Karyotype of the T47D breast cancer cell line

T-47D is a human

breast cancer Breast cancer is a cancer that develops from breast tissue. Signs of breast cancer may include a Breast lump, lump in the breast, a change in breast shape, dimpling of the skin, Milk-rejection sign, milk rejection, fluid coming from the nipp ...

cell line An immortalised cell line is a population of cells from a multicellular organism that would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells ...

commonly used in biomedical research involving the

hormonal A hormone (from the Greek participle , "setting in motion") is a class of signaling molecules in multicellular organisms that are sent to distant organs or tissues by complex biological processes to regulate physiology and behavior. Hormones a ...

expression of cancer cells.

Characteristics

The cells were derived from the

pleural effusion A pleural effusion is accumulation of excessive fluid in the pleural space, the potential space that surrounds each lung. Under normal conditions, pleural fluid is secreted by the parietal pleural capillaries at a rate of 0.6 millilitre per kilog ...

of a ductal carcinoma found in the

mammary gland A mammary gland is an exocrine gland that produces milk in humans and other mammals. Mammals get their name from the Latin word ''mamma'', "breast". The mammary glands are arranged in organs such as the breasts in primates (for example, human ...

of an elderly human patient. T-47D cells are distinct from other human breast cancer cells in that their progesterone receptors are not regulated by

estradiol Estradiol (E2), also called oestrogen, oestradiol, is an estrogen steroid hormone and the major female sex hormone. It is involved in the regulation of female reproductive cycles such as estrous and menstrual cycles. Estradiol is responsible ...

, a hormone that is abundant within the cells themselves.

Usage

T-47D cells have been employed in studies of the effects of

progesterone Progesterone (; P4) is an endogenous steroid and progestogen sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. It belongs to a group of steroid hormones called the progestogens and is the ma ...

on breast cancer and the corresponding transcriptional regulation caused by introduced drugs. T-47D cells can be implanted into immunodeficient mice, forming xenografts that can undergo drug testing with corresponding receptor status and

angiogenesis Angiogenesis is the physiological process through which new blood vessels form from pre-existing vessels, formed in the earlier stage of vasculogenesis. Angiogenesis continues the growth of the vasculature mainly by processes of sprouting and ...

changes. The cells have been noted to be extremely resistant to

estrogens Estrogen (also spelled oestrogen in British English; see spelling differences) is a category of sex hormone responsible for the development and regulation of the female reproductive system and secondary sex characteristics. There are three ma ...

and antiestrogens, possibly due to inhibition by 5-bromodeoxyuridine and sodium butyrate.

References

Human cell lines

External links

Cellosaurus entry for T-47D
{{oncology-stub