Bacillus Thuringiensis Israelensis
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Bacillus Thuringiensis Israelensis
''Bacillus thuringiensis'' serotype ''israelensis'' (Bti) is a group of bacteria used as biological control agents for larvae stages of certain dipterans. Bti produces toxins which are effective in killing various species of mosquitoes, fungus gnats, and blackflies, while having almost no effect on other organisms. The major advantage of '' B. thuringiensis'' products is that they are thought to affect few non-target species. However, even though Bti may have minimal direct effects on non-target organisms, it may potentially be associated with knock-on effects on food webs and other ecosystem properties, including biodiversity and ecosystem functioning. Bti strains possess the pBtoxis plasmid which encodes numerous Cry (a δ-endotoxin) and Cyt toxins, including Cry4, Cry10, Cry11, Cyt1, and Cyt2. The crystal aggregation which these toxins form contains at least four major toxic components, but the extent to which each Cry and Cyt protein is represented is not known and li ...
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Henry De Barjac
Henry may refer to: People *Henry (given name) *Henry (surname) * Henry Lau, Canadian singer and musician who performs under the mononym Henry Royalty * Portuguese royalty ** King-Cardinal Henry, King of Portugal ** Henry, Count of Portugal, Henry of Burgundy, Count of Portugal (father of Portugal's first king) ** Prince Henry the Navigator, Infante of Portugal ** Infante Henrique, Duke of Coimbra (born 1949), the sixth in line to Portuguese throne * King of Germany **Henry the Fowler (876–936), first king of Germany * King of Scots (in name, at least) ** Henry Stuart, Lord Darnley (1545/6–1567), consort of Mary, queen of Scots ** Henry Benedict Stuart, the 'Cardinal Duke of York', brother of Bonnie Prince Charlie, who was hailed by Jacobites as Henry IX * Four kings of Castile: **Henry I of Castile **Henry II of Castile **Henry III of Castile **Henry IV of Castile * Five kings of France, spelt ''Henri'' in Modern French since the Renaissance to italianize the name and to ...
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Plasmid
A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that benefit the survival of the organism and confer selective advantage such as antibiotic resistance. While chromosomes are large and contain all the essential genetic information for living under normal conditions, plasmids are usually very small and contain only additional genes that may be useful in certain situations or conditions. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms. In the laboratory, plasmids may be introduced into a cell via transformation. Synthetic plasmids are available for procurement over the int ...
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Malaria
Malaria is a mosquito-borne infectious disease that affects humans and other animals. Malaria causes symptoms that typically include fever, tiredness, vomiting, and headaches. In severe cases, it can cause jaundice, seizures, coma, or death. Symptoms usually begin ten to fifteen days after being bitten by an infected mosquito. If not properly treated, people may have recurrences of the disease months later. In those who have recently survived an infection, reinfection usually causes milder symptoms. This partial resistance disappears over months to years if the person has no continuing exposure to malaria. Malaria is caused by single-celled microorganisms of the ''Plasmodium'' group. It is spread exclusively through bites of infected ''Anopheles'' mosquitoes. The mosquito bite introduces the parasites from the mosquito's saliva into a person's blood. The parasites travel to the liver where they mature and reproduce. Five species of ''Plasmodium'' can infect and be spread by h ...
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Cell Membrane
The cell membrane (also known as the plasma membrane (PM) or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates and protects the interior of all cells from the outside environment (the extracellular space). The cell membrane consists of a lipid bilayer, made up of two layers of phospholipids with cholesterols (a lipid component) interspersed between them, maintaining appropriate membrane fluidity at various temperatures. The membrane also contains membrane proteins, including integral proteins that span the membrane and serve as membrane transporters, and peripheral proteins that loosely attach to the outer (peripheral) side of the cell membrane, acting as enzymes to facilitate interaction with the cell's environment. Glycolipids embedded in the outer lipid layer serve a similar purpose. The cell membrane controls the movement of substances in and out of cells and organelles, being selectively permeable to io ...
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Epithelium
Epithelium or epithelial tissue is one of the four basic types of animal tissue, along with connective tissue, muscle tissue and nervous tissue. It is a thin, continuous, protective layer of compactly packed cells with a little intercellular matrix. Epithelial tissues line the outer surfaces of organs and blood vessels throughout the body, as well as the inner surfaces of cavities in many internal organs. An example is the epidermis, the outermost layer of the skin. There are three principal shapes of epithelial cell: squamous (scaly), columnar, and cuboidal. These can be arranged in a singular layer of cells as simple epithelium, either squamous, columnar, or cuboidal, or in layers of two or more cells deep as stratified (layered), or ''compound'', either squamous, columnar or cuboidal. In some tissues, a layer of columnar cells may appear to be stratified due to the placement of the nuclei. This sort of tissue is called pseudostratified. All glands are made up of epit ...
