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Cryoelectron Microscopy
Cryogenic electron microscopy (cryo-EM) is a transmission electron microscopy technique applied to samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of phases of ice#Crystal structure, vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution. This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy in the structural biology field. In 2017, the Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson (biologist), Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biom ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Titan Krios University Of Leeds
Titan most often refers to: * Titan (moon), the largest moon of Saturn * Titans, a race of deities in Greek mythology Titan or Titans may also refer to: Arts and entertainment Fictional entities Fictional locations * Titan in fiction, fictionalized depictions of the moon of Saturn * Titan (Marvel Comics location), a moon ** Titan (Marvel Cinematic Universe), its Marvel Cinematic Universe counterpart * Titan, a moon in the list of locations of the DC Universe * Titan, a '' Fighting Fantasy'' gamebooks world Fictional characters * Titan (Dark Horse Comics), a superhero * Titan (Imperial Guard), a Marvel Comics superhero * Titan (New Gods), from DC Comics' ''Darkseid's Elite'' * Titan, in the Infershia Pantheon * Titan, in '' Megamind'' * Titan, in '' Sym-Bionic Titan'' * King Titan, on ''Stingray'' (1964 TV series) Titan, Invincible (comics), an Image Comics character Fictional species and groups * Titan (''Dune'') * Titan (''Dungeons & Dragons'') * Titans (''Attack ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fahrenheit
The Fahrenheit scale () is a scale of temperature, temperature scale based on one proposed in 1724 by the German-Polish physicist Daniel Gabriel Fahrenheit (1686–1736). It uses the degree Fahrenheit (symbol: °F) as the unit. Several accounts of how he originally defined his scale exist, but the original paper suggests the lower defining point, 0 °F, was established as the freezing temperature of a solution of brine made from a mixture of water, ice, and ammonium chloride (a Salt (chemistry), salt). The other limit established was his best estimate of the average human body temperature, originally set at 90 °F, then 96 °F (about 2.6 °F less than the modern value due to a later redefinition of the scale). For much of the 20th century, the Fahrenheit scale was defined by two fixed points with a 180 °F separation: the temperature at which pure water freezes was defined as 32 °F and the boiling point of water was defined to be 212 °F, b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Semliki Forest Virus
Semliki Forest virus is an alphavirus found in central, eastern, and southern Africa. It was first isolated from mosquitoes in the Semliki Forest, Uganda by the Uganda Virus Research Institute in 1942 and described by Smithburn and Haddow. It is known to cause disease in animals and humans. The Semliki Forest virus is a positive-strand RNA virus with a genome of approximately 13,000 base pairs which encodes nine proteins. The 5’ two thirds of the genome encode four non-structural proteins concerned with RNA synthesis; the structural proteins are encoded in the 3’ one third. Of the structural proteins, the C proteins make up the icosahedral capsid, which is enveloped by a lipid bilayer derived from the host cell. The outermost surface of the virus is almost entirely covered by heterodimers of glycoproteins E1 and E2, arranged in interconnective trimers, which form an outer shell. Trimers are anchored in the membrane by an E2 cytoplasmic domain that associates with the nuc ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
T4 Bacteriophage
Escherichia virus T4 is a species of bacteriophages that infect ''Escherichia coli'' bacteria. It is a double-stranded DNA virus in the subfamily '' Tevenvirinae'' of the family '' Straboviridae''. T4 is capable of undergoing only a lytic life cycle and not the lysogenic life cycle. The species was formerly named T-even bacteriophage, a name which also encompasses, among other strains (or isolates), Enterobacteria phage T2, Enterobacteria phage T4 and Enterobacteria phage T6. Use in research Dating back to the 1940s and continuing today, T-even phages are considered the best studied model organisms. Model organisms are usually required to be simple with as few as five genes. Yet, T-even phages are in fact among the largest and highest complexity virus, in which these phage's genetic information is made up of around 300 genes. Coincident with their complexity, T-even viruses were found to have the unusual base hydroxymethylcytosine (HMC) in place of the nucleic acid base ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Adenoviridae
Adenoviruses (members of the family (biology), family ''Adenoviridae'') are medium-sized (90–100 nanometer, nm), nonenveloped (without an outer lipid bilayer) viruses with an icosahedral nucleocapsid containing a double-stranded DNA genome. Their name derives from their initial isolation from human adenoids in 1953. They have a broad range of vertebrate hosts; in humans, more than 50 distinct adenoviral serotypes have been found to cause a wide range of Adenovirus infection, illnesses, from mild respiratory infections in young children (the common cold) to life-threatening multi-organ disease in people with a Immunodeficiency, weakened immune system. Virology Classification This family contains the following genus, genera: * ''Aviadenovirus'' * ''Barthadenovirus'' * ''Ichtadenovirus'' * ''Mastadenovirus'' (including all human adenoviruses) * ''Siadenovirus'' * ''Testadenovirus'' Diversity In humans, currently there are 88 human adenoviruses (HAdVs) in seven species (H ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electron Diffraction
Electron diffraction is a generic term for phenomena associated with changes in the direction of electron beams due to elastic interactions with atoms. It occurs due to elastic scattering, when there is no change in the energy of the electrons. The negatively charged electrons are scattered due to Coulomb forces when they interact with both the positively charged atomic core and the negatively charged electrons around the atoms. The resulting map of the directions of the electrons far from the sample is called a diffraction pattern, see for instance Figure 1. Beyond patterns showing the directions of electrons, electron diffraction also plays a major role in the contrast of images in electron microscopes. This article provides an overview of electron diffraction and electron diffraction patterns, collective referred to by the generic name electron diffraction. This includes aspects of how in a general way electrons can act as waves, and diffract and interact with matter. It a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Amorphous Ice
