List Of EC Numbers (EC 3)

This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology. All official information is tabulated at the website of the committee. The database is developed and maintained by Andrew McDonald.

EC 3.1: Acting on Ester Bonds

EC 3.1.1: Carboxylic Ester Hydrolases

* : carboxylesterase
* : arylesterase
* : triacylglycerol lipase
* : phospholipase A₂
* : lysophospholipase
* : acetylesterase
* : acetylcholinesterase
* : cholinesterase
* EC 3.1.1.9: deleted, a side reaction of cholinesterase
* : tropinesterase
* : pectinesterase
* EC 3.1.1.12: deleted, identical with carboxylesterase
* : sterol esterase
* : chlorophyllase
* : L-arabinonolactonase
* EC 3.1.1.16: deleted, mixture of (muconolactone Δ-isomerase) and (3-oxoadipate enol-lactonase)
* : gluconolactonase
* EC 3.1.1.18: deleted, now included with gluconolactonase
* : uronolactonase
* : tannase
* EC 3.1.1.21: deleted, now known to be catalysed by , carboxylesterase and , triacylglycerol lipase.
* : hydroxybutyrate-dimer hydrolase
* : acylglycerol lipase
* : 3-oxoadipate enol-lactonase
* : 1,4-lactonase
* : galactolipase
* : 4-pyridoxolactonase
* : acylcarnitine hydrolase
* : aminoacyl-tRNA hydrolase
* : D-arabinonolactonase
* : 6-phosphogluconolactonase
* : phospholipase A₁
* : 6-acetylglucose deacetylase
* : lipoprotein lipase
* : dihydrocoumarin hydrolase
* : limonin-D-ring-lactonase
* : steroid-lactonase
* : triacetate-lactonase
* : actinomycin lactonase
* : orsellinate-depside hydrolase
* : cephalosporin-C deacetylase
* : chlorogenate hydrolase
* : α-amino-acid esterase
* : 4-methyloxaloacetate esterase
* : carboxymethylenebutenolidase
* : deoxylimonate A-ring-lactonase
* : 1-alkyl-2-acetylglycerophosphocholine esterase
* : fusarinine-C ornithinesterase
* : sinapine esterase
* : wax-ester hydrolase
* : phorbol-diester hydrolase
* : phosphatidylinositol deacylase
* : sialate ''O''-acetylesterase
* : acetoxybutynylbithiophene deacetylase
* : acetylsalicylate deacetylase
* : methylumbelliferyl-acetate deacetylase
* : 2-pyrone-4,6-dicarboxylate lactonase
* : ''N''-acetylgalactosaminoglycan deacetylase
* : juvenile-hormone esterase
* : bis(2-ethylhexyl)phthalate esterase
* : protein-glutamate methylesterase
* EC 3.1.1.62: Now listed as , ''N''-acetyldiaminopimelate deacetylase
* : 11-''cis''-retinyl-palmitate hydrolase
* : retinoid isomerohydrolase
* : L-rhamnono-1,4-lactonase
* : 5-(3,4-diacetoxybut-1-ynyl)-2,2′-bithiophene deacetylase
* : fatty-acyl-ethyl-ester synthase
* : xylono-1,4-lactonase
* EC 3.1.1.69: Now , ''N''-acetylglucosaminylphosphatidylinositol deacetylase
* : cetraxate benzylesterase
* : acetylalkylglycerol acetylhydrolase
* : acetylxylan esterase
* : feruloyl esterase
* : cutinase
* : poly(3-hydroxybutyrate) depolymerase
* : poly(3-hydroxyoctanoate) depolymerase
* : acyloxyacyl hydrolase
* : polyneuridine-aldehyde esterase
* : hormone-sensitive lipase
* : acetylajmaline esterase
* : quorum-quenching ''N''-acyl-homoserine lactonase
* : pheophorbidase
* : monoterpene ε-lactone hydrolase
* : cocaine esterase
* : pimelyl-(acyl-carrier protein) methyl ester esterase
* : rhamnogalacturonan acetylesterase
* : fumonisin B1 esterase
* : pyrethroid hydrolase
* : protein phosphatase methylesterase-1
* : ''all-trans''-retinyl ester 13-''cis'' isomerohydrolase
* : 2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase
* : 4-sulfomuconolactone hydrolase
* : mycophenolic acid acyl-glucuronide esterase
* : versiconal hemiacetal acetate esterase *
* : aclacinomycin methylesterase *
* : D-aminoacyl-tRNA deacylase *
* : methylated diphthine methylhydrolase *
* : nt protein''O''-palmitoleoyl-L-serine hydrolase *
* : 6-deoxy-6-sulfogluconolactonase *
* : chlorophyllide a hydrolase *
* : poly(ethylene terephthalate) hydrolase *
* : mono(ethylene terephthalate) hydrolase *
* : teichoic acid D-alanine hydrolase *
* : 5-phospho-D-xylono-1,4-lactonase *
* : 3-''O''-acetylpapaveroxine carboxylesterase *
* : ''O''-acetyl-ADP-ribose deacetylase *
* : apo-salmochelin esterase *
* : iron(III)-enterobactin esterase *
* : iron(III)-salmochelin esterase *
* : xylono-1,5-lactonase *
* : phosphatidylserine ''sn''-1 acylhydrolase *
* : isoamyl acetate esterase *
* : ethyl acetate hydrolase *
* : methyl acetate hydrolase
* : D-apionolactonase *
* : ''sn''-1-specific diacylglycerol lipase *
* : (4-''O''-methyl)-D-glucuronate—lignin esterase *
* : phospholipid ''sn''-1 acylhydrolase *
: *No Wikipedia article

EC 3.1.2: Thioester Hydrolases

* : acetyl-CoA hydrolase
* : palmitoyl-CoA hydrolase
* : succinyl-CoA hydrolase
* : 3-hydroxyisobutyryl-CoA hydrolase
* : hydroxymethylglutaryl-CoA hydrolase
* : hydroxyacylglutathione hydrolase
* : glutathione thiolesterase
* EC 3.1.2.8: Now included with hydroxyacylglutathione hydrolase
* EC 3.1.2.9: ''S''-acetoacetylhydrolipoate hydrolase deleted
* : formyl-CoA hydrolase
* : acetoacetyl-CoA hydrolase
* : ''S''-formylglutathione hydrolase
* : ''S''-succinylglutathione hydrolase
* : oleoyl- cyl-carrier-proteinhydrolase
* EC 3.1.2.15: covered by , ubiquitinyl hydrolase 1
* : citrate lyase deacetylase
* : (''S'')-methylmalonyl-CoA hydrolase
* : ADP-dependent short-chain-acyl-CoA hydrolase
* : ADP-dependent medium-chain-acyl-CoA hydrolase
* : acyl-CoA hydrolase
* : dodecanoyl-(acyl-carrier-protein) hydrolase
* : palmitoyl roteinhydrolase
* : 4-hydroxybenzoyl-CoA thioesterase
* EC 3.1.2.24: transferred entry now , 2′-hydroxybiphenyl-2-sulfinate desulfinase.
* : phenylacetyl-CoA hydrolase
* EC 3.1.2.26: Now , bile acid CoA transferase
* : choloyl-CoA hydrolase
* : 1,4-dihydroxy-2-naphthoyl-CoA hydrolase
* : fluoroacetyl-CoA thioesterase
* : (3''S'')-malyl-CoA thioesterase *
* : dihydromonacolin L- ovastatin nonaketide synthasethioesterase *
* : 2-aminobenzoylacetyl-CoA thioesterase *
: *No Wikipedia article

EC 3.1.3: Phosphoric Monoester Hydrolases

* : alkaline phosphatase
* : acid phosphatase
* : phosphoserine phosphatase
* : phosphatidate phosphatase
* : 5′-nucleotidase
* : 3′-nucleotidase
* : 3′(2′),5′-bisphosphate nucleotidase
* : 3-phytase
* : glucose-6-phosphatase
* : glucose-1-phosphatase
* : fructose-bisphosphatase
* : trehalose-phosphatase
* EC 3.1.3.13: Recent studies have shown that this is a partial activity of , phosphoglycerate mutase (2,3-diphosphoglycerate-dependent)
* : methylphosphothioglycerate phosphatase
* : histidinol-phosphatase
* : protein serine/threonine phosphatase
* : (phosphorylase) phosphatase
* : phosphoglycolate phosphatase
* : glycerol-2-phosphatase
* : phosphoglycerate phosphatase
* : glycerol-1-phosphatase
* : mannitol-1-phosphatase
* : sugar-phosphatase
* : sucrose-phosphatase
* : inositol-phosphate phosphatase
* : 4-phytase
* : phosphatidylglycerophosphatase
* : ADP-phosphoglycerate phosphatase
* : ''N''-acylneuraminate-9-phosphatase
* EC 3.1.3.30: The activity may be that of an acid phosphatase
* EC 3.1.3.31: The activity may be that of an acid phosphatase
* : polynucleotide 3′-phosphatase
* : polynucleotide 5′-phosphatase
* : deoxynucleotide 3′-phosphatase
* : thymidylate 5′-phosphatase
* : phosphoinositide 5-phosphatase
* : sedoheptulose-bisphosphatase
* : 3-phosphoglycerate phosphatase
* : streptomycin-6-phosphatase
* : guanidinodeoxy-''scyllo''-inositol-4-phosphatase
* : 4-nitrophenylphosphatase
* : glycogen-synthase-Dphosphatase
* : (pyruvate dehydrogenase (acetyl-transferring))-phosphatase"> cetyl-CoA carboxylasephosphatase
* : 3-deoxy-''manno''-octulosonate-8-phosphatase
* : fructose-2,6-bisphosphate 2-phosphatase
* : ydroxymethylglutaryl-CoA reductase (NADPH)phosphatase
* : protein-tyrosine-phosphatase
* : yruvate kinasephosphatase
* : sorbitol-6-phosphatase
* : dolichyl-phosphatase
* : -methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)phosphatase
* : yosin-light-chainphosphatase
* : fructose-2,6-bisphosphate 6-phosphatase
* : caldesmon-phosphatase
* : inositol-polyphosphate 5-phosphatase
* : inositol-1,4-bisphosphate 1-phosphatase
* : sugar-terminal-phosphatase
* : alkylacetylglycerophosphatase
* : phospho''enol''pyruvate phosphatase
* EC 3.1.3.61: deleted, as its existence has not been established
* : multiple inositol-polyphosphate phosphatase
* : 2-carboxy-D-arabinitol-1-phosphatase
* : phosphatidylinositol-3-phosphatase
* EC 3.1.3.65: Now included with , phosphatidylinositol-3-phosphatase
* : phosphatidylinositol-3,4-bisphosphate 4-phosphatase
* : phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase
* : 2-deoxyglucose-6-phosphatase
* : glucosylglycerol 3-phosphatase
* : mannosyl-3-phosphoglycerate phosphatase
* : 2-phosphosulfolactate phosphatase
* : 5-phytase
* : adenosylcobalamin/α-ribazole phosphatase
* : pyridoxal phosphatase
* : phosphoethanolamine/phosphocholine phosphatase
* : lipid-phosphate phosphatase
* : acireductone synthase
* : phosphatidylinositol-4,5-bisphosphate 4-phosphatase
* : mannosylfructose-phosphate phosphatase
* : 2,3-bisphosphoglycerate 3-phosphatase
* EC 3.1.3.81: Transferred entry, now , diacylglycerol diphosphate phosphatase
* : D-''glycero''-β-D-''manno''-heptose 1,7-bisphosphate 7-phosphatase
* : D-''glycero''-α-D-''manno''-heptose 1,7-bisphosphate 7-phosphatase
* : ADP-ribose 1′′-phosphate phosphatase
* : glucosyl-3-phosphoglycerate phosphatase
* : phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase
* : 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
* : 5′′-phosphoribostamycin phosphatase
: * No Wikipedia article

