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A biological hazard, or biohazard, is a
biological Biology is the scientific study of life. It is a natural science with a broad scope but has several unifying themes that tie it together as a single, coherent field. For instance, all organisms are made up of cells that process hereditary i ...
substance that poses a threat to the health of
living organisms In biology, an organism () is any living system that functions as an individual entity. All organisms are composed of cells (cell theory). Organisms are classified by taxonomy into groups such as multicellular animals, plants, and fungi ...
, primarily humans. This could include a sample of a
microorganism A microorganism, or microbe,, ''mikros'', "small") and ''organism'' from the el, ὀργανισμός, ''organismós'', "organism"). It is usually written as a single word but is sometimes hyphenated (''micro-organism''), especially in olde ...
,
virus A virus is a submicroscopic infectious agent that replicates only inside the living cells of an organism. Viruses infect all life forms, from animals and plants to microorganisms, including bacteria and archaea. Since Dmitri Ivanovsky' ...
or
toxin A toxin is a naturally occurring organic poison produced by metabolic activities of living cells or organisms. Toxins occur especially as a protein or conjugated protein. The term toxin was first used by organic chemist Ludwig Brieger (1849� ...
that can adversely affect human
health Health, according to the World Health Organization, is "a state of complete physical, mental and social well-being and not merely the absence of disease and infirmity". World Health Organization. (2006)''Constitution of the World Health Org ...
. A biohazard could also be a substance harmful to other animals. The term and its associated symbol are generally used as a warning, so that those potentially exposed to the substances will know to take precautions. The biohazard symbol was developed in 1966 by Charles Baldwin, an environmental-health engineer working for the
Dow Chemical Company The Dow Chemical Company, officially Dow Inc., is an American multinational chemical corporation headquartered in Midland, Michigan, United States. The company is among the three largest chemical producers in the world. Dow manufactures plastics ...
on the containment products. It is used in the labeling of biological materials that carry a significant health risk, including viral samples and used hypodermic needles. In
Unicode Unicode, formally The Unicode Standard,The formal version reference is is an information technology standard for the consistent encoding, representation, and handling of text expressed in most of the world's writing systems. The standard, whi ...
, the
biohazard symbol Hazard symbols or warning symbols are recognisable symbols designed to warn about hazardous or dangerous materials, locations, or objects, including electric currents, poisons, and radioactivity. The use of hazard symbols is often regulated by ...
is U+2623 ().


ANSI Z535/OSHA/ISO regulation

Biohazardous safety issues are identified with specified labels, signs and paragraphs established by the American National Standards Institute (ANSI). Today,
ANSI Z535 ANSI Z535 is an American standard that sets forth a system for presenting safety and accident prevention information. It corresponds to the international ISO 3864 standard. Sub-Standards The ANSI Z535 standard comprises the following six indi ...
standards for biohazards are used worldwide and should always be used appropriately within
ANSI Z535 ANSI Z535 is an American standard that sets forth a system for presenting safety and accident prevention information. It corresponds to the international ISO 3864 standard. Sub-Standards The ANSI Z535 standard comprises the following six indi ...
Hazardous Communications (HazCom) signage, labeling and paragraphs. The goal is to help workers rapidly identify the severity of a biohazard from a distance and through colour and design standardization. Biological hazard symbol design: * A red on white or white-coloured background is used behind a black biohazard symbol when integrated with a DANGER sign, label or paragraph. * An orange on black or white-coloured background is used behind a black biohazard symbol when integrated with a WARNING sign, label or paragraph. * A yellow on black or white-coloured background is used behind a black biohazard symbol when integrated with a CAUTION sign, label or paragraph. * A green on white or white-coloured background is used behind a black biohazard symbol when integrated with a NOTICE sign, label or paragraph. DANGER is used to identify a biohazard that will cause death. WARNING is used to identify a biohazard that may cause death. CAUTION is used to identify a biohazard that will cause injury, but not death. NOTICE is used to identify a non-injury biohazard message (e.g. hygiene, cleanup or general lab policies). OSHA requires the use of proper ANSI HazCom where applicable in American workplaces. States and local governments also use these standards as codes and laws within their own jurisdictions. Proper use of ANSI Z535 signs, labels and paragraphs are written into many of OSHA's standards for HazCom and crafted to integrate with ISO symbols. Reference
ANSI Z535 ANSI Z535 is an American standard that sets forth a system for presenting safety and accident prevention information. It corresponds to the international ISO 3864 standard. Sub-Standards The ANSI Z535 standard comprises the following six indi ...
for a complete description on how to use DANGER, WARNING, CAUTION and NOTICE signs, labels or paragraphs.