Variations in pressure and temperature give rise to different phases of ice, which have varying properties and molecular geometries. Currently, twenty-one phases, including both crystalline and amorphous ices have been observed. In modern history, phases have been discovered through scientific research with various techniques including pressurization, force application, nucleation agents, and others. On Earth, most ice is found in the hexagonal Ice Ih phase. Less common phases may be found in the atmosphere and underground due to more extreme pressures and temperatures. Some phases are manufactured by humans for nano scale uses due to their properties. In space, amorphous ice is the most common form as confirmed by observation. Thus, it is theorized to be the most common phase in the universe. Various other phases could be found naturally in astronomical objects. Theory Most liquids under increased pressure freeze at ''higher'' temperatures because the pressure helps to hold ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cryogenics
In physics, cryogenics is the production and behaviour of materials at very low temperatures. The 13th International Institute of Refrigeration's (IIR) International Congress of Refrigeration (held in Washington, DC in 1971) endorsed a universal definition of "cryogenics" and "cryogenic" by accepting a threshold of to distinguish these terms from conventional refrigeration. This is a logical dividing line, since the normal boiling points of the so-called permanent gases (such as helium, hydrogen, neon, nitrogen, oxygen, and normal air) lie below 120 K, while the Freon refrigerants, hydrocarbons, and other common refrigerants have boiling points above 120 K. Discovery of superconducting materials with critical temperatures significantly above the boiling point of nitrogen has provided new interest in reliable, low-cost methods of producing high-temperature cryogenic refrigeration. The term "high temperature cryogenic" describes temperatures ranging from above the boili ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Vitrification
Vitrification (, via French ') is the full or partial transformation of a substance into a glass, that is to say, a non- crystalline or amorphous solid. Glasses differ from liquids structurally and glasses possess a higher degree of connectivity with the same Hausdorff dimensionality of bonds as crystals: dimH = 3. In the production of ceramics, vitrification is responsible for their impermeability to water. Vitrification is usually achieved by heating materials until they liquify, then cooling the liquid, often rapidly, so that it passes through the glass transition to form a glassy solid. Certain chemical reactions also result in glasses. In terms of chemistry, vitrification is characteristic for amorphous materials or disordered systems and occurs when bonding between elementary particles (atoms, molecules, forming blocks) becomes higher than a certain threshold value. Thermal fluctuations break the bonds; therefore, the lower the temperature, the higher the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
European Molecular Biology Laboratory
The European Molecular Biology Laboratory (EMBL) is an intergovernmental organization dedicated to molecular biology research and is supported by 29 member states, two prospect member states, and one associate member state. EMBL was created in 1974 and is funded by public research money from its member states. Research at EMBL is conducted by more than 110 independent research groups and service teams covering the spectrum of molecular biology. The Laboratory operates from six sites: the main laboratory in Heidelberg (Germany), and sites in Barcelona (Spain), Grenoble (France), Hamburg (Germany), Hinxton (the European Bioinformatics Institute (EBI), in England), and Rome (Italy). EMBL groups and laboratories perform basic research in molecular biology and molecular medicine as well as train scientists, students, and visitors. The organization aids in the development of services, new instruments and methods, and technology in its member states. Israel is the only full member stat ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Alasdair McDowall
Alasdair () is a Scottish Gaelic given name. The name is a Gaelic form of ''Alexander'' which has long been a popular name in Scotland. The personal name ''Alasdair'' is often Anglicised as '' Alistair'', '' Alastair'', and ''Alaster''.''A Dictionary of First Names'', Oxford University Press, People named Alasdair * Alexander of Argyll (died 1310), Scottish magnate *Alasdair Allan (born 1971), Scottish politician * Alasdair Beckett-King (born 1984), English stand-up comedian * Alasdair Boyle (born 1945), Scottish rugby union player * Alasdair Breckenridge (1937–2019), Scottish pharmacologist * Alasdair Caimbeul (born 1941), Scottish playwright, short story writer, and novelist * Alasdair Clayre (1935–1984), British author and broadcaster * Alasdair Cochrane (born 1978), British political theorist and ethicist * Alasdair Dickinson (born 1983), Scottish rugby union coach * Alasdair Drysdale (born 1950), Scottish-American geographer and professor * Alasdair Duncan (born 1982), Aus ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Valine
Valine (symbol Val or V) is an α-amino acid that is used in the biosynthesis of proteins. It contains an α- amino group (which is in the protonated −NH3+ form under biological conditions), an α- carboxylic acid group (which is in the deprotonated −COO− form under biological conditions), and a side chain isopropyl group, making it a non-polar aliphatic amino acid. Valine is essential in humans, meaning the body cannot synthesize it; it must be obtained from dietary sources which are foods that contain proteins, such as meats, dairy products, soy products, beans and legumes. It is encoded by all codons starting with GU (GUU, GUC, GUA, and GUG). History and etymology Valine was first isolated from casein in 1901 by Hermann Emil Fischer. The name valine comes from its structural similarity to valeric acid, which in turn is named after the plant valerian due to the presence of the acid in the roots of the plant. Nomenclature According to IUPAC, carbon atoms forming v ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
