Microscopy
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Microscopy
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the obje ...
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Transmission Electron Microscope
Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The image is then magnified and focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a sensor such as a scintillator attached to a charge-coupled device. Transmission electron microscopes are capable of imaging at a significantly higher resolution than light microscopes, owing to the smaller de Broglie wavelength of electrons. This enables the instrument to capture fine detail—even as small as a single column of atoms, which is thousands of times smaller than a resolvable object seen in a light microscope. Transmission electron microscopy is a major analytical method i ...
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X-ray Microscopy
An X-ray microscope uses electromagnetic radiation in the soft X-ray band to produce magnified images of objects. Since X-rays penetrate most objects, there is no need to specially prepare them for X-ray microscopy observations. Unlike visible light, X-rays do not reflect or refract easily and are invisible to the human eye. Therefore, an X-ray microscope exposes film or uses a charge-coupled device (CCD) detector to detect X-rays that pass through the specimen. It is a contrast imaging technology using the difference in absorption of soft X-rays in the water window region (wavelengths: 2.34–4.4 nm, energies: 280–530 eV) by the carbon atom (main element composing the living cell) and the oxygen atom (an element of water). Microfocus X-ray also achieves high magnification by projection. A microfocus X-ray tube produces X-rays from an extremely small focal spot (5 μm down to 0.1 μm). The X-rays are in the more conventional X-ray range (20 to 300 keV) ...
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Misc Pollen Colorized
Misc or MISC may refer to: * Misc (title), a gender neutral title * MISC Berhad, or Malaysia International Shipping Corporation * Minimal instruction set computer, a processor architecture * Moi International Sports Centre, in Kasarani, Kenya * Multisystem inflammatory syndrome in children Multisystem inflammatory syndrome in children (MIS-C), or paediatric inflammatory multisystem syndrome (PIMS / PIMS-TS), or systemic inflammatory syndrome in COVID-19 (SISCoV), is a rare systemic illness involving persistent fever and extreme ..., a post-infectious disease associated with COVID-19 See also * * * * Miscellaneous {{disambiguation ...
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Histology
Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope. Although one may divide microscopic anatomy into ''organology'', the study of organs, ''histology'', the study of tissues, and ''cytology'', the study of cells, modern usage places all of these topics under the field of histology. In medicine, histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue. In the field of paleontology, the term paleohistology refers to the histology of fossil organisms. Biological tissues Animal tissue classification There are four basic types of animal tissues: muscle tissue, nervous tissue, connective tissue, and epithelial tissue. All animal tissues are considered to be subtypes of these four principal tissue type ...
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Cornelis Drebbel
Cornelis Jacobszoon Drebbel ( ) (1572 – 7 November 1633) was a Dutch engineer and inventor. He was the builder of the first operational submarine in 1620 and an innovator who contributed to the development of measurement and control systems, optics and chemistry. Biography Cornelis Drebbel was born in Alkmaar, Holland in an Anabaptist family in 1572. After some years at the Latin school in Alkmaar, around 1587, he attended the Academy in Haarlem, also located in North-Holland. Teachers at the Academy were Hendrik Goltzius, engraver, painter, alchemist and humanist, Karel van Mander, painter, writer, humanist and Cornelis Corneliszoon of Haarlem. Drebbel became a skilled engraver on copperplate and also took an interest in alchemy. In 1595 he married Sophia Jansdochter Goltzius, younger sister of Hendrick, and settled at Alkmaar. They had at least six children, of whom four survived. Drebbel worked initially as a painter, engraver and cartographer. But he was in constant need ...
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Galileo Galilei
Galileo di Vincenzo Bonaiuti de' Galilei (15 February 1564 – 8 January 1642) was an Italian astronomer, physicist and engineer, sometimes described as a polymath. Commonly referred to as Galileo, his name was pronounced (, ). He was born in the city of Pisa, then part of the Duchy of Florence. Galileo has been called the "father" of observational astronomy, modern physics, the scientific method, and modern science. Galileo studied speed and velocity, gravity and free fall, the principle of relativity, inertia, projectile motion and also worked in applied science and technology, describing the properties of pendulums and " hydrostatic balances". He invented the thermoscope and various military compasses, and used the telescope for scientific observations of celestial objects. His contributions to observational astronomy include telescopic confirmation of the phases of Venus, observation of the four largest satellites of Jupiter, observation of Saturn's rin ...