EC 3.1.4: Phosphoric Diester Hydrolases

* : phosphodiesterase I
* : glycerophosphocholine phosphodiesterase
* : lecithinase C
* : phospholipase D
* EC 3.1.4.5: Now , deoxyribonuclease I
* EC 3.1.4.6: Now , deoxyribonuclease II
* EC 3.1.4.7: Now , micrococcal nuclease
* EC 3.1.4.8: Now , ribonuclease T1
* EC 3.1.4.9: Now , ''Serratia marcescens'' nuclease
* EC 3.1.4.10: Now , phosphatidylinositol diacylglycerol-lyase
* : phosphoinositide phospholipase C
* : sphingomyelin phosphodiesterase
* : serine-ethanolaminephosphate phosphodiesterase
* : cyl-carrier-proteinphosphodiesterase
* : transferred to EC 2.7.7.89, adenylyl- lutamateammonia ligasephosphorylase
* : 2′,3′-cyclic-nucleotide 2′-phosphodiesterase
* : 3′,5′-cyclic-nucleotide phosphodiesterase
* EC 3.1.4.18: Now , spleen exonuclease
* EC 3.1.4.19: Now , oligonucleotidase
* EC 3.1.4.20: Now , exoribonuclease II
* EC 3.1.4.21: Now , ''Aspergillus'' nuclease S₁
* EC 3.1.4.22: Now , pancreatic ribonuclease
* EC 3.1.4.23: Now , ribonuclease T₂
* EC 3.1.4.24: deleted
* EC 3.1.4.25: Now , exodeoxyribonuclease I
* EC 3.1.4.26: deleted
* EC 3.1.4.27: Now , exodeoxyribonuclease III
* EC 3.1.4.28: Now , exodeoxyribonuclease (lambda-induced)
* EC 3.1.4.29: deleted
* EC 3.1.4.30: Now , deoxyribonuclease IV (phage-T₄-induced)
* EC 3.1.4.31: Now
* EC 3.1.4.32: deleted
* EC 3.1.4.33: deleted
* EC 3.1.4.34: deleted
* : 3′,5′-cyclic-GMP phosphodiesterase
* EC 3.1.4.36: Now with
* : 2′,3′-cyclic-nucleotide 3'-phosphodiesterase
* : glycerophosphocholine cholinephosphodiesterase
* : alkylglycerophosphoethanolamine phosphodiesterase
* : CMP-''N''-acylneuraminate phosphodiesterase
* : sphingomyelin phosphodiesterase D
* : glycerol-1,2-cyclic-phosphate 2-phosphodiesterase
* : glycerophosphoinositol inositolphosphodiesterase
* : glycerophosphoinositol glycerophosphodiesterase
* : ''N''-acetylglucosamine-1-phosphodiester α-''N''-acetylglucosaminidase
* : glycerophosphodiester phosphodiesterase
* EC 3.1.4.47: Now , glycosylphosphatidylinositol diacylglycerol-lyase
* : dolichylphosphate-glucose phosphodiesterase
* : dolichylphosphate-mannose phosphodiesterase
* : glycosylphosphatidylinositol phospholipase D
* : glucose-1-phospho-D-mannosylglycoprotein phosphodiesterase
* : cyclic-guanylate-specific phosphodiesterase
* : 3′,5′-cyclic-AMP phosphodiesterase
* : ''N''-acetylphosphatidylethanolamine-hydrolysing phospholipase D
* : phosphoribosyl 1,2-cyclic phosphate phosphodiesterase *
* : 7,8-dihydroneopterin 2′,3′-cyclic phosphate phosphodiesterase
* : phosphoribosyl 1,2-cyclic phosphate 1,2-diphosphodiesterase
* : RNA 2′,3′-cyclic 3′-phosphodiesterase *
* : cyclic-di-AMP phosphodiesterase *
* : pApA phosphodiesterase *
* : cyclic 2,3-diphosphoglycerate hydrolase *
: *No Wikipedia article

EC 3.1.5: Triphosphoric Monoester Hydrolases

* : dGTPase

EC 3.1.6: Sulfuric Ester Hydrolases

* : arylsulfatase (type I)
* : steryl-sulfatase
* : glycosulfatase
* : ''N''-acetylgalactosamine-6-sulfatase
* EC 3.1.6.5: deleted
* : choline-sulfatase
* : cellulose-polysulfatase
* : cerebroside-sulfatase
* : chondro-4-sulfatase
* : chondro-6-sulfatase
* : disulfoglucosamine-6-sulfatase
* : ''N''-acetylgalactosamine-6-sulfatase
* : iduronate-2-sulfatase
* : ''N''-acetylglucosamine-6-sulfatase
* : ''N''-sulfoglucosamine-3-sulfatase
* : monomethyl-sulfatase
* : D-lactate-2-sulfatase
* : Glucuronate-2-sulfatase
* : (''R'')-specific secondary-alkylsulfatase (type III) *
* : ''S''-sulfosulfanyl-L-cysteine sulfohydrolase *
* : linear primary-alkylsulfatase *
* : branched primary-alkylsulfatase *
: *No Wikipedia article

EC 3.1.7: Diphosphoric Monoester Hydrolases

* : prenyl-diphosphatase
* : guanosine-3′,5′-bis(diphosphate) 3′-diphosphatase
* : monoterpenyl-diphosphatase
* EC 3.1.7.4: Now recognized as two enzymes , copal-8-ol diphosphate synthase and , sclareol synthase
* : geranylgeranyl diphosphate diphosphatase
* : farnesyl diphosphatase
* EC 3.1.7.7: Now , (–)-drimenol synthase
* EC 3.1.7.8: Now known to be a partial activity of , adenosine tuberculosinyltransferase.
* EC 3.1.7.9: Now known to be a partial activity of , adenosine tuberculosinyltransferase
* : (13''E'')-labda-7,13-dien-15-ol synthase
* : geranyl diphosphate diphosphatase
* : (+)-kolavelool synthase *
: *No Wikipedia article

EC 3.1.8: Phosphoric Triester Hydrolases

* : aryldialkylphosphatase
* : diisopropyl-fluorophosphatase

EC 3.1.11: Exodeoxyribonucleases Producing 5'-Phosphomonoesters

* : exodeoxyribonuclease I
* : exodeoxyribonuclease III
* : exodeoxyribonuclease (lambda-induced)
* : exodeoxyribonuclease (phage SP₃-induced)
* : exodeoxyribonuclease V
* : exodeoxyribonuclease VII
* EC 3.1.11.7: Now , adenosine-5′-diphospho-5′- NAdiphosphatase
* EC 3.1.11.8: Now , guanosine-5′-diphospho-5′- NAdiphosphatase

EC 3.1.13: Exoribonucleases Producing 5'-Phosphomonoesters

* : exoribonuclease II
* : exoribonuclease H
* : oligonucleotidase
* : poly(A)-specific ribonuclease
* : ribonuclease D

EC 3.1.14: Exoribonucleases Producing 3'-Phosphomonoesters

* : yeast ribonuclease

EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters

* : venom exonuclease

EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters

* : spleen exonuclease

EC 3.1.21: Endodeoxyribonucleases Producing 5'-Phosphomonoesters

* : deoxyribonuclease I
* : deoxyribonuclease IV
* : type I site-specific deoxyribonuclease
* : type II site-specific deoxyribonuclease
* : type III site-specific deoxyribonuclease
* : CC-preferring endodeoxyribonuclease
* : deoxyribonuclease V
* : T₄ deoxyribonuclease II *
* : T₄ deoxyribonuclease IV *
* : crossover junction endodeoxyribonuclease *
: *No Wikipedia article

EC 3.1.22: Endodeoxyribonucleases Producing 3'-Phosphomonoesters

* : deoxyribonuclease II
* : Aspergillus deoxyribonuclease K1
* EC 3.1.22.3: now
* : crossover junction endodeoxyribonuclease
* : deoxyribonuclease X

EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5

Deleted sub-subclasses.

EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases

* : deoxyribonuclease (pyrimidine dimer)
* EC 3.1.25.2: Now , DNA-(apurinic or apyrimidinic site) lyase

EC 3.1.26: Endoribonucleases Producing 5'-Phosphomonoesters

* : ''Physarum polycephalum'' ribonuclease
* : ribonuclease α
* : ribonuclease III
* : ribonuclease H
* : ribonuclease P
* : ribonuclease IV
* : ribonuclease P4
* : ribonuclease M5
* : ribonuclease (poly-(U)-specific)
* : ribonuclease IX
* : tRNase Z
* : ribonuclease E
* : retroviral ribonuclease H

EC 3.1.27: Endoribonucleases Producing 3'-Phosphomonoesters

* EC 3.1.27.1: Now , ribonuclease T₂, since the primary reaction is that of a lyase
* EC 3.1.27.2: Now , ''Bacillus subtilis'' ribonuclease, since the reaction catalysed is that of a lyase
* EC 3.1.27.3: Now , ribonuclease T₁, since the primary reaction is that of a lyase
* EC 3.1.27.4: Now , ribonuclease U₂, since the primary reaction is that of a lyase
* EC 3.1.27.5: Now , pancreatic ribonuclease.
* EC 3.1.27.6: Now , ''Enterobacter'' ribonuclease, since the primary reaction is that of a lyase
* : ribonuclease F
* : ribonuclease V
* EC 3.1.27.9: Now , tRNA-intron lyase
* EC 3.1.27.10: Now , ribotoxin,

EC 3.1.30: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters

* : ''Aspergillus'' nuclease S₁
* : ''Serratia marcescens'' nuclease

EC 3.1.31: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters

* : micrococcal nuclease

EC 3.2: Glycosylases

EC 3.2.1: Glycosidases, i.e. enzymes hydrolysing ''O''- and ''S''-glycosyl compounds

