The selector technique is a method to amplify and

multiplex Multiplex may refer to: Science and technology * Multiplex communication, combining many signals into one transmission circuit or channel ** Multiplex (television), a group of digital television or radio channels that are combined for broadcast * ...

genomic DNA.

Process

Genomic

DNA Deoxyribonucleic acid (; DNA) is a polymer composed of two polynucleotide chains that coil around each other to form a double helix. The polymer carries genetic instructions for the development, functioning, growth and reproduction of al ...

is digested with

restriction enzymes A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class o ...

, circularized by hybridisation to selectors and subsequently attached to a

vector Vector most often refers to: * Euclidean vector, a quantity with a magnitude and a direction * Disease vector, an agent that carries and transmits an infectious pathogen into another living organism Vector may also refer to: Mathematics a ...

sequence by ligation. The procedure results in circular DNA

molecules A molecule is a group of two or more atoms that are held together by attractive forces known as chemical bonds; depending on context, the term may or may not include ions that satisfy this criterion. In quantum physics, organic chemistry ...

with an included general

primer Primer may refer to: Arts, entertainment, and media Films * ''Primer'' (film), a 2004 feature film written and directed by Shane Carruth * ''Primer'' (video), a documentary about the funk band Living Colour Literature * Primer (textbook), a te ...

pair motif that can be used for amplification by PCR or

RCA RCA Corporation was a major American electronics company, which was founded in 1919 as the Radio Corporation of America. It was initially a patent pool, patent trust owned by General Electric (GE), Westinghouse Electric Corporation, Westinghou ...

Selector construct

A selector consists of two oligonucleotides, one Vector oligonucleotide and one Selector probe. Together they form one Selector with target specific ends on each side of a general primer motif.

Selection mechanisms

# A selector probe hybridizes with both ends of the selected target. # A selector probe hybridizes with one end to the 3’ end of the target and the other end to an internal sequence of the target. The protruding 5' end is cleaved off using

Taq polymerase ''Taq'' polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism ''Thermus aquaticus,'' from which it was originally isolated by master's student Alice Chien et al. in 1976. Its name is often abbr ...

Publications

Demonstration of the selector methodThe PieceMaker software for designing selector experiments
{{DEFAULTSORT:Selector-Technique DNA