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Heat Fixation
In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction. It terminates any ongoing biochemical reactions and may also increase the treated tissues' mechanical strength or stability. Tissue fixation is a critical step in the preparation of histological sections, its broad objective being to preserve cells and tissue components and to do this in such a way as to allow for the preparation of thin, stained sections. This allows the investigation of the tissues' structure, which is determined by the shapes and sizes of such macromolecules (in and around cells) as proteins and nucleic acids. Purposes In performing their protective role, fixatives denature proteins by coagulation, by forming additive compounds, or by a combination of coagulation and additive processes. A compound that adds chemically to macromolecules stabilizes structure most effectively if it is able to combine with parts ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Histology
Histology, also known as microscopic anatomy or microanatomy, is the branch of biology that studies the microscopic anatomy of biological tissue (biology), tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope. Although one may divide microscopic anatomy into ''organology'', the study of organs, ''histology'', the study of tissues, and ''cytology'', the study of cell (biology), cells, modern usage places all of these topics under the field of histology. In medicine, histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue. In the field of paleontology, the term paleohistology refers to the histology of fossil organisms. Biological tissues Animal tissue classification There are four basic types of animal tissues: muscle tissue, nervous tissue, connective tissue, and epithelial tissue. All animal tissues are considered to be subtypes of these ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram-positive Bacteria
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is used by microbiologists to place bacteria into two main categories, gram-positive (+) and gram-negative (−). Gram-positive bacteria have a thick layer of peptidoglycan within the cell wall, and gram-negative bacteria have a thin layer of peptidoglycan. Gram-positive bacteria retain the crystal violet stain used in the test, resulting in a purple color when observed through an optical microscope. The thick layer of peptidoglycan in the bacterial cell wall retains the stain after it has been fixed in place by iodine. During the decolorization step, the decolorizer removes crystal violet from all other cells. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Atrium (heart)
The atrium (; : atria) is one of the two Heart#Chambers, upper chambers in the heart that receives blood from the circulatory system. The blood in the atria is pumped into the Ventricle (heart), heart ventricles through the atrioventricular valve, atrioventricular mitral valve, mitral and tricuspid valve, tricuspid heart valves. There are two atria in the human heart – the left atrium receives blood from the pulmonary circulation, and the right atrium receives blood from the venae cavae of the systemic circulation. During the cardiac cycle, the atria receive blood while relaxed in diastole, then contract in systole to move blood to the ventricles. Each atrium is roughly cube-shaped except for an ear-shaped projection called an atrial appendage, previously known as an auricle. All animals with a closed circulatory system have at least one atrium. The atrium was formerly called the 'auricle'. That term is still used to describe this chamber in some other animals, such as the ''Mo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ventricle (heart)
A ventricle is one of two large chambers located toward the bottom of the heart that collect and expel blood towards the peripheral beds within the body and lungs. The blood pumped by a ventricle is supplied by an atrium, an adjacent chamber in the upper heart that is smaller than a ventricle. Interventricular means between the ventricles (for example the interventricular septum), while intraventricular means within one ventricle (for example an intraventricular block). In a four-chambered heart, such as that in humans, there are two ventricles that operate in a double circulatory system: the right ventricle pumps blood into the pulmonary circulation to the lungs, and the left ventricle pumps blood into the systemic circulation through the aorta. Structure Ventricles have thicker walls than atria and generate higher blood pressures. The physiological load on the ventricles requiring pumping of blood throughout the body and lungs is much greater than the pressure generated by ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Perfusion Diagram
Perfusion is the passage of fluid through the circulatory system or lymphatic system to an organ or a tissue, usually referring to the delivery of blood to a capillary bed in tissue. Perfusion may also refer to fixation via perfusion, used in histological studies. Perfusion is measured as the rate at which blood is delivered to tissue, or volume of blood per unit time (blood flow) per unit tissue mass. The SI unit is m3/(s·kg), although for human organs perfusion is typically reported in ml/min/g. The word is derived from the French verb ''perfuser'', meaning to "pour over or through". All animal tissues require an adequate blood supply for health and life. Poor perfusion (malperfusion), that is, ischemia, causes health problems, as seen in cardiovascular disease, including coronary artery disease, cerebrovascular disease, peripheral artery disease, and many other conditions. Tests verifying that adequate perfusion exists are a part of a patient's assessment process that are ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Glycocalyx
