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4Pi STED Microscopy
The 4Pi-STED-microscope is the result of combining the two unrelated concepts of Stimulated emission depletion (STED) microscopy and 4Pi-microscopy. Here, a fluorescent sample is placed in the common focus of two opposing lenses, but excitation and detection are performed through a single lens (4Pi mode A). The green excitation pulse is immediately followed by a red STED-pulse, which enters the focal region through both lenses inducing stimulated emission of the excited fluorescent molecules to the ground state. To permit fluorescence emission from the center but suppress it from neighbouring regions it is useful to phase shift In physics and mathematics, the phase of a periodic function F of some real variable t (such as time) is an angle-like quantity representing the fraction of the cycle covered up to t. It is denoted \phi(t) and expressed in such a scale that it ... the STED beam to have a minimum at the center. External links Technical details Microscopy {{ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
STED Microscopy
Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy. It creates super-resolution images by the selective deactivation of fluorophores, minimizing the area of illumination at the focal point, and thus enhancing the achievable resolution for a given system. It was developed by Stefan W. Hell and Jan Wichmann in 1994, and was first experimentally demonstrated by Hell and Thomas Klar in 1999. Hell was awarded the Nobel Prize in Chemistry in 2014 for its development. In 1986, V.A. Okhonin (Institute of Biophysics, USSR Academy of Sciences, Siberian Branch, Krasnoyarsk) had patented the STED idea. This patent was unknown to Hell and Wichmann in 1994. STED microscopy is one of several types of super resolution microscopy techniques that have recently been developed to bypass the diffraction limit of light microscopy to increase resolution. STED is a deterministic functional technique that exploits the non-linear response o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
4Pi Microscope
A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution. With it the typical range of the axial resolution of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy. Working principle The improvement in resolution is achieved by using two opposing objective lenses, which both are focused to the same geometrical location. Also the difference in optical path length through each of the two objective lenses is carefully aligned to be minimal. By this method, molecules residing in the common focal area of both objectives can be illuminated coherently from both sides and the reflected or emitted light can also be collected coherently, i.e. coherent superposition of emitted light on the detector is possible. The solid angle \Omega that is used for illumination and detection is increased and approaches its maximum. In this case ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescent
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescent materials, which continue to emit light for some time after. Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Focus (optics)
In geometrical optics, a focus, also called an image point, is a point where light rays originating from a point on the object converge. Although the focus is conceptually a point, physically the focus has a spatial extent, called the blur circle. This non-ideal focusing may be caused by aberrations of the imaging optics. In the absence of significant aberrations, the smallest possible blur circle is the Airy disc, which is caused by diffraction from the optical system's aperture. Aberrations tend to worsen as the aperture diameter increases, while the Airy circle is smallest for large apertures. An image, or image point or region, is in focus if light from object points is converged almost as much as possible in the image, and out of focus if light is not well converged. The border between these is sometimes defined using a " circle of confusion" criterion. A principal focus or focal point is a special focus: * For a lens, or a spherical or parabolic mirror, it is a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Lens (optics)
A lens is a transmissive optical device which focuses or disperses a light beam by means of refraction. A simple lens consists of a single piece of transparent material, while a compound lens consists of several simple lenses (''elements''), usually arranged along a common axis. Lenses are made from materials such as glass or plastic, and are ground and polished or molded to a desired shape. A lens can focus light to form an image, unlike a prism, which refracts light without focusing. Devices that similarly focus or disperse waves and radiation other than visible light are also called lenses, such as microwave lenses, electron lenses, acoustic lenses, or explosive lenses. Lenses are used in various imaging devices like telescopes, binoculars and cameras. They are also used as visual aids in glasses to correct defects of vision such as myopia and hypermetropia. History The word ''lens'' comes from '' lēns'', the Latin name of the lentil (a seed of a lent ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Excited State
In quantum mechanics, an excited state of a system (such as an atom, molecule or nucleus) is any quantum state of the system that has a higher energy than the ground state (that is, more energy than the absolute minimum). Excitation refers to an increase in energy level above a chosen starting point, usually the ground state, but sometimes an already excited state. The temperature of a group of particles is indicative of the level of excitation (with the notable exception of systems that exhibit negative temperature). The lifetime of a system in an excited state is usually short: spontaneous or induced emission of a quantum of energy (such as a photon or a phonon) usually occurs shortly after the system is promoted to the excited state, returning the system to a state with lower energy (a less excited state or the ground state). This return to a lower energy level is often loosely described as decay and is the inverse of excitation. Long-lived excited states are often calle ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Detection