UN/ISO Classification

Biohazardous agents are classified for transportation by
UN number A UN number (United Nations number) is a four-digit number that identifies hazardous materials, and articles (such as explosives, flammable liquids, oxidizers, toxic liquids, etc.) in the framework of international trade and transport. Some haza ...
: * Category A, UN 2814 – Infectious substance, affecting humans: An infectious substance in a form capable of causing permanent disability or life-threatening or fatal disease in otherwise healthy humans or animals when exposure to it occurs. * Category A, UN 2900 – Infectious substance, affecting animals (only): An infectious substance that is not in a form generally capable of causing permanent disability or life-threatening or fatal disease in otherwise healthy humans and animals when exposure to themselves occurs. * Category B, UN 3373 – Biological substance transported for diagnostic or investigative purposes. * Regulated Medical Waste, UN 3291 – Waste or reusable material derived from medical treatment of an animal or human, or from biomedical research, which includes the production and testing.


Levels of biohazard

The United States
Centers for Disease Control and Prevention The Centers for Disease Control and Prevention (CDC) is the national public health agency of the United States. It is a United States federal agency, under the Department of Health and Human Services, and is headquartered in Atlanta, Georg ...
(CDC) categorizes various diseases in levels of biohazard, Level 1 being minimum risk and Level 4 being extreme risk. Laboratories and other facilities are categorized as BSL ( Biosafety Level) 1–4 or as ''P1'' through ''P4'' for short (Pathogen or Protection Level). * Biohazard Level 1: Bacteria and viruses including ''
Bacillus subtilis ''Bacillus subtilis'', known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants, humans and marine sponges. As a member of the genus '' Bacillus ...
'', canine
hepatitis Hepatitis is inflammation of the liver tissue. Some people or animals with hepatitis have no symptoms, whereas others develop yellow discoloration of the skin and whites of the eyes ( jaundice), poor appetite, vomiting, tiredness, abdominal p ...
, ''
Escherichia coli ''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Esc ...
'', and varicella (
chickenpox Chickenpox, also known as varicella, is a highly contagious disease caused by the initial infection with varicella zoster virus (VZV). The disease results in a characteristic skin rash that forms small, itchy blisters, which eventually scab ...
), as well as some cell cultures and non-infectious bacteria. At this level precautions against the biohazardous materials in question are minimal, most likely involving gloves and some sort of facial protection. * Biohazard Level 2: Bacteria and viruses that cause only mild disease to humans, or are difficult to contract via
aerosol An aerosol is a suspension of fine solid particles or liquid droplets in air or another gas. Aerosols can be natural or anthropogenic. Examples of natural aerosols are fog or mist, dust, forest exudates, and geyser steam. Examples of anthropo ...
in a lab setting, such as hepatitis A, B, and C, some
influenza A '' A virus'' (''IAV'') causes influenza in birds and some mammals, and is the only species of the genus ''Alphainfluenzavirus'' of the virus family ''Orthomyxoviridae''. Strains of all subtypes of influenza A virus have been isolated from wild ...
strains,
Human respiratory syncytial virus Respiratory syncytial virus (RSV), also called human respiratory syncytial virus (hRSV) and human orthopneumovirus, is a common, contagious virus that causes infections of the respiratory tract. It is a negative-sense, single-stranded RNA virus ...
,
Lyme disease Lyme disease, also known as Lyme borreliosis, is a vector-borne disease caused by the ''Borrelia'' bacterium, which is spread by ticks in the genus '' Ixodes''. The most common sign of infection is an expanding red rash, known as erythema mig ...
,
salmonella ''Salmonella'' is a genus of rod-shaped (bacillus) Gram-negative bacteria of the family Enterobacteriaceae. The two species of ''Salmonella'' are ''Salmonella enterica'' and ''Salmonella bongori''. ''S. enterica'' is the type species and is f ...
,
mumps MUMPS ("Massachusetts General Hospital Utility Multi-Programming System"), or M, is an imperative, high-level programming language with an integrated transaction processing key–value database. It was originally developed at Massachusetts Gener ...
