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Flow Cytometry
Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: * Cell counting * Cell sorting * Determining cell characteristics and function * Detecting microorganisms * Biomarker detection * Protein engineering detection * Diagnosis of health disorders such as blood cancers * Me ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cytometry
Cytometry is the measurement of number and characteristics of cell (biology), cells. Variables that can be measured by cytometric methods include cell size, cell counting, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the cytoplasm. Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count. In a similar fashion, cytometry is also used in cell biology research and in medical diagnostics to characterize cells in a wide range of applications associated with diseases such as cancer and AIDS. Cytometric devices Image cytometers Image cytometry is the oldest form of cytometry. Image cytometers operate by statically imaging a large number of cells using optical microscopy. Prior to analysis, cells are commonly stained to enhance contrast or to detect specific molecules by labeling these with fluorochromes. Traditionally, cel ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Absorption (electromagnetic Radiation)
In physics, absorption of electromagnetic radiation is how matter (typically electrons bound in atoms) takes up a photon's energy—and so transforms electromagnetic energy into internal energy of the absorber (for example, thermal energy). A notable effect of the absorption of electromagnetic radiation is attenuation of the radiation; attenuation is the gradual reduction of the intensity of light waves as they propagate through a medium. Although the absorption of waves does not usually depend on their intensity (linear absorption), in certain conditions (optics) the medium's transparency changes by a factor that varies as a function of wave intensity, and saturable absorption (or nonlinear absorption) occurs. Quantifying absorption Many approaches can potentially quantify radiation absorption, with key examples following. * The absorption coefficient along with some closely related derived quantities * The attenuation coefficient (NB used infrequently with meaning ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Krypton Laser
An ion laser is a gas laser that uses an ionized gas as its lasing medium. Like other gas lasers, ion lasers feature a sealed cavity containing the laser medium and mirrors forming a Fabry–Pérot resonator. Unlike helium–neon lasers, the energy level transitions that contribute to laser action come from ions. Because of the large amount of energy required to excite the ionic transitions used in ion lasers, the required current is much greater, and as a result almost all except for the smallest ion lasers are water-cooled. A small air-cooled ion laser might produce, for example, 130 milliwatts of output light with a tube current of about 10 amperes and a voltage of 105 volts. Since one ampere times one volt is one watt, this is an electrical power input of about one kilowatt. Subtracting the (desirable) light output of 130 mW from power input, this leaves the large amount of waste heat of nearly one kW. This has to be dissipated by the cooling system. In other words, the p ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Xenon
Xenon is a chemical element; it has symbol Xe and atomic number 54. It is a dense, colorless, odorless noble gas found in Earth's atmosphere in trace amounts. Although generally unreactive, it can undergo a few chemical reactions such as the formation of xenon hexafluoroplatinate, the first noble gas compound to be synthesized. Xenon is used in flash lamps and arc lamps, and as a general anesthetic. The first excimer laser design used a xenon dimer molecule (Xe2) as the lasing medium, and the earliest laser designs used xenon flash lamps as pumps. Xenon is also used to search for hypothetical weakly interacting massive particles and as a propellant for ion thrusters in spacecraft. Naturally occurring xenon consists of seven stable isotopes and two long-lived radioactive isotopes. More than 40 unstable xenon isotopes undergo radioactive decay, and the isotope ratios of xenon are an important tool for studying the early history of the Solar System. Radioactive xe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mercury (element)
Mercury is a chemical element; it has Symbol (chemistry), symbol Hg and atomic number 80. It is commonly known as quicksilver. A Heavy metal element, heavy, silvery d-block element, mercury is the only metallic element that is known to be liquid at standard temperature and pressure; the only other element that is liquid under these conditions is the halogen bromine, though metals such as caesium, gallium, and rubidium melt just above room temperature. Mercury occurs in deposits throughout the world mostly as cinnabar (mercuric sulfide). The red pigment vermilion is obtained by Mill (grinding), grinding natural cinnabar or synthetic mercuric sulfide. Exposure to mercury and mercury-containing organic compounds is toxic to the nervous system, immune system and kidneys of humans and other animals; mercury poisoning can result from exposure to water-soluble forms of mercury (such as mercuric chloride or methylmercury) either directly or through mechanisms of biomagnification. Mercu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biological Tissue