* : α-amylase
* : β-amylase
* : glucan 1,4-α-glucosidase
* : cellulase
* : deleted
* : endo-1,3(4)-β-glucanase
* : inulinase
* : endo-1,4-β-xylanase
* : deleted
* : oligo-1,6-glucosidase
* : dextranase
* : Now included with , cyclomaltodextrinase
* : Now included with , cyclomaltodextrinase
* : chitinase
* : polygalacturonase
* : deleted
* : lysozyme
* : exo-α-sialidase
* : deleted
* : α-glucosidase
* : β-glucosidase
* : α-galactosidase
* : β-galactosidase
* : α-mannosidase
* : β-mannosidase
* : β-fructofuranosidase (invertase)
* : deleted
* : α,α-trehalase
* : Now included with , β-''N''-acetylhexosaminidase
* : Now included with , β-''N''-acetylhexosaminidase
* : β-glucuronidase
* : endo-1,3-β-xylanase
* : amylo-α-1,6-glucosidase
* : Now included with , hyaluronoglucosaminidase
* : hyaluronoglucosaminidase
* : hyaluronoglucuronidase
* : xylan 1,4-β-xylosidase
* : β-D-fucosidase
* : glucan endo-1,3-β-D-glucosidase
* : α-L-rhamnosidase
* : pullulanase
* : GDP-glucosidase
* : β-L-rhamnosidase
* : Now , endo-(13)-fucoidanase and , endo-(14)-fucoidanase
* : glucosylceramidase
* : galactosylceramidase
* : Now known to be catalyzed by , α-galactosidase
* : sucrose α-glucosidase
* : α-''N''-acetylgalactosaminidase
* : α-''N''-acetylglucosaminidase
* : α-L-fucosidase
* : β-''N''-acetylhexosaminidase
* : β-''N''-acetylgalactosaminidase
* : cyclomaltodextrinase
* : non-reducing end α-L-arabinofuranosidase
* : glucuronosyl-disulfoglucosamine glucuronidase
* : isopullulanase
* : glucan 1,3-β-glucosidase
* : glucan endo-1,3-α-glucosidase
* : glucan 1,4-α-maltotetraohydrolase
* : mycodextranase
* : glycosylceramidase
* : 1,2-α-L-fucosidase
* : 2,6-β-fructan 6-levanbiohydrolase
* : levanase
* : Deleted entry: The activity is covered by , α-L-rhamnosidase
* : galacturan 1,4-α-galacturonidase
* : isoamylase
* : Now included with , pullulanase
* : glucan 1,6-α-glucosidase
* : glucan endo-1,2-β-glucosidase
* : xylan 1,3-β-xylosidase
* : licheninase
* : glucan 1,4-β-glucosidase
* : glucan endo-1,6-β-glucosidase
* : L-iduronidase
* : mannan 1,2-(1,3)-α-mannosidase
* : mannan endo-1,4-β-mannosidase
* : Now included with , non-reducing end α-L-arabinofuranosidase
* : fructan β-fructosidase
* : β-agarase
* : exo-poly-α-digalacturonosidas
* : κ-carrageenase
* : glucan 1,3-α-glucosidase
* : 6-phospho-β-galactosidase
* : 6-phospho-β-glucosidase
* : capsular-polysaccharide endo-1,3-α-galactosidase
* : non-reducing end β-L-arabinopyranosidase
* : arabinogalactan endo-β-1,4-galactanase
* : Deleted, not sufficiently characterised.
* : cellulose 1,4-β-cellobiosidase (non-reducing end)
* : peptidoglycan β-''N''-acetylmuramidase
* : α,α-phosphotrehalase
* : glucan 1,6-α-isomaltosidase
* : dextran 1,6-α-isomaltotriosidase
* : mannosyl-glycoprotein endo-β-''N''-acetylglucosaminidase
* : endo-α-''N''-acetylgalactosaminidase
* : glucan 1,4-α-maltohexaosidase
* : arabinan endo-1,5-α-L-arabinanase
* : mannan 1,4-mannobiosidase
* : mannan endo-1,6-α-mannosidase
* : blood-group-substance endo-1,4-β-galactosidase
* : keratan-sulfate endo-1,4-β-galactosidase
* : steryl-β-glucosidase
* : 3α(''S'')-strictosidine β-glucosidase
* : mannosyl-oligosaccharide glucosidase
* : protein-glucosylgalactosylhydroxylysine glucosidase
* : lactase
* : endogalactosaminidase
* : identical to , endo-α-''N''-acetylgalactosaminidase
* : 1,3-α-L-fucosidase
* : 2-deoxyglucosidase
* : mannosyl-oligosaccharide 1,2-α-mannosidase
* : mannosyl-oligosaccharide 1,3-1,6-α-mannosidas
* : branched-dextran exo-1,2-α-glucosidase
* : glucan 1,4-α-maltotriohydrolase
* : amygdalin β-glucosidase
* : prunasin β-glucosidase
* : vicianin β-glucosidase
* : oligoxyloglucan β-glycosidase
* : polymannuronate hydrolase
* : maltose-6′-phosphate glucosidase
* : endoglycosylceramidase
* : 3-deoxy-2-octulosonidase
* : raucaffricine β-glucosidase
* : coniferin β-glucosidase
* : 1,6-α-L-fucosidase
* : glycyrrhizin hydrolase
* : endo-α-sialidase
* : glycoprotein endo-α-1,2-mannosidase
* : xylan α-1,2-glucuronosidase
* : chitosanase
* : glucan 1,4-α-maltohydrolase
* : difructose-anhydride synthase
* : neopullulanase
* : glucuronoarabinoxylan endo-1,4-β-xylanase
* : mannan exo-1,2-1,6-α-mannosidase
* EC 3.2.1.138: Now , anhydrosialidase
* : α-glucuronidase
* : lacto-''N''-biosidase
* : 4-α-D-trehalose trehalohydrolase
* : limit dextrinase
* : poly(ADP-ribose) glycohydrolase
* : 3-deoxyoctulosonase
* : galactan 1,3-β-galactosidase
* : β-galactofuranosidase
* : thioglucosidase
* EC 3.2.1.148: The activity is most probably attributable to , ''S''-ribosylhomocysteine lyase
* : β-primeverosidase
* : oligoxyloglucan reducing-end-specific cellobiohydrolase
* : xyloglucan-specific endo-β-1,4-glucanase
* : mannosylglycoprotein endo-β-mannosidase
* : fructan β-(2,1)-fructosidase
* : fructan β-(2,6)-fructosidase
* : xyloglucan-specific endo-processive β-1,4-glucanase
* : oligosaccharide reducing-end xylanase
* : ι-carrageenase
* : α-agarase
* : α-neoagaro-oligosaccharide hydrolase
* EC 3.2.1.160: identical to , xyloglucan-specific exo-β-1,4-glucanase
* : β-apiosyl-β-glucosidase
* : λ-carrageenase
* : 1,6-α-D-mannosidase
* : galactan endo-1,6-β-galactosidase
* : exo-1,4-β-D-glucosaminidase
* : heparanase
* : baicalin-β-D-glucuronidase
* : hesperidin 6-''O''-α-L-rhamnosyl-β-D-glucosidase
* : protein ''O''-GlcNAcas
* : mannosylglycerate hydrolase
* : rhamnogalacturonan hydrolase
* : unsaturated rhamnogalacturonyl hydrolase
* : rhamnogalacturonan galacturonohydrolase
* : rhamnogalacturonan rhamnohydrolase
* : β-D-glucopyranosyl abscisate β-glucosidase
* : cellulose 1,4-β-cellobiosidase (reducing end)
* : α-D-xyloside xylohydrolase
* : β-porphyranase
* : gellan tetrasaccharide unsaturated glucuronyl hydrolase
* : unsaturated chondroitin disaccharide hydrolase
* : galactan endo-β-1,3-galactanase
* : 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2''H''-1,4-benzoxazin-2-yl glucoside β-D-glucosidase
* : UDP-''N''-acetylglucosamine 2-epimerase (hydrolysing)
* : UDP-''N,N''′-diacetylbacillosamine 2-epimerase (hydrolysing)
* : non-reducing end β-L-arabinofuranosidase *
* : protodioscin 26-''O''-β-D-glucosidase *
* : (Ara-f)3-Hyp β-L-arabinobiosidase *
* : avenacosidase *
* : dioscin glycosidase (diosgenin-forming) *
* : dioscin glycosidase (3-''O''-β-D-Glc-diosgenin-forming) *
* : ginsenosidase type III *
* : ginsenoside Rb1 β-glucosidase *
* : ginsenosidase type I *
* : ginsenosidase type IV *
* : 20-''O''-multi-glycoside ginsenosidase *
* : limit dextrin α-1,6-maltotetraose-hydrolase *
* : β-1,2-mannosidase *
* : α-mannan endo-1,2-α-mannanase *
* : sulfoquinovosidase *
* : exo-chitinase (non-reducing end) *
* : exo-chitinase (reducing end) *
* : endo-chitodextinase *
* : carboxymethylcellulase *
* : 1,3-α-isomaltosidase *
* : isomaltose glucohydrolase *
* : oleuropein β-glucosidase *
* : mannosyl-oligosaccharide α-1,3-glucosidase *
* : glucosylglycerate hydrolase *
* : endoplasmic reticulum Man9GlcNAc2 1,2-α-mannosidase *
* : endoplasmic reticulum Man8GlcNAc2 1,2-α-mannosidase *
* : endo-(1→3)-fucoidanase *
* : endo-(1→4)-fucoidanase *
* : galactan exo-1,6-β-galactobiohydrolase (non-reducing end) *
* : exo β-1,2-glucooligosaccharide sophorohydrolase (non-reducing end) *
: *No Wikipedia article

EC 3.2.2: Hydrolysing ''N''-Glycosyl Compounds

* : purine nucleosidase
* : inosine nucleosidase
* : uridine nucleosidase
* : AMP nucleosidase
* : NAD⁺ glycohydrolase
* : ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
* : adenosine nucleosidase
* : ribosylpyrimidine nucleosidase
* : adenosylhomocysteine nucleosidase
* : pyrimidine-5′-nucleotide nucleosidase
* : β-aspartyl-''N''-acetylglucosaminidase
* : inosinate nucleosidase
* : 1-methyladenosine nucleosidase
* : NMN nucleosidase
* : DNA-deoxyinosine glycosylase
* : methylthioadenosine nucleosidase
* : deoxyribodipyrimidine endonucleosidase
* EC 3.2.2.18: Now included with , peptide-N⁴-(''N''-acetyl-β-glucosaminyl)asparagine amidase
* : ADP-ribosylarginine hydrolase
* : DNA-3-methyladenine glycosylase I
* : DNA-3-methyladenine glycosylase II, MAG1
* : rRNA N''-glycosylase
* : DNA-formamidopyrimidine glycosylase
* : ADP-ribosyl- initrogen reductasehydrolase
* : ''N''-methyl nucleosidase
* : futalosine hydrolase
* : uracil-DNA glycosylase
* : double-stranded uracil-DNA glycosylase
* : thymine-DNA glycosylase
* : aminodeoxyfutalosine nucleosidase *
* : adenine glycosylase *
: *No Wikipedia article