,
measles Measles is a highly contagious infectious disease caused by measles virus. Symptoms usually develop 10–12 days after exposure to an infected person and last 7–10 days. Initial symptoms typically include fever, often greater than , cough ...
,
scrapie Scrapie () is a fatal, degenerative disease affecting the nervous systems of sheep and goats. It is one of several transmissible spongiform encephalopathies (TSEs), and as such it is thought to be caused by a prion. Scrapie has been known since ...
,
dengue fever Dengue fever is a mosquito-borne tropical disease caused by the dengue virus. Symptoms typically begin three to fourteen days after infection. These may include a high fever, headache, vomiting, muscle and joint pains, and a characteristic ...
, and HIV. Routine diagnostic work with clinical specimens can be done safely at Biosafety Level 2, using Biosafety Level 2 practices and procedures. Research work (including co-cultivation, virus replication studies, or manipulations involving concentrated virus) can be done in a BSL-2 (P2) facility, using BSL-3 practices and procedures. * Biohazard Level 3: Bacteria and viruses that can cause severe to fatal disease in humans, but for which vaccines or other treatments exist, such as
anthrax Anthrax is an infection caused by the bacterium '' Bacillus anthracis''. It can occur in four forms: skin, lungs, intestinal, and injection. Symptom onset occurs between one day and more than two months after the infection is contracted. The s ...
,
West Nile virus West Nile virus (WNV) is a single-stranded RNA virus that causes West Nile fever. It is a member of the family '' Flaviviridae'', from the genus '' Flavivirus'', which also contains the Zika virus, dengue virus, and yellow fever virus. The vir ...
, Venezuelan equine encephalitis,
SARS coronavirus Severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1; or Severe acute respiratory syndrome coronavirus, SARS-CoV) is a strain of coronavirus that causes severe acute respiratory syndrome (SARS), the respiratory illness responsible for t ...
,
MERS coronavirus ''Middle East respiratory syndrome–related coronavirus'' (''MERS-CoV''), or EMC/2012 ( HCoV-EMC/2012), is the virus that causes Middle East respiratory syndrome (MERS). It is a species of coronavirus which infects humans, bats, and camels. Th ...
,
SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) is a strain of coronavirus that causes COVID-19 (coronavirus disease 2019), the respiratory illness responsible for the ongoing COVID-19 pandemic. The virus previously had a ...
, Influenza A
H5N1 Influenza A virus subtype H5N1 (A/H5N1) is a subtype of the influenza A virus which can cause illness in humans and many other animal species. A bird-adapted strain of H5N1, called HPAI A(H5N1) for highly pathogenic avian influenza virus of type ...
,
hantaviruses ''Orthohantavirus'' is a genus of single-stranded, enveloped, negative-sense RNA viruses in the family '' Hantaviridae'' within the order ''Bunyavirales''. Members of this genus may be called orthohantaviruses or simply hantaviruses. Orthohanta ...
,
tuberculosis Tuberculosis (TB) is an infectious disease usually caused by ''Mycobacterium tuberculosis'' (MTB) bacteria. Tuberculosis generally affects the lungs, but it can also affect other parts of the body. Most infections show no symptoms, in w ...
,
typhus Typhus, also known as typhus fever, is a group of infectious diseases that include epidemic typhus, scrub typhus, and murine typhus. Common symptoms include fever, headache, and a rash. Typically these begin one to two weeks after exposure. ...
, Rift Valley fever,
Rocky Mountain spotted fever Rocky Mountain spotted fever (RMSF) is a bacterial disease spread by ticks. It typically begins with a fever and headache, which is followed a few days later with the development of a rash. The rash is generally made up of small spots of bleedin ...
,
yellow fever Yellow fever is a viral disease of typically short duration. In most cases, symptoms include fever, chills, loss of appetite, nausea, muscle pains – particularly in the back – and headaches. Symptoms typically improve within five days. In ...
, and
malaria Malaria is a mosquito-borne infectious disease that affects humans and other animals. Malaria causes symptoms that typically include fever, tiredness, vomiting, and headaches. In severe cases, it can cause jaundice, seizures, coma, or deat ...