In biology, tissue is an assembly of similar cells and their extracellular matrix from the same embryonic origin that together carry out a specific function. Tissues occupy a biological organizational level between cells and a complete organ. Accordingly, organs are formed by the functional grouping together of multiple tissues. The English word "tissue" derives from the French word "", the past participle of the verb tisser, "to weave". The study of tissues is known as histology or, in connection with disease, as histopathology. Xavier Bichat is considered as the "Father of Histology". Plant histology is studied in both plant anatomy and physiology. The classical tools for studying tissues are the paraffin block in which tissue is embedded and then sectioned, the histological stain, and the optical microscope. Developments in electron microscopy, immunofluorescence, and the use of frozen tissue-sections have enhanced the detail that can be observed in tissues. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Quantification (science)
In mathematics and empirical science, quantification (or quantitation) is the act of counting and measuring that maps human sense observations and experiences into quantity, quantities. Quantification in this sense is fundamental to the scientific method. Natural science Some measure of the undisputed general importance of quantification in the natural sciences can be gleaned from the following comments: * "these are mere facts, but they are quantitative facts and the basis of science." * It seems to be held as universally true that "the foundation of quantification is measurement." * There is little doubt that "quantification provided a basis for the objectivity of science." * In ancient times, "musicians and artists ... rejected quantification, but merchants, by definition, quantified their affairs, in order to survive, made them visible on parchment and paper." * Any reasonable "comparison between Aristotle and Galileo shows clearly that there can be no unique lawfulness discov ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microscope
A microscope () is a laboratory equipment, laboratory instrument used to examine objects that are too small to be seen by the naked eye. Microscopy is the science of investigating small objects and structures using a microscope. Microscopic means being invisible to the eye unless aided by a microscope. There are many types of microscopes, and they may be grouped in different ways. One way is to describe the method an instrument uses to interact with a sample and produce images, either by sending a beam of light or electrons through a sample in its optical path, by detecting fluorescence, photon emissions from a sample, or by scanning across and a short distance from the surface of a sample using a probe. The most common microscope (and the first to be invented) is the optical microscope, which uses lenses to refract visible light that passed through a microtome, thinly sectioned sample to produce an observable image. Other major types of microscopes are the fluorescence micro ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cytometer
Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: * Cell counting * Cell sorting * Determining cell characteristics and function * Detecting microorganisms * Biomarker detection * Protein engineering detection * Diagnosis of health disorders such as blood cancers * Mea ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
German Language
German (, ) is a West Germanic language in the Indo-European language family, mainly spoken in Western Europe, Western and Central Europe. It is the majority and Official language, official (or co-official) language in Germany, Austria, Switzerland, and Liechtenstein. It is also an official language of Luxembourg, German-speaking Community of Belgium, Belgium and the Italian autonomous province of South Tyrol, as well as a recognized national language in Namibia. There are also notable German-speaking communities in other parts of Europe, including: Poland (Upper Silesia), the Czech Republic (North Bohemia), Denmark (South Jutland County, North Schleswig), Slovakia (Krahule), Germans of Romania, Romania, Hungary (Sopron), and France (European Collectivity of Alsace, Alsace). Overseas, sizeable communities of German-speakers are found in the Americas. German is one of the global language system, major languages of the world, with nearly 80 million native speakers and over 130 mi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Impedance Flow Cytometry
Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: * Cell counting * Cell sorting * Determining cell characteristics and function * Detecting microorganisms * Biomarker detection * Protein engineering detection * Diagnosis of health disorders such as blood cancers * Measu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