EC 3.2.3: Hydrolysing ''S''-Glycosyl Compounds

Deleted sub-subclass

EC 3.3: Acting on Ether Bonds

EC 3.3.1: Thioether and trialkylsulfonium hydrolases

* : adenosylhomocysteinase
* : ''S''-adenosyl-L-methionine hydrolase (L-homoserine-forming)
* EC 3.3.1.3: The activity is most probably attributable to , ''S''-ribosylhomocysteine lyase

EC 3.3.2: Ether Hydrolases

* : isochorismatase
* : alkenylglycerophosphocholine hydrolase
* EC 3.3.2.3: Now known to comprise two enzymes, microsomal epoxide hydrolase () and soluble epoxide hydrolase ()
* : ''trans''-epoxysuccinate hydrolase
* EC 3.3.2.5: Now included in , lysoplasmalogenase
* : leukotriene-A₄ hydrolase
* : hepoxilin-epoxide hydrolase
* : limonene-1,2-epoxide hydrolase
* : microsomal epoxide hydrolase
* : soluble epoxide hydrolase
* : cholesterol-5,6-oxide hydrolase
* : oxepin-CoA hydrolase *
* : chorismatase *
* : 2,4-dinitroanisole ''O''-demethylase *
* : ''trans''-2,3-dihydro-3-hydroxyanthranilic acid synthase *
: *No Wikipedia article

EC 3.4: Acting on

peptide bonds – Peptidase

EC 3.4.1 α-amino acyl peptide hydrolases (discontinued)

* EC 3.4.1.1: Now , leucyl aminopeptidase
* EC 3.4.1.2: Now , membrane alanyl aminopeptidase
* EC 3.4.1.3: Now , tripeptide aminopeptidase
* EC 3.4.1.4: Now , prolyl aminopeptidase

EC 3.4.2 Peptidyl amino acid hydrolases (discontinued)

* EC 3.4.2.1: Now , carboxypeptidase A
* EC 3.4.2.2: Now , carboxypeptidase B
* EC 3.4.2.3: Now , Gly-Xaa carboxypeptidase

EC 3.4.3: Dipeptide hydrolases (deleted sub-subclass)

* EC 3.4.3.1: Now , cytosol nonspecific dipeptidase
* EC 3.4.3.2: Now , cytosol nonspecific dipeptidase
* EC 3.4.3.3: Now , Xaa-His dipeptidase
* EC 3.4.3.4: Now , Xaa-methyl-His dipeptidase
* EC 3.4.3.5: Now , membrane alanyl aminopeptidase
* EC 3.4.3.6: Now , cytosol nonspecific dipeptidase
* EC 3.4.3.7: Now , Xaa-Pro dipeptidase

EC 3.4.4 Peptidyl Peptide Hydrolases (discontinued)

* EC 3.4.4.1: Now , pepsin A
* EC 3.4.4.2: Now , pepsin B
* EC 3.4.4.3: Now , chymosin
* EC 3.4.4.4: Now , trypsin
* EC 3.4.4.5: Now , chymotrypsin
* EC 3.4.4.6: Now , chymotrypsin
* EC 3.4.4.7: Now covered by , pancreatic elastase and , leukocyte elastase
* EC 3.4.4.8: Now , enteropeptidase
* EC 3.4.4.9: Now , dipeptidyl-peptidase I
* EC 3.4.4.10: Now , papain
* EC 3.4.4.11: Now , chymopapain
* EC 3.4.4.12: Now , ficain
* EC 3.4.4.13: Now , thrombin
* EC 3.4.4.14: Now , plasmin
* EC 3.4.4.15: Now , renin
* EC 3.4.4.16: Now covered by the microbial serine proteinases (subtilisin), (oryzin), (endopeptidase K), (thermomycolin), (thermitase) and (endopeptidase So)
* EC 3.4.4.17: Now covered by the microbial aspartic proteinases (penicillopepsin), (rhizopuspepsin), (endothiapepsin), (mucorpepsin), (candidapepsin), (saccharopepsin), (rhodotorulapepsin), (physarolisin), (acrocylindropepsin), (polyporopepsin) and (pycnoporopepsin)
* EC 3.4.4.18: Now , streptopain
* EC 3.4.4.19: Now , microbial collagenase
* EC 3.4.4.20: Now , clostripain
* EC 3.4.4.21: Now (plasma kallikrein) and (tissue kallikrein)
* EC 3.4.4.22: Now , gastricsin
* EC 3.4.4.23: Now , cathepsin D
* EC 3.4.4.24: Now covered by (stem bromelain) and (fruit bromelain)
* EC 3.4.4.25: deleted

EC 3.4.11

Aminopeptidases

* : leucyl aminopeptidase
* : membrane alanyl aminopeptidase
* : cystinyl aminopeptidase
* : tripeptide aminopeptidase
* : prolyl aminopeptidase
* : aminopeptidase B
* : glutamyl aminopeptidase
* EC 3.4.11.8: Now EC 3.4.19.3, pyroglutamyl-peptidase I
* : Xaa-Pro aminopeptidase
* : bacterial leucyl aminopeptidase
* EC 3.4.11.11: Deleted
* EC 3.4.11.12: Deleted
* : Clostridial aminopeptidase
* : cytosol alanyl aminopeptidase
* : aminopeptidase Y
* : Xaa-Trp aminopeptidase
* : tryptophanyl aminopeptidase
* : methionyl aminopeptidase
* : D-stereospecific aminopeptidase
* : aminopeptidase Ey
* : aspartyl aminopeptidase
* : aminopeptidase I
* : PepB aminopeptidase
* : aminopeptidase S
* : β-peptidyl aminopeptidase
* : intermediate cleaving peptidase 55

EC 3.4.12 Peptidylamino-acid hydrolases or acylamino-acid hydrolases (deleted sub-subclass)

* EC 3.4.12.1: Now (carboxypeptidase C) and (carboxypeptidase D)
* EC 3.4.12.2: Now , carboxypeptidase A
* EC 3.4.12.3: Now , carboxypeptidase B
* EC 3.4.12.4: Now , lysosomal Pro-Xaa carboxypeptidase
* EC 3.4.12.5: Now , ''N''-acetylmuramoyl-L-alanine amidase
* EC 3.4.12.6: Now , muramoyl-pentapeptidase carboxypeptidase
* EC 3.4.12.7: Now , lysine carboxypeptidase
* EC 3.4.12.8: Now , Gly-Xaa carboxypeptidase
* EC 3.4.12.9: aspartate carboxypeptidase
* EC 3.4.12.10: Now , γ-glutamyl hydrolase
* EC 3.4.12.11: Now , alanine carboxypeptidase
* EC 3.4.12.12: Now (carboxypeptidase C) and (carboxypeptidase D)
* EC 3.4.12.13: γ-glutamylglutamate carboxypeptidase

EC 3.4.13 Dipeptidases

* EC 3.4.13.1: Now , cytosol nonspecific dipeptidase
* EC 3.4.13.2: Now , cytosol nonspecific dipeptidase
* EC 3.4.13.3: The activity is covered by , cytosol nonspecific dipeptidase and , β-Ala-His dipeptidase
* : Xaa-Arg dipeptidase
* : Xaa-methyl-His dipeptidase
* EC 3.4.13.6: Now , membrane alanyl aminopeptidase
* : Glu-Glu dipeptidase
* EC 3.4.13.8: Now , cytosol nonspecific dipeptidase
* : Xaa-Pro dipeptidase
* EC 3.4.13.10: Now , β-aspartyl-peptidase
* EC 3.4.13.11: Now , membrane dipeptidase
* : Met-Xaa dipeptidase
* EC 3.4.13.13: Now , Xaa-His dipeptidase
* EC 3.4.13.14: Deleted
* EC 3.4.13.15: Now , cytosol nonspecific dipeptidase
* : Deleted
* : non-stereospecific dipeptidase
* : cytosol nonspecific dipeptidase
* : membrane dipeptidase
* : β-Ala-His dipeptidase
* : dipeptidase E
* : D-Ala-D-Ala dipeptidase
* : cysteinylglycine-''S''-conjugate dipeptidase *
: *No Wikipedia article

EC 3.4.14 Dipeptidyl peptidases and tripeptidyl peptidases

* : dipeptidyl-peptidase I
* : dipeptidyl-peptidase II
* EC 3.4.14.3: Now , acylaminoacyl-peptidase
* : dipeptidyl-peptidase III
* : dipeptidyl-peptidase IV
* : dipeptidyl-dipeptidase
* EC 3.4.14.7: Deleted
* EC 3.4.14.8: Now , tripeptidyl-peptidase II
* : tripeptidyl-peptidase I
* : tripeptidyl-peptidase II
* : Xaa-Pro dipeptidyl-peptidase
* : Xaa-Xaa-Pro tripeptidyl-peptidase
* : γ-D-glutamyl-Llysine dipeptidyl-peptidase
* : ycofactocin precursor peptidepeptidase *
: *No Wikipedia article

EC 3.4.15 Peptidyl dipeptidases

* : peptidyl-dipeptidase A
* EC 3.4.15.2: Now , peptidyl-glycinamidase
* EC 3.4.15.3: Now , peptidyl-dipeptidase Dcp
* : Peptidyl-dipeptidase B
* : Peptidyl-dipeptidase Dcp
* : cyanophycinase

EC 3.4.16 Serine type carboxypeptidases

* EC 3.4.16.1: Transferred entry: serine carboxypeptidase. Now , carboxypeptidase D
* : lysosomal Pro-Xaa carboxypeptidase
* EC 3.4.16.3: Now included with , carboxypeptidase C
* : serine-type D-Ala-D-Ala carboxypeptidase
* : carboxypeptidase C
* : carboxypeptidase D

EC 3.4.17 Metallocarboxypeptidases

* : carboxypeptidase A
* : carboxypeptidase B
* : lysine carboxypeptidase
* : Gly-Xaa carboxypeptidase
* EC 3.4.17.5: Deleted
* : alanine carboxypeptidase
* EC 3.4.17.7: Now , ''N''-acetylmuramoyl-L-alanine amidase
* : muramoylpentapeptide carboxypeptidase
* EC 3.4.17.9: Now included with , Gly-Xaa carboxypeptidase
* : carboxypeptidase E
* : glutamate carboxypeptidase
* : carboxypeptidase M
* : Muramoyltetrapeptide carboxypeptidase
* : zinc D-Ala-D-Ala carboxypeptidase
* : carboxypeptidase A₂
* : membrane Pro-Xaa carboxypeptidase
* : tubulinyl-Tyr carboxypeptidase
* : carboxypeptidase T
* : Carboxypeptidase Taq
* : carboxypeptidase U
* : Glutamate carboxypeptidase II
* : metallocarboxypeptidase D
* : angiotensin-converting enzyme 2
* : tubulin-glutamate carboxypeptidase *
: *No Wikipedia article