{{Unreferenced, date=March 2018 In general, detection is the action of accessing information without specific cooperation from with the sender. In the history of radio communications, the term " detector" was first used for a device that detected the simple presence or absence of a radio signal, since all communications were in Morse code. The term is still in use today to describe a component that extracts a particular signal from all of the electromagnetic waves present. Detection is usually based on the frequency of the carrier signal, as in the familiar frequencies of radio broadcasting, but it may also involve filtering a faint signal from noise, as in radio astronomy, or reconstructing a hidden signal, as in steganography. In optoelectronics, "detection" means converting a received optical input to an electrical output. For example, the light signal received through an optical fiber is converted to an electrical signal in a detector such as a photodiode. In steganography, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pulse (physics)
In physics, a pulse is a generic term describing a single disturbance that moves through a transmission medium. This medium may be vacuum (in the case of electromagnetic radiation) or matter, and may be indefinitely large or finite. Pulse reflection Consider a pulse moving through a medium - perhaps through a rope or a slinky. When the pulse reaches the end of that medium, what happens to it depends on whether the medium is fixed in space or free to move at its end. For example, if the pulse is moving through a rope and the end of the rope is held firmly by a person, then it is said that the pulse is approaching a fixed end. On the other hand, if the end of the rope is fixed to a stick such that it is free to move up or down along the stick when the pulse reaches its end, then it is said that the pulse is approaching a free end. Free end A pulse will reflect off a free end and return with the same direction of displacement that it had before reflection. That is, a pulse with ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Emission Spectrum
The emission spectrum of a chemical element or chemical compound is the spectrum of frequencies of electromagnetic radiation emitted due to an electron making a transition from a high energy state to a lower energy state. The photon energy of the emitted photon is equal to the energy difference between the two states. There are many possible electron transitions for each atom, and each transition has a specific energy difference. This collection of different transitions, leading to different radiated wavelengths, make up an emission spectrum. Each element's emission spectrum is unique. Therefore, spectroscopy can be used to identify elements in matter of unknown composition. Similarly, the emission spectra of molecules can be used in chemical analysis of substances. Emission In physics, emission is the process by which a higher energy quantum mechanical state of a particle becomes converted to a lower one through the emission of a photon, resulting in the production of light. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Molecule
A molecule is a group of two or more atoms held together by attractive forces known as chemical bonds; depending on context, the term may or may not include ions which satisfy this criterion. In quantum physics, organic chemistry, and biochemistry, the distinction from ions is dropped and ''molecule'' is often used when referring to polyatomic ions. A molecule may be homonuclear, that is, it consists of atoms of one chemical element, e.g. two atoms in the oxygen molecule (O2); or it may be heteronuclear, a chemical compound composed of more than one element, e.g. water (molecule), water (two hydrogen atoms and one oxygen atom; H2O). In the kinetic theory of gases, the term ''molecule'' is often used for any gaseous particle regardless of its composition. This relaxes the requirement that a molecule contains two or more atoms, since the noble gases are individual atoms. Atoms and complexes connected by non-covalent interactions, such as hydrogen bonds or ionic bonds, are typic ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ground State
The ground state of a quantum-mechanical system is its stationary state of lowest energy; the energy of the ground state is known as the zero-point energy of the system. An excited state is any state with energy greater than the ground state. In quantum field theory, the ground state is usually called the vacuum state or the vacuum. If more than one ground state exists, they are said to be degenerate. Many systems have degenerate ground states. Degeneracy occurs whenever there exists a unitary operator that acts non-trivially on a ground state and commutes with the Hamiltonian of the system. According to the third law of thermodynamics, a system at absolute zero temperature exists in its ground state; thus, its entropy is determined by the degeneracy of the ground state. Many systems, such as a perfect crystal lattice, have a unique ground state and therefore have zero entropy at absolute zero. It is also possible for the highest excited state to have absolute zero te ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phase Shift
In physics and mathematics, the phase of a periodic function F of some real variable t (such as time) is an angle-like quantity representing the fraction of the cycle covered up to t. It is denoted \phi(t) and expressed in such a scale that it varies by one full turn as the variable t goes through each period (and F(t) goes through each complete cycle). It may be measured in any angular unit such as degrees or radians, thus increasing by 360° or 2\pi as the variable t completes a full period. This convention is especially appropriate for a sinusoidal function, since its value at any argument t then can be expressed as \phi(t), the sine of the phase, multiplied by some factor (the amplitude of the sinusoid). (The cosine may be used instead of sine, depending on where one considers each period to start.) Usually, whole turns are ignored when expressing the phase; so that \phi(t) is also a periodic function, with the same period as F, that repeatedly scans the same range o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
