Bulb Pipette
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A volumetric pipette, bulb pipette, or belly pipette allows extremely accurate measurement (to four significant figures) of the volume of a solution. It is calibrated to deliver accurately a fixed volume of liquid. These
pipette A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing ...
s have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a single volume (like a
volumetric flask A volumetric flask (measuring flask or graduated flask) is a piece of laboratory apparatus, a type of laboratory flask, calibrated to contain a precise volume at a certain temperature. Volumetric flasks are used for precise dilutions and prepar ...
). Typical volumes are 1, 2, 5, 10, 20, 25, 50 and 100 mL. Volumetric pipettes are commonly used in
analytical chemistry Analytical skill, Analytical chemistry studies and uses instruments and methods to Separation process, separate, identify, and Quantification (science), quantify matter. In practice, separation, identification or quantification may constitute t ...
to make laboratory solutions from a base stock as well as to prepare solutions for
titration Titration (also known as titrimetry and volumetric analysis) is a common laboratory method of Quantitative research, quantitative Analytical chemistry, chemical analysis to determine the concentration of an identified analyte (a substance to be ...
.
ASTM ASTM International, formerly known as American Society for Testing and Materials, is a standards organization that develops and publishes voluntary consensus technical international standards for a wide range of materials, products, systems and s ...
standard E969 defines the standard tolerance for volumetric transfer pipettes. The tolerance depends on the size: a 0.5-mL pipette has a tolerance of ±0.006 mL, while a 50-mL pipette has a tolerance of ±0.05 mL. (These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels.


History

Pyrex Pyrex (trademarked as ''PYREX'' and ''pyrex'') is a brand introduced by Corning Inc. in 1915, initially for a line of clear, low-thermal-expansion borosilicate glass used for laboratory glassware and kitchenware. It was later expanded in the 1 ...
started to make laboratory equipment in 1916 and became a favorite brand for the scientific community due to the
borosilicate glass Borosilicate glass is a type of glass with silica and boron trioxide as the main glass-forming constituents. Borosilicate glasses are known for having very low coefficients of thermal expansion (≈3 × 10−6 K−1 at 20 °C), ma ...
's natural properties. These included strength against; chemicals, thermal shift, and mechanical stress.


References


External links


Helpful Hints on the Use of a Volumetric Pipet
by Oliver Seely Laboratory glassware Laboratory equipment Analytical chemistry Volumetric instruments {{analytical-chemistry-stub