EC 3.4.18 Cysteine type carboxypeptidases

* : cathepsin X

EC 3.4.19 Omega peptidases

* : acylaminoacyl-peptidase
* : peptidyl-glycinamidase
* : pyroglutamyl-peptidase I
* EC 3.4.19.4: Deleted
* : β-aspartyl-peptidase
* : pyroglutamyl-peptidase II
* : ''N''-formylmethionyl-peptidase
* EC 3.4.19.8: now , glutamate carboxypeptidase II
* : folate γ-glutamyl hydrolase
* EC 3.4.19.10: Now , ''N''-acetylmuramoyl-L-alanine amidase
* : γ-Dglutamyl-''meso''-diaminopimelate peptidase
* : ubiquitinyl hydrolase 1
* : glutathione γ-glutamate hydrolase
* : leukotriene-C₄ hydrolase
* : desampylase *
* : glucosinolate γ-glutamyl hydrolase *
: *No Wikipedia article

EC 3.4.21:

Serine proteases

* : chymotrypsin
* : chymotrypsin C
* : metridin
* : trypsin
* : thrombin
* : coagulation factor Xa
* : plasmin
* EC 3.4.21.8: Now (plasma kallikrein) and (tissue kallikrein)
* : enteropeptidase
* : acrosin
* EC 3.4.21.11: Now , leukocyte elastase
* : α-lytic endopeptidase
* EC 3.4.21.13: Now , carboxypeptidase D
* EC 3.4.21.14: now , endopeptidase So
* EC 3.4.21.15: Now , oryzin
* EC 3.4.21.16: Deleted
* EC 3.4.21.17: Deleted
* EC 3.4.21.18: Deleted
* : glutamyl endopeptidase
* : cathepsin G
* : coagulation factor VIIa
* : coagulation factor IXa
* EC 3.4.21.23: Deleted
* EC 3.4.21.24: Deleted
* : cucumisin
* : prolyl oligopeptidase
* : coagulation factor XIa
* EC 3.4.21.28: Now , venombin A
* EC 3.4.21.29: Now , venombin A
* EC 3.4.21.30: Now , venombin A
* EC 3.4.21.31: Now , u-plasminogen activator
* : brachyurin
* EC 3.4.21.33: Deleted
* : plasma kallikrein
* : tissue kallikrein
* : pancreatic elastase
* : leukocyte elastase
* : coagulation factor XIIa
* : chymase
* EC 3.4.21.40: Deleted
* : Complement subcomponent C^1r
* : complement subcomponent C^1s
* : classical-complement-pathway C3/C5 convertase
* EC 3.4.21.44: Now , classical-complement-pathway C3/C5 convertase
* : complement factor I
* : complement factor D
* : alternative-complement-pathway C3/C5 convertase
* : cerevisin
* : hypodermin C
* : lysyl endopeptidase
* EC 3.4.21.51: Deleted
* EC 3.4.21.52: Deleted
* : edopeptidase La
* : γ-renin
* : venombin AB
* EC 3.4.21.56: Now considered to be , cucumisin
* : leucyl endopeptidase
* EC 3.4.21.58: Deleted
* : tryptase
* : scutelarin
* : kexin
* : subtilisin
* : oryzin
* : endopeptidase K
* : thermomycolin
* : thermitase
* : endopeptidase So
* : ''t''-plasminogen activator
* : protein C (activated)
* : pancreatic endopeptidase E
* : pancreatic elastase II
* : IgA-specific serine endopeptidase
* : u-plasminogen activator
* : venombin A
* : furin
* : myeloblastin
* : semenogelase
* : granzyme A
* : granzyme B
* : streptogrisin A
* : streptogrisin B
* : glutamyl endopeptidase II
* : oligopeptidase B
* : limulus clotting factor C
* : limulus clotting factor B
* : limulus clotting enzyme
* EC 3.4.21.87: Now , omptin
* : repressor LexA
* : signal peptidase I
* : togavirin
* : flavivirin
* : endopeptidase Clp
* : proprotein convertase 1
* : proprotein convertase 2
* : snake venom factor V activator
* : lactocepin
* : assemblin
* : hepacivirin
* : spermosin
* : sedolisin
* : xanthomonalisin
* : ''C''-terminal processing peptidase
* : physarolisin
* : mannan-binding lectin-associated serine protease-2
* : rhomboid protease
* : hepsin
* : peptidase Do
* : HtrA2 peptidase
* : matriptase
* : C5a peptidase
* : aqualysin 1
* : site-1 protease
* : pestivirus NS3 polyprotein peptidase
* : equine arterivirus serine peptidase
* : infectious pancreatic necrosis birnavirus Vp4 peptidase
* : SpoIVB peptidase
* : stratum corneum chymotryptic enzyme
* : kallikrein 8
* : kallikrein 13
* : oviductin
* : Lys-Lys/Arg-Xaa endopeptidase *
: *No Wikipedia article

EC 3.4.22

Cysteine proteases

* : cathepsin B
* : papain
* : ficain
* EC 3.4.22.4: Now (stem bromelain) and (fruit bromelain)
* EC 3.4.22.5: Now (stem bromelain) and (fruit bromelain)
* : chymopapain
* : asclepain
* : clostripain
* EC 3.4.22.9: Now , cerevisin
* : streptopain
* EC 3.4.22.11: Now , insulysin
* EC 3.4.22.12: Now , γ-glutamyl hydrolase
* EC 3.4.22.13: Deleted
* : actinidain
* : cathepsin L
* : cathepsin H
* EC 3.4.22.17: Now , calpain-2
* EC 3.4.22.18: Now , prolyl oligopeptidase
* EC 3.4.22.19: Now , thimet oligopeptidase
* EC 3.4.22.20: Deleted
* EC 3.4.22.21: Now , proteasome endopeptidase complex
* EC 3.4.22.22: Now , saccharolysin
* EC 3.4.22.23: Now , kexin
* : Cathepsin T
* : Glycyl endopeptidase
* : Cancer procoagulant
* : cathepsin S
* : picornain 3C
* : picornain 2A
* : Caricain
* : Ananain
* : Stem bromelain
* : Fruit bromelain
* : Legumain
* : Histolysain
* : caspase-1
* : Gingipain R
* : Cathepsin K
* : adenain
* : bleomycin hydrolase
* : cathepsin F
* : cathepsin O
* : cathepsin V
* : nuclear-inclusion-a endopeptidase
* : helper-component proteinase
* : L-peptidase, L-peptidase
* : gingipain K
* : staphopain
* : separase
* : V-cath endopeptidase
* : cruzipain
* : calpain-1
* : calpain-2
* : calpain-3
* : caspase-2
* : caspase-3
* : caspase-4
* : caspase-5
* : caspase-6
* : caspase-7
* : caspase-8
* : caspase-9
* : caspase-10
* : caspase-11
* : peptidase 1 (mite)
* : calicivirin
* : zingipain
* : Ulp1 peptidase
* : SARS coronavirus main proteinase
* : sortase A
* : sortase B

EC 3.4.23 Aspartic endopeptidases

* : pepsin A
* : pepsin B
* : gastricsin
* : chymosin
* : cathepsin D
* EC 3.4.23.6: Now , pycnoporopepsin
* EC 3.4.23.7: Now , penicillopepsin
* EC 3.4.23.8: Now , saccharopepsin
* EC 3.4.23.9: Now , rhizopuspepsin
* EC 3.4.23.10: Now , endothiapepsin
* EC 3.4.23.11: Deleted entry
* : nepenthesin
* EC 3.4.23.13: Deleted
* EC 3.4.23.14: Deleted
* : renin
* : HIV-1 retropepsin
* : Pro-opiomelanocortin converting enzyme, ''pro''-opiomelanocortin converting enzyme
* : aspergillopepsin I
* : aspergillopepsin II
* : penicillopepsin
* : rhizopuspepsin
* : endothiapepsin
* : mucorpepsin
* : candidapepsin
* : saccharopepsin
* : rhodotorulapepsin
* EC 3.4.23.27: Now , physarolisin
* : acrocylindropepsin
* : polyporopepsin
* : pycnoporopepsin
* : scytalidopepsin A
* : scytalidopepsin B
* EC 3.4.23.33: Now , xanthomonalisin
* : cathepsin E
* : barrierpepsin
* : signal peptidase II
* EC 3.4.23.37: Now , pseudomonalisin
* : plasmepsin I
* : plasmepsin II
* : phytepsin
* : yapsin 1
* : thermopsin
* : prepilin peptidase
* : nodavirus endopeptidase
* : memapsin 1
* : memapsin 2
* : HIV-2 retropepsin
* : plasminogen activator Pla
* : omptin
* : human endogenous retrovirus K endopeptidase
* : HycI peptidase
* : preflagellin peptidase

EC 3.4.24: Metallopeptidases

* : atrolysin A
* : Deleted entry: Sepia proteinase
* : microbial collagenase
* : now serralysin
* EC 3.4.24.5: Deleted entry: lens neutral proteinase. Now included with (calpain-2) and (proteasome endopeptidase complex)
* : leucolysin
* : interstitial collagenase
* EC 3.4.24.8: Transferred entry: ''Achromobacter iophagus'' collagenase. Now , microbial collagenase
* EC 3.4.24.9: Deleted entry: Trichophyton schoenleinii collagenase
* EC 3.4.24.10: Deleted entry: Trichophyton mentagrophytes keratinase
* : neprilysin
* : envelysin
* : IgA-specific metalloendopeptidase
* : procollagen N-endopeptidase
* : thimet oligopeptidase
* : neurolysin
* : stromelysin 1
* : meprin A
* : procollagen C-endopeptidase
* : peptidyl-Lys metalloendopeptidase
* : astacin
* : stromelysin 2
* : matrilysin
* : gelatinase a
* : vibriolysin
* : pseudolysin
* : thermolysin
* : bacillolysin
* : aureolysin
* : coccolysin
* : mycolysin
* : beta-Lytic metalloendopeptidase, β-lytic metalloendopeptidase
* : peptidyl-Asp metalloendopeptidase
* : neutrophil collagenase
* : gelatinase B
* : leishmanolysin
* : saccharolysin
* : gametolysin
* : deuterolysin
* : serralysin
* : atrolysin B
* : atrolysin C
* : atroxase
* : atrolysin E
* : atrolysin F
* : adamalysin
* : horrilysin
* : ruberlysin
* : bothropasin
* : bothrolysin
* : ophiolysin
* : trimerelysin I
* : trimerelysin II
* : mucrolysin
* : pitrilysin
* : insulysin
* : O-sialoglycoprotein endopeptidase, ''O''-sialoglycoprotein endopeptidase
* : russellysin
* : mitochondrial intermediate peptidase
* : dactylysin
* : nardilysin
* : magnolysin
* : meprin B
* : mitochondrial processing peptidase
* : macrophage elastase
* : choriolysin L
* : choriolysin H
* : tentoxilysin
* : bontoxilysin
* : oligopeptidase A
* : endothelin-converting enzyme 1
* : fibrolase
* : jararhagin
* : fragilysin
* : lysostaphin
* : flavastacin
* : snapalysin
* : gpr endopeptidase
* : pappalysin-1
* : membrane-type matrix metalloproteinase-1
* : ADAM10 endopeptidase
* : ADAMTS-4 endopeptidase
* : anthrax lethal factor endopeptidase
* : Ste24 endopeptidase
* : S2P endopeptidase
* : ADAM 17 endopeptidase
* : ADAMTS13 endopeptidase