The glycocalyx (: glycocalyces or glycocalyxes), also known as the pericellular matrix and cell coat, is a layer of glycoproteins and glycolipids which surround the cell membranes of bacteria, epithelial cells, and other cells. Animal epithelial cells have a fuzz-like coating on the external surface of their plasma membranes. This viscous coating is the glycocalyx that consists of several carbohydrate moieties of membrane glycolipids and glycoproteins, which serve as backbone molecules for support. Generally, the carbohydrate portion of the glycolipids found on the surface of plasma membranes helps these molecules contribute to cell–cell recognition, communication, and intercellular adhesion. The glycocalyx is a type of identifier that the body uses to distinguish between its own healthy cells and transplanted tissues, diseased cells, or invading organisms. Included in the glycocalyx are cell-adhesion molecules that enable cells to adhere to each other and guide the mo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Stain
A stain is a discoloration that can be clearly distinguished from the surface, material, or medium it is found upon. They are caused by the chemical or physical interaction of two dissimilar materials. Accidental staining may make materials appear used, degraded or Cleanliness, permanently unclean. Intentional staining is used in biochemical research, and for artistic effect, such as in wood staining, weathering steel, rust staining and stained glass. Types There can be intentional stains (such as wood stains or paint), indicative stains (such as food coloring dye, and staining, the use of one or more substances to enhance visibility of samples in a microscope or other imaging device. Numerous naturally-occurring stains exist, such as rust on iron and a patina on bronze, as do accidental stains such as from ketchup and oil on fabrics and other materials. Different types of material can be stained by different substances, and stain resistance is an important characteristic in mo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Micro-incineration
Micro-incineration or microincineration is a technique to determine the manner and distribution of mineral elements in biological cells, biological tissues and organs. Slide preparation of tissues can be used. Examples include calcium (Ca), potassium (K), sodium (Na), magnesium (Mg), iron (Fe), and silicon (Si). The organic matter is vaporised by heating. The nature and position of the mineral ash is determined microscopically. Aqueous or cryo-EM fixed tissue materials can also be examined under transmission and scanning electron microscopy (TEM & SEM). The ashing procedure produces cellular oxidised-residues rich in Sodium silicate, Na2O, CaO, Magnesium oxide, MgO, Fe2O3, SiO2, Tricalcium phosphate, Ca(PO4)2, Mg(PO4)2, etc., which are detected by X-ray microanalysis with 2-4 times sensitivity gained after incineration of sample, due to increased mineral concentration and reduced nonspecific background radiation. See more http://jcb.rupress.org/content/39/1/55.full.pdf Bi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bunsen Burner
A Bunsen burner, named after Robert Bunsen, is a kind of ambient air gas burner used as laboratory equipment; it produces a single open gas flame, and is used for heating, sterilization, and combustion. The gas can be natural gas (which is mainly methane) or a liquefied petroleum gas, such as propane, butane, a mixture or, as Bunsen himself used, coal gas. Combustion temperature achieved depends in part on the adiabatic flame temperature of the chosen fuel mixture. History In 1852, the University of Heidelberg hired Bunsen and promised him a new laboratory building. The city of Heidelberg had begun to install coal-gas street lighting, and the university laid gas lines to the new laboratory. The designers of the building intended to use the gas not just for lighting, but also as fuel for burners for laboratory operations. For any burner lamp, it was desirable to maximize the temperature of its flame, and minimize its luminosity (which represented lost heating energy). Bunsen ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microscope Slide
A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope. Typically the object is mounted (secured) on the slide, and then both are inserted together in the microscope for viewing. This arrangement allows several slide-mounted objects to be quickly inserted and removed from the microscope, labeled, transported, and stored in appropriate slide cases or folders etc. Microscope slides are often used together with a cover slip or cover glass, a smaller and thinner sheet of glass that is placed over the specimen. Slides are held in place on the microscope's stage by slide clips, slide clamps or a cross-table which is used to achieve precise, remote movement of the slide upon the microscope's stage (such as in an automated/computer operated system, or where touching the slide with fingers is inappropriate either due to the risk of contamination or lack of precisio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Archaea
Archaea ( ) is a Domain (biology), domain of organisms. Traditionally, Archaea only included its Prokaryote, prokaryotic members, but this has since been found to be paraphyletic, as eukaryotes are known to have evolved from archaea. Even though the domain Archaea Cladistics, cladistically includes eukaryotes, the term "archaea" (: archaeon , from the Greek "ἀρχαῖον", which means ancient) in English still generally refers specifically to prokaryotic members of Archaea. Archaea were initially Taxonomy (biology), classified as bacteria, receiving the name archaebacteria (, in the Archaebacteria Kingdom (biology), kingdom), but this term has fallen out of use. Archaeal cells have unique properties separating them from Bacteria and Eukaryote, Eukaryota. Archaea are further divided into multiple recognized phylum, phyla. Classification is difficult because most have not been Isolation (microbiology), isolated in a laboratory and have been detected only by their Gene, gene s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
MDA-MB 231
Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease. Experiments using cell culture Cell culture or tissue culture is the process by which cell (biology), cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been Cell isolation, isolated from living tissue, ... are used for developing new diagnostic tests and new treatments for diseases. This is a list of major breast cancer cell lines that are primarily used in breast cancer research. List of cell lines Notes References {{DEFAULTSORT:Breast Cancer Cell Lines * * ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