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Lysis
Lysis ( ) is the breaking down of the membrane of a cell, often by viral, enzymic, or osmotic (that is, "lytic" ) mechanisms that compromise its integrity. A fluid containing the contents of lysed cells is called a ''lysate''. In molecular biology, biochemistry, and cell biology laboratories, cell cultures may be subjected to lysis in the process of purifying their components, as in protein purification, DNA extraction, RNA extraction, or in purifying organelles. Many species of bacteria are subject to lysis by the enzyme lysozyme, found in animal saliva, egg white, and other secretions. Phage lytic enzymes (lysins) produced during bacteriophage infection are responsible for the ability of these viruses to lyse bacterial cells. Penicillin and related β-lactam antibiotics cause the death of bacteria through enzyme-mediated lysis that occurs after the drug causes the bacterium to form a defective cell wall. If the cell wall is completely lost and the penicillin was ...
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Pore-forming Toxins
Pore-forming proteins (PFTs, also known as pore-forming toxins) are usually produced by bacteria, and include a number of protein exotoxins but may also be produced by other organisms such as apple snails that produce perivitellin-2 or earthworms, who produce lysenin. They are frequently cytotoxic (i.e., they kill cells), as they create unregulated pores in the membrane of targeted cells. Types PFTs can be divided into two categories, depending on the alpha-helical or beta-barrel architecture of their transmembrane channel that can consist either of * Alpha-pore-forming toxins ** e.g., Haemolysin E family, actinoporins, Corynebacterial porin B, Cytolysin A of ''E. coli''. * Beta-barrel pore-forming toxins ** e.g. α-hemolysin (Fig 1), PVL – Panton-Valentine leukocidin, various insecticidal toxins. Other categories: * Large beta-barrel pore-forming toxins ** MACPF and Cholesterol-dependent cytolysins (CDCs), gasdermin * Binary toxins ** e.g., Anthrax toxin, Pleuroto ...
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δ-endotoxin
Delta endotoxins (δ-endotoxins) are pore-forming toxins produced by '' Bacillus thuringiensis'' species of bacteria. They are useful for their insecticidal action and are the primary toxin produced by Bt maize/corn. During spore formation the bacteria produce crystals of such proteins (hence the name Cry toxins) that are also known as parasporal bodies, next to the endospores; as a result some members are known as a parasporin. The Cyt (cytolytic) toxin group is a group of delta-endotoxins different from the Cry group. Mechanism of action When an insect ingests these proteins, they are activated by proteolytic cleavage. The N-terminus is cleaved in all of the proteins and a C-terminal extension is cleaved in some members. Once activated, the endotoxin binds to the gut epithelium and causes cell lysis by the formation of cation-selective channels, which leads to death. For many years there was no clarity as to the relationship between aminopeptidase N and Bt toxins. Al ...
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Food Web
A food web is the natural interconnection of food chains and a graphical representation of what-eats-what in an ecological community. Another name for food web is consumer-resource system. Ecologists can broadly lump all life forms into one of two categories called trophic levels: 1) the autotrophs, and 2) the heterotrophs. To maintain their bodies, grow, develop, and to reproduce, autotrophs produce organic matter from inorganic substances, including both minerals and gases such as carbon dioxide. These chemical reactions require energy, which mainly comes from the Sun and largely by photosynthesis, although a very small amount comes from bioelectrogenesis in wetlands, and mineral electron donors in hydrothermal vents and hot springs. These trophic levels are not binary, but form a gradient that includes complete autotrophs, which obtain their sole source of carbon from the atmosphere, mixotrophs (such as carnivorous plants), which are autotrophic organisms that partial ...
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Black Fly
A black fly or blackfly (sometimes called a buffalo gnat, turkey gnat, or white socks) is any member of the family Simuliidae of the Culicomorpha infraorder. It is related to the Ceratopogonidae, Chironomidae, and Thaumaleidae. Over 2,200 species of black flies have been formally named, of which 15 are extinct. They are divided into two subfamilies: Parasimuliinae contains only one genus and four species; Simuliinae contains all the rest. Over 1,800 of the species belong to the genus '' Simulium''. Most black flies gain nourishment by feeding on the blood of mammals, including humans, although the males feed mainly on nectar. They are usually small, black or gray, with short legs, and antennae. They are a common nuisance for humans, and many U.S. states have programs to suppress the black fly population. They spread several diseases, including river blindness in Africa (''Simulium damnosum'' and ''S. neavei'') and the Americas (''S. callidum'' and ''S. metallicum'' in Cent ...
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Fungus Gnat
Fungus gnats are small, dark, short-lived gnats, of the families Sciaridae, Diadocidiidae, Ditomyiidae, Keroplatidae, Bolitophilidae, and Mycetophilidae (order Diptera); they comprise six of the seven families placed in the superfamily Sciaroidea. Description The larvae of most species feed on fungi growing on soil, helping in the decomposition of organic matter. However some species are predatory, including those in the genus '' Arachnocampa'' of family Keroplatidae – the "glowworms" of Australia and New Zealand. The adults are long, and are occasionally pollinators of plants and carriers of mushroom spores. They also may carry diseases such as pythium (which causes " damping-off" to kill seedlings) on their feet. Most fungus gnats are weak fliers, and can often be seen walking rapidly over plants and soil, rather than flying. However when airborne, the gnats may be quite annoying to humans by flying into their faces, eyes, and noses, both indoors and outdoors. T ...
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