EC 3.4.25 Threonine endopeptidases

* : proteasome endopeptidase complex
* : HslU—HslV peptidase

* EC 3.4.99.7: Deleted entry: euphorbain
* EC 3.4.99.8: Deleted entry: Gliocladium proteinase
* EC 3.4.99.9: Deleted entry: hurain. Now considered to be , cucumisin
* EC 3.4.99.10: Transferred entry: insulinase. Now , insulysin
* EC 3.4.99.11: Deleted entry: Streptomyces alkalophilic keratinase
* EC 3.4.99.12: Deleted entry: ''Trichophyton mentagrophytes'' keratinase
* EC 3.4.99.13: Transferred entry: β-lytic proteinase (''Mycobacterium sorangium''). Now , β-lytic metalloendopeptidase
* EC 3.4.99.14: Deleted entry: mexicanain
* EC 3.4.99.15: Deleted entry: Paecilomyces proteinase
* EC 3.4.99.16: Deleted entry: ''Penicillium notatum'' extracellular proteinase
* EC 3.4.99.17: Deleted entry: peptidoglycan endopeptidase
* EC 3.4.99.18: Deleted entry: pinguinain
* EC 3.4.99.19: Transferred entry: renin. Now , renin
* EC 3.4.99.20: Deleted entry: scopulariopsis proteinase
* EC 3.4.99.21: Deleted entry: solanain. Now considered , cucumisin
* EC 3.4.99.22: Transferred entry: staphylokinase. , aureolysin
* EC 3.4.99.23: Deleted entry: tabernamontanain. Now considered EC 3.4.21.25, cucumisin
* EC 3.4.99.24: Deleted entry: Tenebrio α-proteinase
* EC 3.4.99.25: Transferred entry: trametes acid proteinase. , rhizopuspepsin
* EC 3.4.99.26: Transferred entry: urokinase. Now , t-plasminogen activator
* EC 3.4.99.27: Deleted entry: ''Echis carinatus'' prothrombin-activating proteinase
* EC 3.4.99.28: Transferred entry: ''Oxyuranus scutellatus'' prothrombin-activating proteinase. , scutelarin
* EC 3.4.99.29: Deleted entry: Myxobacter AL-1 proteinase I
* EC 3.4.99.30: Transferred entry: Myxobacter AL-1 proteinase II. , peptidyl-Lys metalloendopeptidase
* EC 3.4.99.31: Transferred entry: tissue endopeptidase degrading collagenase synthetic substrate. , thimet oligopeptidase
* EC 3.4.99.32: Transferred entry: ''Armillaria mellea'' neutral proteinase. Now , peptidyl-Lys metalloendopeptidase
* EC 3.4.99.33: Deleted entry: cathepsin R
* EC 3.4.99.34: Deleted entry: mytilidase
* EC 3.4.99.35: Transferred entry: premurein-leader peptidase. Now , signal peptidase II
* EC 3.4.99.36: Transferred entry: leader peptidase. Now , signal peptidase I
* EC 3.4.99.37: Deleted entry: RecA peptidase
* EC 3.4.99.38: Transferred entry: pro-opiomelanotropin-converting proteinase. Now , pro-opiomelanocortin converting enzyme
* EC 3.4.99.39: Deleted entry: pseudomurein endopeptidase
* EC 3.4.99.40: Deleted entry: pro-gonadoliberin proteinase
* EC 3.4.99.41: Transferred entry: mitochondrial processing peptidase. Now , mitochondrial processing peptidase
* EC 3.4.99.42: Deleted entry: leucyllysine endopeptidase
* EC 3.4.99.43: Transferred entry: thermopsin. Npw , thermopsin
* EC 3.4.99.44: Transferred entry: pitrilysin. Now , pitrilysin
* EC 3.4.99.45: Transferred entry: insulinase. Now , insulysin
* EC 3.4.99.46: Transferred entry: multicatalytic endopeptidase complex. Now , proteasome endopeptidase complex

EC 3.5: Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1: In linear amides

* : asparaginase
* : glutaminase
* : omega-amidase, ω-amidase
* : amidase
* : urease
* : beta-ureidopropionase, β-ureidopropionase
* : ureidosuccinase
* : formylaspartate deformylase
* : arylformamidase
* : formyltetrahydrofolate deformylase
* : penicillin amidase
* : biotinidase
* : aryl-acylamidase
* : aminoacylase, ''N''-acyl-aliphatic-L-amino acid amidohydrolase
* : aspartoacylase
* : acetylornithine deacetylase
* : acyl-lysine deacylase
* : succinyl-diaminopimelate desuccinylase
* : nicotinamidase
* : citrullinase
* : N-acetyl-beta-alanine deacetylase, ''N''-acetyl-β-alanine deacetylas
* : pantothenase
* : ceramidase
* : choloylglycine hydrolase
* : N-acetylglucosamine-6-phosphate deacetylase, ''N''-acetylglucosamine-6-phosphate deacetylase
* : N4-(beta-N-acetylglucosaminyl)-L-asparaginase, ''N''⁴-(β-''N''-acetylglucosaminyl)-L-asparaginase
* EC 3.5.1.27: The activity is covered by , peptide deformylase
* : N-acetylmuramoyl-L-alanine amidase, ''N''-acetylmuramoyl-L-alanine amidase
* : 2-(acetamidomethylene)succinate hydrolase
* : 5-aminopentanamidase
* : formylmethionine deformylase
* : hippurate hydrolase
* : N-acetylglucosamine deacetylase, ''N''-acetylglucosamine deacetylase
* EC 3.5.1.34: Identical with , Xaa-methyl-His dipeptidase
* : D-glutaminase, D-glutaminase
* : N-methyl-2-oxoglutaramate hydrolase, ''N''-methyl-2-oxoglutaramate hydrolase
* EC 3.5.1.37: Delete, identical with ''N''⁴-(β-''N''-acetylglucosaminyl)-L-asparaginase
* : glutamin-(asparagin-)ase
* : alkylamidase
* : acylagmatine amidase
* : chitin deacetylase
* : nicotinamide-nucleotide amidase
* : peptidyl-glutaminase
* : protein-glutamine glutaminase
* EC 3.5.1.45: Now listed only as urea carboxylase
* : 6-aminohexanoate-dimer hydrolase
* : N-acetyldiaminopimelate deacetylase, ''N''-acetyldiaminopimelate deacetylase
* : acetylspermidine deacetylase
* : formamidase
* : pentanamidase
* : 4-acetamidobutyryl-CoA deacetylase
* : peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase, peptide-''N''⁴-(''N''-acetyl-β-glucosaminyl)asparagine amidase
* : N-carbamoylputrescine amidase, ''N''-carbamoylputrescine amidase
* : allophanate hydrolase
* : long-chain-fatty-acyl-glutamate deacylase
* : N,N-dimethylformamidase, ''N,N''-dimethylformamidase
* : tryptophanamidase
* : N-benzyloxycarbonylglycine hydrolase, ''N''-benzyloxycarbonylglycine hydrolase
* : N-carbamoylsarcosine amidase, ''N''-carbamoylsarcosine amidase
* : N-(long-chain-acyl)ethanolamine deacylase, ''N''-(long-chain-acyl)ethanolamine deacylase
* : mimosinase
* : acetylputrescine deacetylase
* : 4-acetamidobutyrate deacetylase
* : Nalpha-benzyloxycarbonylleucine hydrolase, ''N''^α-benzyloxycarbonylleucine hydrolase
* : theanine hydrolase
* : 2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase
* : 4-methyleneglutaminase
* : N-formylglutamate deformylase, ''N''-formylglutamate deformylase
* : glycosphingolipid deacylase
* : aculeacin-A deacylase
* : N-feruloylglycine deacylase, ''N''-feruloylglycine deacylase
* : D-benzoylarginine-4-nitroanilide amidase, D-benzoylarginine-4-nitroanilide amidase
* : carnitinamidase
* : chenodeoxycholoyltaurine hydrolase
* : urethanase
* : arylalkyl acylamidase
* : N-carbamoyl-D-amino acid hydrolase, ''N''-carbamoyl-D-amino-acid hydrolase
* : glutathionylspermidine amidase
* : phthalyl amidase
* EC 3.5.1.80: Identical to , ''N''-acetylglucosamine-6-phosphate deacetylase
* : N-acyl-D-amino-acid deacylase, ''N''-acyl-D-amino-acid deacylase
* : N-acyl-D-glutamate deacylase, ''N''-acyl-D-glutamate deacylase
* : N-acyl-D-aspartate deacylase, ''N''-acyl-D-aspartate deacylase
* : biuret amidohydrolase
* : (S)-N-acetyl-1-phenylethylamine hydrolase, (''S'')-''N''-acetyl-1-phenylethylamine hydrolase
* : mandelamide amidase
* : N-carbamoyl-L-amino-acid hydrolase, ''N''-carbamoyl-L-amino-acid hydrolase
* : peptide deformylase
* : N-acetylglucosaminylphosphatidylinositol deacetylase, ''N''-acetylglucosaminylphosphatidylinositol deacetylase
* : adenosylcobinamide hydrolase
* : N-substituted formamide deformylase, ''N''-substituted formamide deformylase
* : pantetheine hydrolase
* : glutaryl-7-aminocephalosporanic-acid acylase
* : gamma-glutamyl-gamma-aminobutyrate hydrolase, γ-glutamyl-γ-aminobutyrate hydrolase
* : N-malonylurea hydrolase, ''N''-malonylurea hydrolase
* : succinylglutamate desuccinylase
* : acyl-homoserine-lactone acylase
* : histone deacetylase
* : fatty acid amide hydrolase
* : (R)-amidase, (''R'')-amidase
* : L-proline amide hydrolase, L-proline amide hydrolase
* : 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase, 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3''H'')-one 5′-monophosphate deformylase
* : N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase, ''N''-acetyl-1-D-myo-inositol-2-amino-2-deoxy-α-D-glucopyranoside deacetylase
* : peptidoglycan-N-acetylglucosamine deacetylase, peptidoglycan-''N''-acetylglucosamine deacetylase
* : chitin disaccharide deacetylase
* : N-formylmaleamate deformylase, ''N''-formylmaleamate deformylase
* : maleamate amidohydrolase
* : UDP-3-O-acyl-N-acetylglucosamine deacetylase, UDP-3-''O''-acyl-''N''-acetylglucosamine deacetylase
* : sphingomyelin deacylase
* : peroxyureidoacrylate/ureidoacrylate amidohydrolase, ureidoacrylate amidohydrolase
* : 2-oxoglutaramate amidase
* : 2'-N-acetylparomamine deacetylase, 2′-''N''-acetylparomamine deacetylase
* : 2-acetyl-6-hydroxyneomycin C deacetylase, 2′′′-acetyl-6′′′-hydroxyneomycin C deacetylase
* : ''N''-acyl-aromatic-L-amino acid amidohydrolase *
* : mycothiol ''S''-conjugate amidase *
* : ureidoglycolate amidohydrolase *
* : 6-aminohexanoate-oligomer endohydrolase *
* : γ-glutamyl hercynylcysteine ''S''-oxide hydrolase *
* : Pup amidohydrolase *
* EC 3.5.1.120: Now , 2-aminomuconate deaminase (2-hydroxymuconate-forming)
* : protein ''N''-terminal asparagine amidohydrolase *
* : protein ''N''-terminal glutamine amidohydrolase *
* : γ-glutamylanilide hydrolase *
* : protein deglycase *
* : ''N'' ²-acetyl-L-2,4-diaminobutanoate deacetylase *
* : oxamate amidohydrolase *
* : jasmonoyl-L-amino acid hydrolase *
* : deaminated glutathione amidase *
* : ''N'' ⁵-(cytidine 5′-diphosphoramidyl)-L-glutamine hydrolase *
* : [amino group carrier protein]-lysine hydrolase *
* : 1-carboxybiuret hydrolase *
* : [amino group carrier protein]-ornithine hydrolase *
* : ''N'' ^α-acyl-L-glutamine aminoacylase *
* : (indol-3-yl)acetyl-L-aspartate hydrolase *
* : ''N'' ⁴-acetylcytidine amidohydrolase *
* : ''N,N′''-diacetylchitobiose non-reducing end deacetylase *
: *No Wikipedia article