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Compound Microscope
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope. In high-power microscopes, both eyepieces typically show the same image, but with a stereo microscope, slightly different images are used to create a 3-D effect. A camera is typically used to capture the image (micrograph). The sample can be lit in a variety of ways. Transparent objects can be lit from below and solid objects can be lit with light coming through ( bright field) or around ( dark field) the objective lens. Polar ...
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Eyeglasses
Glasses, also known as eyeglasses or spectacles, are vision eyewear, with lenses (clear or tinted) mounted in a frame that holds them in front of a person's eyes, typically utilizing a bridge over the nose and hinged arms (known as temples or temple pieces) that rest over the ears. Glasses are typically used for vision correction, such as with reading glasses and glasses used for nearsightedness; however, without the specialized lenses, they are sometimes used for cosmetic purposes. Safety glasses provide eye protection against flying debris for construction workers or lab technicians; these glasses may have protection for the sides of the eyes as well as in the lenses. Some types of safety glasses are used to protect against visible and near-visible light or radiation. Glasses are worn for eye protection in some sports, such as squash. Glasses wearers may use a strap to prevent the glasses from falling off. Wearers of glasses that are used only part of the time may have t ...
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Magnifying Glass
A magnifying glass is a convex lens that is used to produce a magnified image of an object. The lens is usually mounted in a frame with a handle. A magnifying glass can be used to focus light, such as to concentrate the sun's radiation to create a hot spot at the focus for fire starting. A sheet magnifier consists of many very narrow concentric ring-shaped lenses, such that the combination acts as a single lens but is much thinner. This arrangement is known as a Fresnel lens. The magnifying glass is an icon of detective fiction, particularly that of Sherlock Holmes. History "The evidence indicates that the use of lenses was widespread throughout the Middle East and the Mediterranean basin over several millennia". The earliest explicit written evidence of a magnifying device is a joke in Aristophanes's '' The Clouds'' from 424 BC, where magnifying lenses to ignite tinder were sold in a pharmacy, and Pliny the Elder's "lens", a glass globe filled with water, used to ...
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Lens (optics)
A lens is a transmissive optical device which focuses or disperses a light beam by means of refraction. A simple lens consists of a single piece of transparent material, while a compound lens consists of several simple lenses (''elements''), usually arranged along a common axis. Lenses are made from materials such as glass or plastic, and are ground and polished or molded to a desired shape. A lens can focus light to form an image, unlike a prism, which refracts light without focusing. Devices that similarly focus or disperse waves and radiation other than visible light are also called lenses, such as microwave lenses, electron lenses, acoustic lenses, or explosive lenses. Lenses are used in various imaging devices like telescopes, binoculars and cameras. They are also used as visual aids in glasses to correct defects of vision such as myopia and hypermetropia. History The word ''lens'' comes from '' lēns'', the Latin name of the lentil (a seed of a lent ...
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Optical Microscopy
Optics is the branch of physics that studies the behaviour and properties of light, including its interactions with matter and the construction of instruments that use or detect it. Optics usually describes the behaviour of visible, ultraviolet, and infrared light. Because light is an electromagnetic wave, other forms of electromagnetic radiation such as X-rays, microwaves, and radio waves exhibit similar properties. Most optical phenomena can be accounted for by using the classical electromagnetic description of light. Complete electromagnetic descriptions of light are, however, often difficult to apply in practice. Practical optics is usually done using simplified models. The most common of these, geometric optics, treats light as a collection of rays that travel in straight lines and bend when they pass through or reflect from surfaces. Physical optics is a more comprehensive model of light, which includes wave effects such as diffraction and interference that cannot b ...
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Anthonie Van Leeuwenhoek (1632-1723)
Antonie Philips van Leeuwenhoek ( ; ; 24 October 1632 – 26 August 1723) was a Dutch microbiologist and microscopist in the Golden Age of Dutch science and technology. A largely self-taught man in science, he is commonly known as " the Father of Microbiology", and one of the first microscopists and microbiologists. Van Leeuwenhoek is best known for his pioneering work in microscopy and for his contributions toward the establishment of microbiology as a scientific discipline. Raised in Delft, Dutch Republic, van Leeuwenhoek worked as a draper in his youth and founded his own shop in 1654. He became well recognized in municipal politics and developed an interest in lensmaking. In the 1670s, he started to explore microbial life with his microscope. This was one of the notable achievements of the Golden Age of Dutch exploration and discovery (). Using single-lensed microscopes of his own design and make, van Leeuwenhoek was the first to observe and to experiment with mic ...
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