorporated microscopic imagery into tactile art pieces as well as 3D immersive rooms and dance performances. In 2015, photographer and gemologist Danny J. Sanchez photographed mineral and gemstone interiors in works referred to as "otherworldly."


Photomicrography in smartphones

Bellina and Missoni first published a paper in 2009 describing the method of photomicrography in a smartphone with free-hand technique. An operator just need to focus her/his smartphone camera on the eyepiece of a microscope and capture the photo. However, later, various commercial and home-made adapters were introduced to ease focusing. A home-made adapter was also made using scrap materials and a Coca-cola aluminum can.


Gallery

Image:Colpoda400xm2.jpg, Measurements of a large '' Colpodium'' at 400x. Image:Amoeba400XM.jpg, Measurements of a large
amoeba An amoeba (; less commonly spelled ameba or amœba; plural ''am(o)ebas'' or ''am(o)ebae'' ), often called an amoeboid, is a type of cell or unicellular organism with the ability to alter its shape, primarily by extending and retracting pseudop ...
at 400x. File:Wilson A. Bentley snowflake, 1890.jpg, Snowflake micrograph by Wilson Bentley, 1890. File:Misc pollen.jpg, An image of pollen taken from a scanning electron microscope.


See also

* Close-up * Digital microscope * Macro photography *
Microphotograph Microphotographs are photographs shrunk to microscopic scale.
* Microscopy * USB microscope


References


External links


Shots with a Microscope
– a basic, comprehensive guide to photomicrography
Scientific photomicrographs
– free scientific quality photomicrographs by Doc. RNDr. Josef Reischig, CSc.
Seeing Beyond the Human Eye
Video produced by
Off Book (web series) ''Off Book'' is a web series on digital culture and art created for PBS by Kornhaber Brown, a Webby award-winning production studio that creates web series, videos, and motion graphics. The series has been viewed more than six million times, an ...

Solomon C. Fuller bio



Photomicrography by Danny J. Sanchez

Dennis Kunkel Microscopy

Andrew Paul Leonard, APL Microscopic

Cell Centered Database - Montage

Nikon Small World

Olympus Bioscapes


{{Authority control Photographic techniques Microscopy