3.5.2: In cyclic amides

* : barbiturase
* : dihydropyrimidinase
* : dihydroorotase
* : carboxymethylhydantoinase
* : allantoinase
* : beta-lactamase, β-lactamase
* : imidazolonepropionase
* EC 3.5.2.8: Now included with , β-lactamase
* : 5-oxoprolinase (ATP-hydrolysing)
* : creatininase
* : L-lysine-lactamase, L-lysine-lactamase
* : 6-aminohexanoate-cyclic-dimer hydrolase
* : 2,5-dioxopiperazine hydrolase
* : N-methylhydantoinase (ATP-hydrolysing), ''N''-methylhydantoinase (ATP-hydrolysing)
* : cyanuric acid amidohydrolase
* : maleimide hydrolase
* : hydroxyisourate hydrolase
* : enamidase
* : streptothricin hydrolase
* : isatin hydrolase *
: *No Wikipedia article

3.5.3: In linear amidines

* : arginase
* : guanidinoacetase
* : creatinase
* : allantoicase
* : formimidoylaspartate deiminase
* : arginine deiminase
* : guanidinobutyrase
* : formimidoylglutamase
* : allantoate deiminase
* : D-arginase, D-arginase
* : agmatinase
* : agmatine deiminase
* : formimidoylglutamate deiminase
* : amidinoaspartase
* : protein-arginine deiminase
* : methylguanidinase
* : guanidinopropionase
* : dimethylargininase
* EC 3.5.3.19: Now , ureidoglycolate amidohydrolase
* : diguanidinobutanase
* : Methylenediurea deaminase
* : proclavaminate amidinohydrolase
* : N-succinylarginine dihydrolase, ''N''-succinylarginine dihydrolase
* : ''N'' ¹-aminopropylagmatine ureohydrolase *
* : ''N'' ^ω-hydroxy-L-arginine amidinohydrolase *
* : (''S'')-ureidoglycine aminohydrolase *
: *No Wikipedia article

3.5.4: In cyclic amidines

* : cytosine deaminase
* : adenine deaminase
* : guanine deaminase
* : adenosine deaminase
* : cytidine deaminase
* : AMP deaminase
* : ADP deaminase
* : aminoimidazolase
* : methenyltetrahydrofolate cyclohydrolase
* : IMP cyclohydrolase
* : pterin deaminase
* : dCMP deaminase
* : dCTP deaminase
* EC 3.5.4.14: Now included in , (deoxy)cytidine deaminase
* : guanosine deaminase
* : GTP cyclohydrolase I
* : adenosine-phosphate deaminase
* : ATP deaminase
* : phosphoribosyl-AMP cyclohydrolase
* : pyrithiamine deaminase
* : creatinine deaminase
* : 1-pyrroline-4-hydroxy-2-carboxylate deaminase
* : blasticidin-S deaminase
* : sepiapterin deaminase
* : GTP cyclohydrolase II
* : diaminohydroxyphosphoribosylaminopyrimidine deaminase
* : methenyltetrahydromethanopterin cyclohydrolase
* : S-adenosylhomocysteine deaminase, ''S''-adenosylhomocysteine deaminase
* : GTP cyclohydrolase IIa
* : dCTP deaminase (dUMP-forming)
* : S-methyl-5'-thioadenosine deaminase, ''S''-methyl-5′-thioadenosine deaminase
* : 8-oxoguanine deaminase
* : tRNA(adenine³⁴) deaminase *
* : tRNA^Ala(adenine³⁷) deaminase *
* : tRNA(cytosine⁸) deaminase *
* : mRNA(cytosine⁶⁶⁶⁶) deaminase *
* : double-stranded RNA adenine deaminase *
* : single-stranded DNA cytosine deaminase *
* : GTP cyclohydrolase IV *
* : aminodeoxyfutalosine deaminase *
* : 5'-deoxyadenosine deaminase
* : ''N''-isopropylammelide isopropylaminohydrolase *
* : hydroxydechloroatrazine ethylaminohydrolase *
* : ectoine hydrolase *
* : melamine deaminase *
* : cAMP deaminase *
: *No Wikipedia article

3.5.5: In nitriles

* : nitrilase
* : ricinine nitrilase
* EC 3.5.5.3: Now , cyanate hydratase
* : cyanoalanine nitrilase
* : arylacetonitrilase
* : bromoxynil nitrilase
* : aliphatic nitrilase
* : thiocyanate hydrolase

3.5.99: In other compounds

* : riboflavinase
* : aminopyrimidine aminohydrolase
* EC 3.5.99.3: Now , hydroxydechloroatrazine ethylaminohydrolase
* EC 3.5.99.4: Now , ''N''-isopropylammelide isopropylaminohydrolase
* : 2-aminomuconate deaminase
* : glucosamine-6-phosphate deaminase
* : 1-aminocyclopropane-1-carboxylate deaminase
* : 5-nitroanthranilic acid aminohydrolase
* : 2-nitroimidazole nitrohydrolase
* : 2-iminobutanoate/2-iminopropanoate deaminase *
* : 2-aminomuconate deaminase (2-hydroxymuconate-forming) *
: *No Wikipedia article

EC 3.6: Acid anhydride hydrolases, Acting on acid anhydrides

3.6.1: In phosphorus-containing anhydrides

* : inorganic diphosphatase
* : trimetaphosphatase
* : adenosinetriphosphatase
* EC 3.6.1.4: Now included with , adenosinetriphosphatase
* : apyrase
* : nucleoside-diphosphatase, nucleoside diphosphate phosphatase
* : acylphosphatase
* : ATP diphosphatase
* : nucleotide diphosphatase
* : endopolyphosphatase
* : exopolyphosphatase
* : dCTP diphosphatase
* : ADP-ribose diphosphatase
* : adenosine-tetraphosphatase
* : nucleoside-triphosphatase
* : CDP-glycerol diphosphatase
* : bis(5'-nucleosyl)-tetraphosphatase (asymmetrical), bis(5′-nucleosyl)-tetraphosphatase (asymmetrical))
* : FAD diphosphatase
* EC 3.6.1.19: Now , nucleotide diphosphatase
* : 5'-acylphosphoadenosine hydrolase, 5′-acylphosphoadenosine hydrolase
* : ADP-sugar diphosphatase
* : NAD+ diphosphatase, NAD⁺ diphosphatase
* : dUTP diphosphatase
* : nucleoside phosphoacylhydrolase
* : triphosphatase
* : CDP-diacylglycerol diphosphatase
* : undecaprenyl-diphosphatase
* : thiamine-triphosphatase
* : bis(5'-adenosyl)-triphosphatase, bis(5′-adenosyl)-triphosphatase
* EC 3.6.1.30: Now covered by [m⁷GpppX diphosphatase] and [m⁷GpppN-mRNA hydrolase].
* : phosphoribosyl-ATP diphosphatase
* EC 3.6.1.32: Now , myosin ATPase
* EC 3.6.1.33: Now , dynein ATPase
* EC 3.6.1.34: Transferred entry: H⁺-transporting ATP synthase. Now , H⁺-transporting two-sector ATPase
* EC 3.6.1.35: Now , H⁺-exporting ATPase
* EC 3.6.1.36: Now , H⁺/K⁺-exchanging ATPase
* EC 3.6.1.37: Now , Na⁺/K⁺-exchanging ATPase
* EC 3.6.1.38: Now , Ca²⁺-transporting ATPase
* : thymidine-triphosphatase
* : guanosine-5'-triphosphate,3'-diphosphate diphosphatase, guanosine-5′-triphosphate,3′-diphosphate phosphatase
* : bis(5'-nucleosyl)-tetraphosphatase (symmetrical), bis(5′-nucleosyl)-tetraphosphatase (symmetrical)
* : guanosine-diphosphatase
* : dolichyldiphosphatase
* : oligosaccharide-diphosphodolichol diphosphatase
* : UDP-sugar diphosphatase
* EC 3.6.1.46: Now , heterotrimeric G-protein GTPase
* EC 3.6.1.47: Now , protein-synthesizing GTPase
* EC 3.6.1.49: Now , signal-recognition-particle GTPase
* EC 3.6.1.50: Now , dynamin GTPase
* EC 3.6.1.51: Now , tubulin GTPase
* : diphosphoinositol-polyphosphate diphosphatase
* : Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase, Mn²⁺-dependent ADP-ribose/CDP-alcohol diphosphatase
* : UDP-2,3-diacylglucosamine diphosphatase
* : 8-oxo-dGTP diphosphatase
* : 2-hydroxy-dATP diphosphatase
* : UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase, UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose hydrolase
* : 8-oxo-dGDP phosphatase
* : m7GpppX diphosphatase, 5′-(N⁷-methyl 5′-triphosphoguanosine)-[mRNA] diphosphatase
* : diadenosine hexaphosphate hydrolase (AMP-forming)
* : diadenosine hexaphosphate hydrolase (ATP-forming)
* : m7GpppN-mRNA hydrolase, 5′-(N⁷-methylguanosine 5′-triphospho)-[mRNA] hydrolase
* : alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, α-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase
* : inosine diphosphate phosphatase *
* : (d)CTP diphosphatase *
* : XTP/dITP diphosphatase *
* : dihydroneopterin triphosphate diphosphatase *
* : geranyl diphosphate phosphohydrolase *
* : 8-oxo-(d)GTP phosphatase *
* : guanosine-5′-diphospho-5′- NAdiphosphatase *
* : adenosine-5′-diphospho-5′- NAdiphosphatase *
* : DNA-3′-diphospho-5′-guanosine diphosphatase *
* : inosine/xanthosine triphosphatase *
* : mRNA 5′-phosphatase *
: *No Wikipedia article

