|
Biacore
Biacore was a life science products company based in Sweden. In June 2006 Biacore was sold for $390 million and became a product brand under GE Healthcare life Sciences, which became Cytiva in April 2020. Biacore products measure biomolecular interactions, including protein-protein interactions, small molecule/fragment-protein interactions, etc. Its technology is often used to measure not only binding affinities, but kinetic rate constants and thermodynamics as well. The technology is based on surface plasmon resonance (SPR), an optical phenomenon that enables detection of unlabeled interactants in real time. The SPR-based biosensors can be used in determination of active concentration as well as characterization of molecular interactions in terms of both affinity and chemical kinetics. History Biacore was founded in 1984 under the name of Pharmacia Biosensor AB, by researchers from Pharmacia, Linköping Institute of Technology and the Swedish National Defence Research Institu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Surface Plasmon Resonance
Surface plasmon resonance (SPR) is a phenomenon that occurs where electrons in a thin metal sheet become excited by light that is directed to the sheet with a particular angle of incidence (optics), angle of incidence, and then travel parallel to the sheet. Assuming a constant light source wavelength and that the metal sheet is thin, the angle of incidence that triggers SPR is related to the refractive index of the material and even a small change in the refractive index will cause SPR to not be observed. This makes SPR a possible technique for detecting particular substances (analytes) and SPR biosensors have been developed to detect various important biomarkers. Explanation The surface plasmon polariton is a non-radiative Surface wave#Electromagnetic waves, electromagnetic surface wave that propagates in a direction parallel to the negative permittivity/dielectric material interface. Since the wave is on the boundary of the conductor and the external medium (air, water or vacuu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methods To Investigate Protein–protein Interactions
There are many methods to investigate protein–protein interactions which are the physical contacts of high specificity established between two or more protein molecules involving electrostatic forces and hydrophobic effects. Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. A high sensitivity means that many of the interactions that occur are detected by the screen. A high specificity indicates that most of the interactions detected by the screen are occurring in reality. Biochemical methods Co-immunoprecipitation is considered to be the gold standard assay for protein–protein interactions, especially when it is performed with endogenous (not overexpressed and not tagged) proteins. The protein of interest is isolated with a specific antibody. Interaction partners which stick to this protein are subsequently identified by Western blotting. Interactions detected by this approach are considered ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Uppsala
Uppsala ( ; ; archaically spelled ''Upsala'') is the capital of Uppsala County and the List of urban areas in Sweden by population, fourth-largest city in Sweden, after Stockholm, Gothenburg, and Malmö. It had 177,074 inhabitants in 2019. Located north of the capital Stockholm, it is also the seat of Uppsala Municipality. Since 1164, Uppsala has been the ecclesiology, ecclesiastical centre of Sweden, being the seat of the Archbishop of Uppsala, Archbishop of the Church of Sweden. Uppsala is home to Scandinavia's largest cathedral – Uppsala Cathedral, which was the frequent site of the coronation of the Swedish monarch until the late 19th century. Uppsala Castle, built by King Gustav I of Sweden, Gustav Vasa, served as one of the royal residences of the Swedish monarchs, and was expanded several times over its history, making Uppsala the secondary capital of Sweden during its Swedish Empire, greatest extent. Today, it serves as the residence of the Governor of Uppsala County ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sweden
Sweden, formally the Kingdom of Sweden, is a Nordic countries, Nordic country located on the Scandinavian Peninsula in Northern Europe. It borders Norway to the west and north, and Finland to the east. At , Sweden is the largest Nordic country by both area and population, and is the List of European countries by area, fifth-largest country in Europe. Its capital and largest city is Stockholm. Sweden has a population of 10.6 million, and a low population density of ; 88% of Swedes reside in urban areas. They are mostly in the central and southern half of the country. Sweden's urban areas together cover 1.5% of its land area. Sweden has a diverse Climate of Sweden, climate owing to the length of the country, which ranges from 55th parallel north, 55°N to 69th parallel north, 69°N. Sweden has been inhabited since Prehistoric Sweden, prehistoric times around 12,000 BC. The inhabitants emerged as the Geats () and Swedes (tribe), Swedes (), who formed part of the sea-faring peopl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Life Science
Life, also known as biota, refers to matter that has biological processes, such as signaling and self-sustaining processes. It is defined descriptively by the capacity for homeostasis, organisation, metabolism, growth, adaptation, response to stimuli, and reproduction. All life over time eventually reaches a state of death, and none is immortal. Many philosophical definitions of living systems have been proposed, such as self-organizing systems. Viruses in particular make definition difficult as they replicate only in host cells. Life exists all over the Earth in air, water, and soil, with many ecosystems forming the biosphere. Some of these are harsh environments occupied only by extremophiles. Life has been studied since ancient times, with theories such as Empedocles's materialism asserting that it was composed of four eternal elements, and Aristotle's hylomorphism asserting that living things have souls and embody both form and matter. Life originated at least ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Affinity (pharmacology)