. * Biohazard Level 4: Viruses that cause severe to fatal disease in humans, and for which vaccines or other treatments are ''not'' available, such as Bolivian hemorrhagic fever,
Marburg virus Marburg virus (MARV) is a hemorrhagic fever virus of the '' Filoviridae'' family of viruses and a member of the species ''Marburg marburgvirus'', genus '' Marburgvirus''. It causes Marburg virus disease in primates, a form of viral hemorrhagi ...
,
Ebola virus ''Zaire ebolavirus'', more commonly known as Ebola virus (; EBOV), is one of six known species within the genus ''Ebolavirus''. Four of the six known ebolaviruses, including EBOV, cause a severe and often fatal hemorrhagic fever in humans and o ...
, Lassa fever virus,
Crimean–Congo hemorrhagic fever Crimean–Congo hemorrhagic fever (CCHF) is a viral disease. Symptoms of CCHF may include fever, muscle pains, headache, vomiting, diarrhea, and bleeding into the skin. Onset of symptoms is less than two weeks following exposure. Complications ...
, and other
hemorrhagic Bleeding, hemorrhage, haemorrhage or blood loss, is blood escaping from the circulatory system from damaged blood vessels. Bleeding can occur internally, or externally either through a natural opening such as the mouth, nose, ear, urethra, ...
diseases, as well as
Nipah virus Nipah virus, scientific name ''Nipah henipavirus'', is a bat-borne virus that causes Nipah virus infection in humans and other animals, a disease with a high mortality rate. Numerous disease outbreaks caused by Nipah virus have occurred in Sou ...
.
Variola virus Smallpox was an infectious disease caused by variola virus (often called smallpox virus) which belongs to the genus Orthopoxvirus. The last naturally occurring case was diagnosed in October 1977, and the World Health Organization (WHO) cer ...
(
smallpox Smallpox was an infectious disease caused by variola virus (often called smallpox virus) which belongs to the genus Orthopoxvirus. The last naturally occurring case was diagnosed in October 1977, and the World Health Organization (WHO) c ...
) is an agent that is worked with at BSL-4 despite the existence of a vaccine, as it has been eradicated and thus the general population is no longer routinely vaccinated. When dealing with biological hazards at this level, the use of a
positive pressure personnel suit Positive pressure personnel suits (PPPS)—or positive pressure protective suits, informally known as "space suits", "moon suits", "blue suits", etc.—are highly specialized, totally encapsulating, industrial protection garments worn only with ...
with a segregated air supply is mandatory. The entrance and exit of a Level Four biolab will contain multiple showers, a vacuum room, an
ultraviolet Ultraviolet (UV) is a form of electromagnetic radiation with wavelength from 10 nm (with a corresponding frequency around 30  PHz) to 400 nm (750  THz), shorter than that of visible light, but longer than X-rays. UV radiatio ...
light room, autonomous detection system, and other safety precautions designed to destroy all traces of the biohazard. Multiple airlocks are employed and are electronically secured to prevent doors from both opening at the same time. All air and water service going to and coming from a Biosafety Level 4 (P4) lab will undergo similar decontamination procedures to eliminate the possibility of an accidental release. Currently there are no bacteria classified at this level.


See also

* * * * * * * * * List of laboratory biosecurity incidents * * * * * *


Notes


References


Bibliography

* * *


External links


"Biosafety in Microbiological and Biomedical Laboratories"
official CDC guide.
"Prevention of Biological Hazards"
EU-OSHA The European Agency for Safety and Health at Work (EU-OSHA) is a decentralised agency of the European Union with the task of collecting, analysing and disseminating relevant information that can serve the needs of people involved in safety and h ...

"Symbol Making"
an account of the development of the symbol in 1966.
"BioSeal Systems"
Biosafety level 4 containment.
Biohazard Remediation Compliance Overview

"Example of Reclassified Biohazard to Level 4"
article from The Lancet concerning the reclassification of Ebola after observation.
"Biological hazards related to working alone safety"
an article focusing on working alone safety when dealing with biological hazards {{DEFAULTSORT:Biological Hazard * Safety Security Life sciences industry Occupational hazards