3.6.2: In sulfonyl-containing anhydrides

* : adenylylsulfatase
* : phosphoadenylylsulfatase

3.6.3: Acting on acid anhydrides to catalyse transmembrane movement of substances

* : phospholipid-translocating ATPase
* EC 3.6.3.2: Now , P-type Mg²⁺ transporter
* EC 3.6.3.3: Now , Cd²⁺-exporting ATPase
* EC 3.6.3.4: Now , Cu²⁺-exporting ATPase
* EC 3.6.3.5: Now , Zn²⁺-exporting ATPase
* EC 3.6.3.6: Now , P-type H⁺-exporting transporter
* EC 3.6.3.7: Now , P-type Na⁺ transporter
* EC 3.6.3.8: Now , Ca²⁺-transporting ATPase
* EC 3.6.3.9: Now , Na⁺/K⁺-exchanging ATPase
* EC 3.6.3.10: Now , H⁺/K⁺-exchanging ATPase
* EC 3.6.3.11: Cl^–-transporting ATPase. The activity was only ever studied in crude extracts, and is an artifact
* EC 3.6.3.12: Now , K⁺-transporting ATPase
* EC 3.6.3.13:Identical to , phospholipid-translocating ATPase
* EC 3.6.3.14: Now , H⁺-transporting two-sector ATPase
* EC 3.6.3.15: Now , Na⁺-transporting two-sector ATPase
* EC 3.6.3.16: Now , arsenite-transporting ATPase
* EC 3.6.3.17: Now covered by various ABC-type monosaccharide transporters in sub-subclass EC 7.5.2
* EC 3.6.3.18: Now , ABC-type oligosaccharide transporter
* EC 3.6.3.19: Now , ABC-type maltose transporter
* EC 3.6.3.20: Now , glycerol-3-phosphate-transporting ATPase
* EC 3.6.3.21: Now , ABC-type polar-amino-acid transporter
* EC 3.6.3.22: Now , ABC-type nonpolar-amino-acid transporter
* EC 3.6.3.23: Now , oligopeptide-transporting ATPase
* EC 3.6.3.24: Now , nickel-transporting ATPase
* EC 3.6.3.25: Now , sulfate-transporting ATPase
* EC 3.6.3.26: Now , nitrate-transporting ATPase
* EC 3.6.3.27: Now , ABC-type phosphate transporter
* EC 3.6.3.28: Now , ABC-type phosphonate transporter
* EC 3.6.3.29: Now , molybdate-transporting ATPase
* EC 3.6.3.30: Now , Fe³⁺-transporting ATPase
* EC 3.6.3.31: Now , polyamine-transporting ATPase
* EC 3.6.3.32: Now , quaternary-amine-transporting ATPase
* EC 3.6.3.33: Now , vitamin B₁₂-transporting ATPase
* EC 3.6.3.34: now recognized to be at least three separate enzymes , iron(III) hydroxamate ABC transporter, , ferric enterobactin ABC transporter, and , ferric citrate ABC transporter
* EC 3.6.3.35: Now , manganese-transporting ATPase
* EC 3.6.3.36: Now , taurine-transporting ATPase
* EC 3.6.3.37: Now , guanine-transporting ATPase
* EC 3.6.3.38: Now , ABC-type capsular-polysaccharide transporter
* EC 3.6.3.39: Now , lipopolysaccharide-transporting ATPase
* EC 3.6.3.40: Now , teichoic-acid-transporting ATPase
* EC 3.6.3.41: Now , heme-transporting ATPase
* EC 3.6.3.42: Now , β-glucan-transporting ATPase
* EC 3.6.3.43: Now , peptide-transporting ATPase
* EC 3.6.3.44: Now , ABC-type xenobiotic transporter
* EC 3.6.3.45: Now included with , xenobiotic-transporting ATPase
* EC 3.6.3.46: Now , ABC-type Cd²⁺ transporter
* EC 3.6.3.47: Now , fatty-acyl-CoA-transporting ATPase
* EC 3.6.3.48: Now as α-factor-pheromone transporting ATPase
* EC 3.6.3.49: Now , channel-conductance-controlling ATPase
* EC 3.6.3.50: Now , protein-secreting ATPase
* EC 3.6.3.51: Now , mitochondrial protein-transporting ATPase
* EC 3.6.3.52: Now , chloroplast protein-transporting ATPase
* EC 3.6.3.53: Now , Ag⁺-exporting ATPase
* EC 3.6.3.54: Now , Cu⁺-exporting ATPase
* EC 3.6.3.55: Now , tungstate-importing ATPase

3.6.4: Acting on acid anhydrides to facilitate cellular and subcellular movement

* EC 3.6.4.1: Now , myosin ATPase
* EC 3.6.4.2: Now , dynein ATPase
* EC 3.6.4.3: Now , microtubule-severing ATPase
* EC 3.6.4.4: Now , plus-end-directed kinesin ATPase
* EC 3.6.4.5: Now , minus-end-directed kinesin ATPase
* : vesicle-fusing ATPase
* : peroxisome-assembly ATPase
* EC 3.6.4.8: Now , proteasome ATPase
* EC 3.6.4.9: Now , chaperonin ATPase
* : non-chaperonin molecular chaperone ATPase
* EC 3.6.4.11: Deleted, the activity has been shown not to take place
* : DNA helicase
* : RNA helicase

3.6.5: Acting on GTP to facilitate cellular and subcellular movement

* : heterotrimeric G-protein GTPase
* : small monomeric GTPase
* : protein-synthesizing GTPase
* : signal-recognition-particle GTPase
* : dynamin GTPase
* : tubulin GTPase

EC 3.7: Acting on carbon-carbon bonds

EC 3.7.1: In ketonic substances

* : oxaloacetase
* : fumarylacetoacetase
* : kynureninase
* : phloretin hydrolase
* : acylpyruvate hydrolase
* : acetylpyruvate hydrolase
* : beta-diketone hydrolase, β-diketone hydrolase
* : 2,6-dioxo-6-phenylhexa-3-enoate hydrolase
* : 2-hydroxymuconate-semialdehyde hydrolase
* : cyclohexane-1,3-dione hydrolase
* : cyclohexane-1,2-dione hydrolase
* : cobalt-precorrin 5A hydrolase
* : 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase
* : 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
* EC 3.7.1.15: Now , (+)-caryolan-1-ol synthase
* EC 3.7.1.16: Now , oxepin-CoA hydrolase
* : 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
* : 6-oxocamphor hydrolase
* : 2,6-dihydroxypseudooxynicotine hydrolase
* : 3-fumarylpyruvate hydrolase
* : 6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase *
* : 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione acylhydrolase (ring-opening)
* : maleylpyruvate hydrolase *
* : 2,4-diacetylphloroglucinol hydrolase *
* : 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase *
* : 2,4-didehydro-3-deoxy-L-rhamnonate hydrolase *
* : neryl diphosphate diphosphatase *
* : 3-oxoisoapionate-4-phosphate transcarboxylase/hydrolase *
: *No Wikipedia article

EC 3.8: Acting on halide bonds

EC 3.8.1: Acting on halide bonds

* EC 3.8.1.1: Covered by , haloalkane dehalogenase.
* : 2-haloacid dehalogenase, (''S'')-2-haloacid dehalogenase
* : haloacetate dehalogenase
* EC 3.8.1.4: Now , thyroxine 5′-deiodinase
* : haloalkane dehalogenase
* : 4-chlorobenzoate dehalogenase
* : 4-chlorobenzoyl-CoA dehalogenase
* : atrazine chlorohydrolase
* : (R)-2-haloacid dehalogenase, (''R'')-2-haloacid dehalogenase
* : 2-haloacid dehalogenase (configuration-inverting)
* : 2-haloacid dehalogenase (configuration-retaining)

3.8.2: In phosphorus-halide compounds (deleted sub-subclass)

* EC 3.8.2.1: Now , diisopropyl-fluorophosphatase

EC 3.9: act on phosphorus-nitrogen bonds

EC 3.9.1: Acting on phosphorus-nitrogen bonds (only sub-subclass identified to date)

* : phosphoamidase
* : protein arginine phosphatase *
* : phosphohistidine phosphatase *
: *No Wikipedia article

EC 3.10: Acting on sulfur-nitrogen bonds

EC 3.10.1: Acting on sulfur-nitrogen bonds (only sub-subclass identified to date)

* : N-sulfoglucosamine sulfohydrolase, ''N''-sulfoglucosamine sulfohydrolase
* : cyclamate sulfohydrolase

EC 3.11: Acting on carbon-phosphorus bonds

EC 3.11.1: Acting on carbon-phosphorus bonds (only sub-subclass identified to date)

* : phosphonoacetaldehyde hydrolase
* : phosphonoacetate hydrolase
* : phosphonopyruvate hydrolase

EC 3.12: Acting on sulfur-sulfur bonds

EC 3.12.1: Acting on sulfur-sulfur bonds (only sub-subclass identified to date)

* : trithionate hydrolase

EC 3.13: Acting on carbon-sulfur bonds

EC 3.13.1: Acting on carbon-sulfur bonds (only sub-subclass identified to date)

* : UDP-sulfoquinovose synthase
* EC 3.13.1.2: Deleted, the activity is most probably attributable to , ''S''-ribosylhomocysteine lyase
* : 2'-hydroxybiphenyl-2-sulfinate desulfinase, 2′-hydroxybiphenyl-2-sulfinate desulfinase
* : 3-sulfinopropanoyl-CoA desulfinase *
* : carbon disulfide hydrolase
* : [CysO sulfur-carrier protein]-''S''-L-cysteine hydrolase *
* : Carbonyl sulfide hydrolase
* : ''S''-adenosyl-L-methionine hydrolase (adenosine-forming *
* : ''S''-inosyl-L-homocysteine hydrolase *
: *No Wikipedia article

References

{{Reflist

Hydrolases, *
Biology-related lists, EC3