Sulforhodamine B or Kiton Red 620 (
C27 H30 N2 O7 S2) is a
fluorescent
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, ...
dye with uses spanning from
laser-induced fluorescence (LIF) to the quantification of
cellular protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, res ...
s of
cultured cells. This
red
Red is the color at the long wavelength end of the visible spectrum of light, next to orange and opposite violet. It has a dominant wavelength of approximately 625–740 nanometres. It is a primary color in the RGB color model and a secondar ...
solid dye is very
water-soluble
In chemistry, solubility is the ability of a substance, the solute, to form a solution with another substance, the solvent. Insolubility is the opposite property, the inability of the solute to form such a solution.
The extent of the solub ...
.
Spectroscopy
The dye has maximal absorbance at 565
nm light
Light or visible light is electromagnetic radiation that can be perceived by the human eye. Visible light is usually defined as having wavelengths in the range of 400–700 nanometres (nm), corresponding to frequencies of 750–420 te ...
and maximal fluorescence emission at 586 nm light.
It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.
Applications
Sulforhodamine B is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (
cytotoxicity assay).
References
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Benzenesulfonates
Rhodamine dyes
Xanthenes
Diethylamino compounds