In biochemistry and pharmacology, a ligand is a substance that forms a complex with a biomolecule to serve a biological purpose. The etymology stems from Latin ''ligare'', which means 'to bind'. In protein-ligand binding, the ligand is usually a molecule which produces a signal by binding to a site on a target protein. The binding typically results in a change of conformational isomerism (conformation) of the target protein. In DNA-ligand binding studies, the ligand can be a small molecule, ion, or protein which binds to the DNA double helix. The relationship between ligand and binding partner is a function of charge, hydrophobicity, and molecular structure. Binding occurs by intermolecular forces, such as ionic bonds, hydrogen bonds and Van der Waals forces. The association or docking is actually reversible through dissociation. Measurably irreversible covalent bonding between a ligand and target molecule is atypical in biological systems. In contrast to the definition o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chemical Kinetics
Chemical kinetics, also known as reaction kinetics, is the branch of physical chemistry that is concerned with understanding the rates of chemical reactions. It is different from chemical thermodynamics, which deals with the direction in which a reaction occurs but in itself tells nothing about its rate. Chemical kinetics includes investigations of how experimental conditions influence the speed of a chemical reaction and yield information about the reaction's mechanism and transition states, as well as the construction of mathematical models that also can describe the characteristics of a chemical reaction. History The pioneering work of chemical kinetics was done by German chemist Ludwig Wilhelmy in 1850. He experimentally studied the rate of inversion of sucrose and he used integrated rate law for the determination of the reaction kinetics of this reaction. His work was noticed 34 years later by Wilhelm Ostwald. In 1864, Peter Waage and Cato Guldberg published the law ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pharmacia
Pharmacia was a pharmaceutical and biotechnological company in Sweden that merged with the American pharmaceutical company Upjohn in 1995. History Pharmacia company was founded in 1911 in Stockholm, Sweden by pharmacist Gustav Felix Grönfeldt at the ''Elgen'' Pharmacy. The company was named after the Greek word φαρμακεία, transliterated ''pharmakeia'', which means 'sorcery'. In the company's early days, much of its profits were derived from the "miracle medicine" Phospho-Energon. During World War II, Swedish chemist Björn Ingelman (who worked for Arne Tiselius at Uppsala university) researched various uses for the polysaccharide dextran. Together with the medical researcher Anders Grönwall, he discovered that dextran could be used as a replacement for blood plasma in blood transfusions, for which there could be a large need in wartime. Pharmacia, which then was still a small company, was contacted in 1943 and its CEO Elis Göth was very interested. The product M ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Linköping Institute Of Technology
Linköping ( , ) is a city in southern Sweden, with around 167,000 inhabitants as of 2024. It is the seat of Linköping Municipality and the capital of Östergötland County. Linköping is also the episcopal see of the Diocese of Linköping (Church of Sweden) and is well known for its cathedral. Linköping is the center of an old cultural region and celebrated its 700th anniversary in 1987. Dominating the city's skyline from afar is the steeple of Linköping Cathedral, the cathedral (). Nowadays, Linköping is known for its Linköping University, university and its High tech, high-technology industry. Linköping wants to create a sustainable development of the city and therefore plans to become a Carbon neutrality, carbon-neutral community by 2025. Located on the Östergötland Plain, Linköping is closely linked to Norrköping, roughly to the east, near the sea. History The city is possibly named after the ''Lionga thing, Lionga ting'' assembly which according to Medieval ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Swedish National Defence Research Institute
Swedish National Defence Research Institute (, FOA) was a Swedish government agency in defense research existing from 1945 to 31 December 2000. It was amalgamated with the National Aeronautical Research Institute (FFA) into the Swedish Defence Research Agency (FOI) which was established on 1 January 2001. History The National Defence Research Institute (FOA) was established in 1945 (Instruction TS C:II No. 24), and took over operations at the Swedish Armed Forces Chemical Institute (''Försvarsväsendets kemiska anstalt'', FKA), the Military Physics Institute and the Telecommunications Technical Working Group of the Swedish Board of Inventions (''Statens uppfinnarnämnds teletekniska arbetsgrupp'', SUN), that is, essentially all technical and scientific research outside the industry. FOA was organized in three research departments, FOA 1 chemistry/medicine, FOA 2 general physics and FOA 3 telecommunications and an office. In 1959 FOA 4 (nuclear physics and nuclear chemistry) ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Swedish Defence Research Agency
The Swedish Defence Research Agency (, FOI; literal translation: ''Total Defence Research Institute'') is a government agency in Sweden for total defence research and development. FOI has its headquarters in Kista (Stockholm). Other FOI offices or research centres are located in Grindsjön, Linköping, and Umeå. History FOI was created in 2001 by combining the Swedish National Defence Research Institute (FOA) with the National Aeronautical Research Institute (FFA). The first of these agencies, FFA, had been created in 1940 in Bromma, Stockholm as a governmental research institute for the Swedish aviation industry, large parts of which were devoted to military aircraft. The second, FOA, had been created in 1945 from three existing organisations: * The Swedish Armed Forces Chemical Institute (''Försvarsväsendets kemiska anstalt'', FKA), a government agency created in 1937 and located in Ursvik, Sundbyberg Municipality. FKA had predecessors in chemical warfare and chemical ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
